Recombinant Human EPCR Protein, CF Summary
Ser18-Ser210, with a C-terminal 10-His tag
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CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
When human Activated Protein C is immobilized at 3 μg/mL, 100 μL/well, Recombinant Human EPCR (Catalog # 9557-ER) binds with an ED50 of 1-5 μg/mL.
The endothelial protein C receptor (EPCR), also known as CD201, is a transmembrane glycoprotein expressed on vascular endothelial cells and functions as a negative regulator of thrombosis (1). Mature human EPCR consists of a 193 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 7 aa cytoplasmic tail (2). Within the ECD, human EPCR shares 63% and 66% aa sequence identity with mouse and rat EPCR, respectively. EPCR inhibits thrombosis through its interactions with Protein C, activated Protein C (APC), and Coagulation Factors VII, and VIIa (3, 4). It enhances the activation of Protein C in response to complexes of Thrombin-Thrombomodulin (5). In humans, a soluble form of EPCR can be produced by alternative splicing or ADAM17/TACE mediated shedding (6-9), and this protein inhibits the anti-coagulant activity of APC (10, 11). EPCR can be degraded on the surface of endothelial cells by Neutrophil Elastase (12). Activation of EPCR also protects vascular endothelial cells from Thrombin-induced apoptosis (13). EPCR binds to CD11b/CD18 (Mac-1) on monocytes and mediates monocyte adhesion to the vascular endothelium (14). In addition, EPCR binds to the antigen receptor on gamma δ T cells (15), promotes hematopoietic stem cell retention in the bone marrow (9), and binds to surface proteins of some species of Plasmodium, contributing to pathogenicity in severe malaria (16).
- Montes, R. et al. (2012) Thromb. Haemost. 107:815.
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- Stearns-Kurosawa, D.J. et al. (1996) Proc. Natl. Acad. Sci. USA 93:10212.
- Saposnik, B. et al. (2008) Blood 111:3442.
- Qu, D. et al. (2007) J. Thromb. Haemost. 5:395.
- Xu, J. et al. (2000) J. Biol. Chem. 275:6038.
- Gur-Cohen, S. et al. (2015) Nat. Med. 21:1307.
- Kurosawa, S. et al. (1997) J. Clin. Invest. 100:411.
- Liaw, P.C. et al. (2000) J. Biol. Chem. 275:5447.
- Villegas-Mendez, A. et al. (2007) J. Thromb. Haemost. 5:980.
- Bae, J.-S. et al. (2007) Blood 110:3909.
- Fink, K. et al. (2013) PLoS One 8:e53103.
- Willcox, C.R. et al. (2012) Nat. Immunol. 13:872.
- Turner, L. et al. (2013) Nature 498:502.
Citation for Recombinant Human EPCR Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Elucidating mechanisms of genetic cross-disease associations at the PROCR vascular disease locus
Authors: D Stacey, L Chen, PJ Stanczyk, JMM Howson, AM Mason, S Burgess, S MacDonald, J Langdown, H McKinney, K Downes, N Farahi, JE Peters, S Basu, JS Pankow, W Tang, N Pankratz, M Sabater-Ll, PS de Vries, NL Smith, CHARGE Hem, AD Gelinas, DJ Schneider, N Janjic, NJ Samani, S Ye, C Summers, ER Chilvers, J Danesh, DS Paul
Nature Communications, 2022;13(1):1222.
Sample Types: Whole Cells
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