Recombinant Human EGF-L6 Protein, CF

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R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human EGF-L6 Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by the ability of the immobilized protein to support the adhesion of NIH‑3T3 mouse embryonic fibroblast cells. Osada, A. et al. (2005) Exp. Cell Res. 303:148. The ED50 for this effect is 1-5 μg/mL.
Human embryonic kidney cell, HEK293-derived human EGF-L6 protein
Human EGF-L6
Accession # Q8IUX8
N-terminus C-terminus
Accession #
N-terminal Sequence
Predicted Molecular Mass
40 kDa
40-55 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in MES and NaCl.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: EGF-L6

Epidermal Growth Factor-like Domain, Multiple 6 (EGF-L6), also known as MAM- and EGF-containing (MAEG) is a member of the EGF repeat superfamily of proteins. This secreted protein contains five EGF-like repeats, an RGD motif that binds to Integrin alpha 8 beta 1, and a C-terminal MAM domain (1, 2). Within shared regions, human EGF-L6 shares 75% and 78% amino acid sequence identity with the mouse and rat orthologs, respectively. EGF-L6 is expressed in a multitude of fetal tissues during early development such as lung, heart, liver, spleen, cochlea, and placenta (3, 4). It is also expressed in the dermatome and dermatome-derived tissues including trunk dermis, hair follicles, and cranio-facial mesenchyme (5). EGF-L6 is expressed by osteoblastic-like cells and has been shown to act in a paracrine manner to promote migration of vascular endothelial cells, thus regulating angiogenesis in the local environment of the bone (4, 6). Additionally, EGF-L6 is expressed by epidermal stem cells in the hair follicle bulge and is believed to play a role in regulating the dermal vasculature (7). EGF-L6 is also produced by mature adipocytes and is thought to be involved in the process of adipose tissue expansion and the development of obesity (8). EGF-L6 expression is increased in fibroblastic meningioma and ovarian cancer and has been proposed as a biomarker for the latter (9, 10).

  1. Buchner, G. et al. (2000) Genomics 65:16.
  2. Osada, A. et al. (2005) Exp. Cell Res. 303:148.
  3. Yeung, G. et al. (1999) Genomics 62:304.
  4. Chim, S.M. et al. (2011) J. Biol. Chem. 286:22035.
  5. Buchner, G. et al. (2000) Mech. Dev. 98:179.
  6. Chim, S.M. et al. (2013) Cytokine Growth Factor Rev. 24:297.
  7. Xiao, Y. et al. (2013) J. Invest. Dermatol. 133:2324.
  8. Oberauer, R. et al. (2010) Mol. Cell. Biochem. 343:257.
  9. Buckanovich, R.J. et al. (2007) J. Clin. Oncol. 25:852.
  10. Wang, X. et al. (2012) PLoS ONE 7:e52707.
Long Name
EGF-like Domain, Multiple 6
Entrez Gene IDs
25975 (Human); 54156 (Mouse); 317470 (Rat)
Alternate Names
DKFZp564P2063; EGF repeat-containing protein 6; EGFL6; EGF-L6; EGF-like protein 6; EGF-like-domain, multiple 6; epidermal growth factor-like protein 6; MAEG; MAM and EGF domain containing; MAM and EGF domains-containing gene protein; W80


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