Recombinant Human ECM1 Protein, CF

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R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human ECM1 Protein, CF Summary

Product Specifications

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Measured by the ability of the immobilized protein to support the adhesion of B16‑F1 mouse melanoma cells. When 5 x 104 cells/well are added to Recombinant Human ECM-1 coated plates (10 µg/mL with 100 µL/well), >30% will adhere after 30 minutes at 37 °C.
Optimal concentration depends on cell type as well as the application or research objective.
Mouse myeloma cell line, NS0-derived human ECM1 protein
Ala20-Glu540, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Predicted Molecular Mass
59.7 kDa
77-87 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: ECM1

Extracellular matrix protein-1 (ECM-1) is an 85 kDa, secreted glycoprotein important in connective tissue organization (1-3). Of three identified splice variants the 540 amino acid (aa) form, ECM-1a, is the most widely expressed, with the highest expression in the placenta and heart (2). ECM-1b (415 aa) is found only in tonsil and associated with suprabasal keratinocytes (2, 4). Since ECM-1b expression is differentiation-dependent, a role in terminal keratinocyte differentiation has been suggested (4). ECM-1c (559 aa) accounts for approximately 15% of skin ECM-1 (5). Human ECM-1a contains a 19 aa signal peptide and a 521 aa secreted portion that includes an N-terminal proline-rich, cysteine-free region, two tandem repeat domains, and a C-terminal domain. There are six repeats of a CC(X7-10)C motif
(x = any aa) within the tandem repeat and C-terminal domains. These motifs are involved in ligand binding to members of the albumin family, and are expected to form two (in ECM-1b) or three (in ECM-1a) “double loop” structures (2). Mature human ECM-1a shows 69%, 71%, 72% and 76% aa identity with corresponding isoforms of mouse, rat, canine, and bovine ECM-1, respectively. ECM-1 is over-expressed in many malignant epithelial tumors and has demonstrated angiogenic activity (6, 7). A variety of ECM-1 mutations, mainly within the first tandem repeat, are considered causative of lipoid proteinosis, a condition showing thickened and irregular extracellular matrix within connective tissue (8). In the autoimmune condition lichen sclerosis, auto-antibodies mainly recognize the second tandem repeat or the
C-terminus of ECM-1 (9). These domains also bind the extracellular matrix molecules fibulin-1 and perlecan (5, 10). The phenotypes of lipoid proteinosis and lichen sclerosis support a role for ECM-1 as a “biological glue” in the dermis (1).

  1. Chan, I. (2004) Exp. Dermatol. 29:52.
  2. Smits, P. et al. (1997) Genomics 45:487.
  3. Bhalerao, J. et al. (1995) J. Biol. Chem 270:16385.
  4. Smits, P. et al. (2000) J. Invest. Dermatol. 114:718.
  5. Mongiat, M. et al. (2003) J. Biol. Chem. 278:17491.
  6. Han, Z. et al. (2001) FASEB J. 15:988.
  7. Wang, L. et al. (2003) Cancer Lett. 200:57.
  8. Hamada, T. et al. (2003) J. Invest. Dermatol. 120:345.
  9. Oyama, N. et al. (2004) J. Clin. Invest. 113:1550.
  10. Fujimoto, N. et al. (2005) Biochem. Biophys. Res. Commun. 333:1327.
Long Name
Extracellular Matrix Protein 1
Entrez Gene IDs
1893 (Human); 13601 (Mouse); 116662 (Rat)
Alternate Names
ECM1; extracellular matrix protein 1; p85; Secretory Component Glycoprotein; Secretory Component P85

Citations for Recombinant Human ECM1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Extracellular matrix protein-1 secretory isoform promotes ovarian cancer through increasing alternative mRNA splicing and stemness
    Authors: H Yin, J Wang, H Li, Y Yu, X Wang, L Lu, C Lv, B Chang, W Jin, W Guo, C Ren, G Yang
    Nature Communications, 2021;12(1):4230.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Protective effect of stromal Dickkopf-3 in prostate cancer: opposing roles for TGFBI and ECM-1
    Authors: Z Al Shareef, H Kardooni, V Murillo-Ga, G Domenici, E Stylianaki, JH Steel, M Rabano, I Gorroño-Et, I Zabalza, M dM Vivanco, J Waxman, RM Kypta
    Oncogene, 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay


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