Recombinant Human EDA-A1/Ectodysplasin A1 Protein

Carrier Free

Catalog # Availability Size / Price Qty
3944-ED-010/CF

With Carrier

Catalog # Availability Size / Price Qty
3944-ED-010
R&D Systems Recombinant Proteins and Enzymes
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Citations (3)
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Recombinant Human EDA-A1/Ectodysplasin A1 Protein Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to compete with biotinylated human EDA-A1 for binding to immobilized rhEDA R/Fc Chimera.
Source
Mouse myeloma cell line, NS0-derived human EDA-A1/Ectodysplasin A1 protein
Ser160-Ser391 & Lys178-Ser391
Accession #
N-terminal Sequence
Analysis
Ser160 & Lys178
Structure / Form
Homotrimer
Predicted Molecular Mass
24.1 kDa & 22.2 kDa (monomers)
SDS-PAGE
30-40 kDa, reducing conditions

Product Datasheets

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3944-ED (with carrier)

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3944-ED/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3944-ED

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

3944-ED/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

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Background: EDA-A1/Ectodysplasin A1

Ectodysplasin is a 45 kDa type II transmembrane TNF superfamily protein that is associated with X-linked hypohidrotic ectodermal dysplasia (HED), a disorder of hair, tooth, and sweat gland development (1 - 4). The human EDA-A1 cDNA encodes a 41 amino acid (aa) cytoplasmic region, a 21 aa transmembrane segment, and a 329 aa extracellular region that contains a terminal TNF homology domain, a collagenous domain, and a stalk region (3, 5, 6). Within the collagenous and TNF homology domains, human EDA-A1 shares greater than 97% aa sequence identity with bovine, canine, mouse, and rat EDA-A1. Multiple alternately spliced EDA variants have been described (4, 7). The dominant variant, EDA-A2, has a deletion of two amino acids that changes the receptor binding selectivity from EDAR to XEDAR (4, 7, 8). The collagenous domain of EDA-A1 mediates non-covalent homotrimer formation (5, 6). Shedding of the collagenous and TNF homology domains of EDA-A1 is accomplished by a furin-like protease. The released fragment maintains its trimeric state and ability to bind EDAR (9, 10). Some EDA-A1 polymorphisms found in HED patients alter the protease recognition site and prevent shedding (9). EDA-A1 is expressed in developing hair follicles, epidermis, teeth, sweat glands, salivary glands, and forebrain (6, 8, 11 - 13). It regulates ectodermal appendage formation and is critical to the patterning and morphogenesis of hair follicles, partially through the induction of Lymphotoxin beta (5, 12, 14). Receptor and ligand expression are regulated by factors involved in many aspects of tissue morphogenesis. EDA-A1 expression is induced by Wnt6 (12, 13), while the expression of EDAR is induced by Activin beta A and inhibited by BMP-2, -4, and -7 (13, 15).

References
  1. Mikkola, M.L. and I. Thesleff (2003) Cytokine Growth Factor Rev. 14:211. 
  2. Botchkarev, V.A. and M.Y. Fessing (2005) J. Invest. Dermatol. 10:247.  
  3. Kere, J. et al. (1996) Nat. Genet. 13:409. 
  4. Bayes, M. et al. (1998) Hum. Mol. Genet. 7:1661. 
  5. Ezer, S. et al. (1999) Hum. Mol. Genet. 8:2079.  
  6. Mikkola, M.L. et al. (1999) Mech. Dev. 88:133. 
  7. Hashimoto, T. et al. (2006) Gene 371:42. 
  8. Yan, M. et al. (2000) Science 290:523.
  9. Chen, Y. et al. (2001) Proc. Natl. Acad. Sci. 98:7218.
  10. Elomaa, O. et al. (2001) Hum. Mol. Genet. 10:953.
  11. Pispa, J. et al. (2003) Gene Exp. Patterns 3:675.
  12. Laurikkala, J. et al. (2002) Development 129:2541.
  13. Laurikkala, J. et al. (2001) Dev. Biol. 229:443.
  14. Cui, C.Y. et al. (2006) Proc. Natl. Acad. Sci. 103:9142.
  15. Mou, C. et al. (2006) Proc. Natl. Acad. Sci. 103:9075.
Entrez Gene IDs
1896 (Human); 13607 (Mouse)
Alternate Names
ECTD1;Ectodysplasin-A;ED1;ED1-A1;ED1-A2;EDA1;EDA2;EDA-A1;EDA-A2;HED;HED1;ODT1;STHAGX1;TNLG7C;XHED;XLHED; EDAA1; EDA-A1

Citations for Recombinant Human EDA-A1/Ectodysplasin A1 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. EDA2R-NIK signalling promotes muscle atrophy linked to cancer cachexia
    Authors: Bilgic, SN;Domaniku, A;Toledo, B;Agca, S;Weber, BZC;Arabaci, DH;Ozornek, Z;Lause, P;Thissen, JP;Loumaye, A;Kir, S;
    Nature
    Species: Mouse
    Sample Types: Transduced Whole Cells, Whole Cells
    Applications: Bioassay
  2. An anti-TL1A antibody for the treatment of asthma and inflammatory bowel disease
    Authors: AW Clarke, L Poulton, D Shim, D Mabon, D Butt, M Pollard, V Pande, J Husten, J Lyons, C Tian, AG Doyle
    MAbs, 2018-03-05;0(0):1-43.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance (SPR
  3. Ectodysplasin A protein promotes corneal epithelial cell proliferation
    Authors: S Li, J Zhou, J Bu, K Ning, L Zhang, J Li, Y Guo, X He, H He, X Cai, Y Chen, PS Reinach, Z Liu, W Li
    J. Biol. Chem., 2017-06-27;292(32):13391-13401.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo

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