Recombinant Human Endosialin/CD248 Protein, CF
Recombinant Human Endosialin/CD248 Protein, CF Summary
Gln18-Arg685, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Endosialin, also known as TEM1 (tumor endothelial marker 1) and designated CD248, is a 165‑175 kDa type I transmembrane O‑glycosylated protein that is expressed on activated perivascular and stromal cells in embyronic and tumor neovasculature, but down‑regulated in quiescent vasculature (1‑4). The human Endosialin cDNA encodes 757 amino acids (aa) including a 17 aa signal sequence, a 670 aa extracellular domain (ECD) with one C‑type lectin, one Sushi, one EGF‑like domain and a mucin-like stalk, a transmembrane sequence, and a short (49 aa) cytoplasmic domain with three potential phosphorylation sites and a PDZ binding motif (2, 3). Within the ECD, human Endosialin shares 76% and 74% aa sequence identity with mouse and rat Endosialin, respectively. Endosialin regulates pericyte proliferation, migration, and adhesion to endothelial cell‑deposited fibronectin and collagens I and IV in the extracellular matrix (3‑7). It binds Galectin‑3/MAC‑2BP, mediating a repulsive signal thought to guide tumor cell migration (5). In blood vessel development, it promotes endothelial cell apoptosis and pruning of unwanted capillaries (6). In humans, it is up‑regulated on mesenchymal stem cells, naïve CD8+ T cells, and leukocytes that express markers of endothelial and dendritic lineages (3, 8). It is dynamically expressed on lymphoid tissue stromal cells and is required for lymph node, spleen, and thymus remodeling during immune responses (8‑11). Its expression correlates with suppressed proliferation of human naïve CD8+ T cells, and with aggressiveness of some tumors (8, 12, 13). Endosialin expression is up‑regulated in vitro by high cell density and hypoxia (3, 14). Inflammatory conditions such as rheumatoid and psoriatic arthritis and IgA nephropathy can cause expression in stroma that do not normally express Endosialin (3).
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Citations for Recombinant Human Endosialin/CD248 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Targeting fibroblast CD248 attenuates CCL17-expressing macrophages and tissue fibrosis
Authors: CH Pai, SR Lin, CH Liu, SY Pan, H Hsu, YT Chen, CT Yen, IS Yu, HL Wu, SL Lin, SW Lin
Sci Rep, 2020-10-08;10(1):16772.
Sample Types: Whole Cells
Generation of a novel Antibody-Drug Conjugate targeting endosialin: potent and durable antitumor response in sarcoma
Authors: E Capone, E Piccolo, I Fichera, P Ciufici, D Barcaroli, A Sala, V De Laurenz, V Iacobelli, S Iacobelli, G Sala
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