Recombinant Human E-Selectin/CD62E Protein, CF Summary
Optimal dilutions should be determined by each laboratory for each application.
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS containing Calcium and Magnesium with Sorbitol.|
|Reconstitution||Reconstitute at 1 mg/mL in sterile, deionized water.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107-115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha, and demonstrates peak expression at 4 hours, and decay at 24 hours, in response to activation.
E‑Selectin ligands, expressed on neutrophils, monocytes, and a subset of memory T-cells, are sialylated, fucosylated molecules which bind to the lectin domain of E-Selectin. Immunocytochemical techniques have demonstrated the expression of E-Selectin on healthy and diseased tissue.
E-Selectin mediates the attachment of flowing leukocytes to the blood vessel wall during inflammation by binding to E‑Selectin ligands on leukocytes. These interactions are labile, and permit leukocytes to roll along the vascular endothelium in the direction of blood flow. This initial interaction is followed by a stronger interaction involving ICAM-1 and VCAM-1 that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue.
ELISA techniques have shown that detectable levels of soluble E-Selectin are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of E-Selectin may be elevated in subjects with a variety of pathological conditions.
Citations for Recombinant Human E-Selectin/CD62E Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 9
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TRAIL-coated leukocytes to kill circulating tumor cells in the flowing blood from prostate cancer patients
Authors: N Ortiz-Oter, JR Marshall, A Glenn, J Matloubieh, J Joseph, DM Sahasrabud, EM Messing, MR King
BMC Cancer, 2021;21(1):898.
Sample Types: Whole Cells
Lectin nanoparticle assays for detecting breast cancer-associated glycovariants of cancer antigen 15-3 (CA15-3) in human plasma
Authors: J Terävä, L Tiainen, U Lamminmäki, PL Kellokumpu, K Pettersson, K Gidwani
PLoS ONE, 2019;14(7):e0219480.
Sample Types: Natural Protein
Multivalent binding and biomimetic cell rolling improves the sensitivity and specificity of circulating tumor cellï¿½capture
Authors: JH Myung, MJ Eblan, JM Caster, S Park, MJ Poellmann, K Wang, JE Tepper, KA Tam, SM Miller, C Shen, RC Chen, T Zhang, B Chera, AZ Wang, S Hong
Clin. Cancer Res., 2018;0(0):.
Sample Types: Whole Cells
Cholesterol Regulates Monocyte Rolling through CD44 Distribution
Authors: AK Saha, P Osmulski, SF Dallo, M Gaczynska, TH Huang, AK Ramasubram
Biophys. J., 2017;112(7):1481-1488.
Sample Types: Complex Sample Type
Blocking the adhesion cascade at the premetastatic niche for prevention of breast cancer metastasis.
Authors: Kang S, Hasan N, Mann A, Zheng W, Zhao L, Morris L, Zhu W, Zhao Y, Suh K, Dooley W, Volk D, Gorenstein D, Cristofanilli M, Rui H, Tanaka T
Mol Ther, 2015;23(6):1044-54.
Sample Types: Cell Lysates
c-FOS suppresses ovarian cancer progression by changing adhesion.
Authors: Oliveira-Ferrer L, Rossler K, Haustein V, Schroder C, Wicklein D, Maltseva D, Khaustova N, Samatov T, Tonevitsky A, Mahner S, Janicke F, Schumacher U, Milde-Langosch K
Br J Cancer, 2014;110(3):753-63.
Regulation of leukocyte recruitment by the long pentraxin PTX3.
Authors: Deban L, Russo RC, Sironi M, Moalli F, Scanziani M, Zambelli V, Cuccovillo I, Bastone A, Gobbi M, Valentino S, Doni A, Garlanda C, Danese S, Salvatori G, Sassano M, Evangelista V, Rossi B, Zenaro E, Constantin G, Laudanna C, Bottazzi B, Mantovani A
Nat. Immunol., 2010;11(4):328-34.
Sample Types: Recombinant Protein
Applications: Binding Assay
Development and validation of sandwich ELISA microarrays with minimal assay interference.
Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
J. Proteome Res., 2008;7(6):2406-14.
Sample Types: N/A
Applications: ELISA (Standard)
E-selectin regulates gene expression in metastatic colorectal carcinoma cells and enhances HMGB1 release.
Authors: Aychek T, Miller K, Sagi-Assif O, Levy-Nissenbaum O, Israeli-Amit M, Pasmanik-Chor M, Jacob-Hirsch J, Amariglio N, Rechavi G, Witz IP
Int. J. Cancer, 2008;123(8):1741-50.
Sample Types: Whole Cells
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