Recombinant Human Elongin B/Elongin C/VHL/CUL2/RBX1, CF

Catalog # Availability Size / Price Qty
E3-655-025
VHL/ELOB/ELOC/CUL2/RBX1 Complex Protein Bioactivity
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Recombinant Human Elongin B/Elongin C/VHL/CUL2/RBX1, CF Summary

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Typical protein concentration for use in vitro will depend on experimental conditions.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human VHL/ELOB/ELOC/CUL2/RBX1 Complex protein
Predicted Molecular Mass
25 kDa (VHL), 13.1 kDa (ELOB), 12.8 kDa (ELOC), 93 kDa (CUL2), 12 kDa (RBX1)

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E3-655

Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol, 2 mM DTT
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 48 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Data Image

SDS-PAGE SDS-PAGE View Larger

1  μg and 2  μg per lane of Recombinant Human VHL/ELOB/ELOC/CUL2/RBX1 Complex (Catalog # E3-655) was resolved with SDS-PAGE under reducing conditions and visualized by colloidal Coomassie Blue staining, showing bands corresponding to the individual proteins of the complex.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VHL/ELOB/ELOC/CUL2/RBX1 Complex

VHL (von Hippel-Lindau disease tumor suppressor) is the substrate recognition subunit for an E3 ligase activity that ubiquitinates proteins containing hydroxyproline residues.  Targets of VHL include HIF1 alpha, beta 2 adrenergic receptor, ZHX2 and others.  In VHL-box E3 Ubiquitin ligases, VHL is bound to a heterodimer complex composed of Elongin B (ELOB) and Elongin C (ELOC) through the "BC-box motif" found in many proteins in the VHL-box and SOCS-box protein families.  ELOB/ELOC serves as an adapter between substrate recognition proteins and Cullin-2 (or Cullin-5) and RBX1 to form an active E3 ligase.  This protein complex contains an amino-terminal His-tag on the CUL2 subunit.

References
  1. Kamura T., et al. (1998) Genes & Dev.  doi: 10.1101/gad.12.24.3872
  2. Okumura F., et al. (2012) Front. Oncol.  doi: 10.3389/fonc.2012.00010
  3. Xie L., et al. (2009) Sci. Signaling  doi: 10.1126/scisignal.2000444
  4. Zhang J., et al. (2018) Science  doi: 10.1126/science.aap8411
Entrez Gene IDs
7428 (Human); 22346 (Mouse)
Alternate Names
VHL/ELOB/ELOC/CUL2/RBX1 Complex

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