Recombinant Human EphA2 Protein, CF

Catalog # Availability Size / Price Qty
3035-A2-100
Product Details
Citations (6)
FAQs
Supplemental Products
Reviews (1)

Recombinant Human EphA2 Protein, CF Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized rhEphA2 at 2 µg/mL (100 µL/well) can bind rmEphrin-A1/Fc Chimera with a linear range of 0.078-5 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human EphA2 protein
Gln25-Asn534, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
No results obtained: Gln25 predicted & Lys27
Predicted Molecular Mass
56.9 kDa
SDS-PAGE
70-75 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3035-A2

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EphA2

EphA2, also known as Eck, Myk2, and Sek2, is a member of the Eph receptor tyrosine kinase family which binds Ephrins A1, 2, 3, 4, and 5 (1, 2, 3, 4). A and B class Eph proteins have a common structural organization. The human EphA2 cDNA encodes a 976 amino acid (aa) precursor including a 24 aa signal sequence, a 510 aa extracellular domain (ECD), a 24 aa transmembrane segment, and a 418 aa cytoplasmic domain. The ECD contains an N-terminal globular domain, a cysteine-rich domain, and two fibronectin type III domains (5). The cytoplasmic domain contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a sterile alpha motif (SAM) (5). The ECD of human EphA2 shares 90 - 94% aa sequence identity with mouse, bovine, and canine EphA2, and approximately 45% aa sequence identity with human EphA1, 3, 4, 5, 7, and 8. EphA2 becomes autophosphorylated following ligand binding (6, 7) and then interacts with SH2 domain-containing PI3-kinase to activate MAPK pathways (8, 9). Reverse signaling is also propagated through the Ephrin ligand. Transcription of EphA2 is dependent on the expression of E-Cadherin (10), and can be induced by p53 family transcription factors (11). EphA2 is upregulated in breast, prostate, and colon cancer vascular endothelium. Its ligand, EphrinA1, is expressed by the local tumor cells (12, 13). In some cases, EphA2 and EphrinA1 are expressed on the same blood vessels (14). EphA2 signaling cooperates with VEGF receptor signaling in promoting endothelial cell migration (13). The gene encoding human EphA2 maps to a region on chromosome 1 which is frequently deleted in neuroectodermal tumors (15).

References
  1. Poliakov, A. et al. (2004) Dev. Cell 7:465.
  2. Surawska, H. et al. (2004) Cytokine Growth Factor Rev. 15:419. 
  3. Pasquale, E.B. (2005) Nat. Rev. Mol. Cell Biol. 6:462.
  4. Davy, A. and P. Soriano (2005) Dev. Dyn. 232:1.
  5. Bohme, B et al. (1993) Oncogene 8:2857.
  6. Pandey, A. et al. (1995) Science 268:567.
  7. Bartley, T.D. et al. (1994) Nature 368:558.
  8. Pandey, A. et al. (1994) J. Biol. Chem. 269:30154.
  9. Miao, H. et al. (2001) Nat. Cell Biol. 3:527.
  10. Orsulic, S. and R. Kemler (2000) J. Cell Sci. 113:1793.
  11. Dohn, M. et al. (2001) Oncogene 20:6503.
  12. Zelinski, D.P. et al. (2001) Cancer Res. 61:2301.
  13. Brantley, D.M. et al. (2002) Oncogene 21:7011.
  14. Ogawa, K. et al. (2000) Oncogene 19:6043.
  15. Sulman, E.P. et al. (1997) Genomics 40:371.
Entrez Gene IDs
1969 (Human); 13836 (Mouse)
Alternate Names
ARCC2; EC 2.7.10; EC 2.7.10.1; Eck; ECKepithelial cell receptor protein tyrosine kinase; EPH receptor A2; EphA2; ephrin type-A receptor 2; Epithelial cell kinase; Myk2; Sek2; soluble EPHA2 variant 1; Tyrosine-protein kinase receptor ECK

Citations for Recombinant Human EphA2 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Small extracellular vesicles secreted from senescent cells promote cancer cell proliferation through EphA2
    Authors: M Takasugi, R Okada, A Takahashi, D Virya Chen, S Watanabe, E Hara
    Nat Commun, 2017;8(0):15729.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. EphA receptors and ephrin-A ligands are upregulated by monocytic differentiation/maturation and promote cell adhesion and protrusion formation in HL60 monocytes
    Authors: M Mukai, N Suruga, N Saeki, K Ogawa
    BMC Cell Biol., 2017;18(1):28.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Quantitative assessment of antibody internalization with novel monoclonal antibodies against Alexa fluorophores.
    Authors: Liao-Chan, Sindy, Daine-Matsuoka, Barbara, Heald, Nathan, Wong, Tiffany, Lin, Tracey, Cai, Allen G, Lai, Michelle, D'Alessio, Joseph A, Theunissen, Jan-Will
    PLoS ONE, 2015;10(4):e0124708.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Isolation and Characterization of a Monobody with a Fibronectin Domain III Scaffold That Specifically Binds EphA2.
    Authors: Park S, Park S, Kim D, Pyo A, Kimura R, Sathirachinda A, Choy H, Min J, Gambhir S, Hong Y
    PLoS ONE, 2015;10(7):e0132976.
    Species: Yeast
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. The ephrin receptor tyrosine kinase A2 is a cellular receptor for Kaposi's sarcoma-associated herpesvirus.
    Authors: Hahn AS, Kaufmann JK, Wies E, Naschberger E, Panteleev-Ivlev J, Schmidt K, Holzer A, Schmidt M, Chen J, Konig S, Ensser A, Myoung J, Brockmeyer NH, Sturzl M, Fleckenstein B, Neipel F
    Nat. Med., 2012;18(6):961-6.
    Species: Human
    Sample Types: In Vivo
    Applications: In Vivo
  6. A human antibody-drug conjugate targeting EphA2 inhibits tumor growth in vivo.
    Authors: Jackson D, Gooya J, Mao S, Kinneer K, Xu L, Camara M, Fazenbaker C, Fleming R, Swamynathan S, Meyer D, Senter PD, Gao C, Wu H, Kinch M, Coats S, Kiener PA, Tice DA
    Cancer Res., 2008;68(22):9367-74.
    Species: N/A
    Sample Types: N/A
    Applications: Binding Assay

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Recombinant Human EphA2 Protein, CF
By Anonymous on 01/29/2019
Application: Immunoassay Standard