Recombinant Human EphA5 Protein, CF

R&D Systems | Catalog # 3036-A5

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Key Product Details

  • R&D Systems NS0-derived Recombinant Human EphA5 Protein (3036-A5)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

AAI43428

Applications

Binding Activity
View all EphA5 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human EphA5 protein
Ser56-Pro573, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser56

Predicted Molecular Mass

58.4 kDa

SDS-PAGE

75-85 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Immobilized Recombinant Human EphA5 at 2 µg/mL (100 µL/well) can bind recombinant mouse Ephrin-A2 Fc Chimera with a linear range of 0.16-10 ng/mL.

Formulation, Preparation, and Storage

3036-A5
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EphA5

EphA5, also known as Ehk1, Bsk, Cek7, Hek7, and Rek7, is a member of the Eph receptor tyrosine kinase family which binds members of the Ephrin ligand family (1‑4). The A and B class Eph proteins have a common structural organization. Activation of kinase activity occurs after membrane-bound or clustered ligand recognition and binding. Reverse signaling is propagated through the Ephrin ligand. The human EphA5 cDNA encodes a 1037 amino acid (aa) precursor including a 24 aa signal sequence, a 549 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 443 aa cytoplasmic domain (5). The ECD contains an N-terminal globular domain, a cysteine-rich domain, and two fibronectin type III domains. The cytoplasmic domain contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) (5). Multiple splice variants of human EphA5 are produced in brain and differ in their extracellular and cytoplasmic sequences (6). Taking into account differences in splice variants between species, comparable regions of the human EphA5 ECD share greater than 96% aa sequence identity with bovine, mouse, and rat EphA5, approximately 40% aa identity with human EphA1 and EphA2, and 54‑61% aa identity with human EphA3, 4, 7, and 8. EphA5 is widely expressed in neurons of the adult human CNS (6), with the exception of few areas such as the substantia nigra (7). In rat, the expression of EphA5 during embryogenesis suggests a role in the development of CNS neurons (8).

References

  1. Poliakov, A. et al. (2004) Dev. Cell 7:465.
  2. Surawska, H. et al. (2004) Cytokine Growth Factor Rev 15:419.
  3. Pasquale, E.B. (2005) Nat. Rev. Mol. Cell Biol. 6:462.
  4. Davy, A. and P. Soriano (2005) Dev. Dyn. 232:1.
  5. Fox, G.M. et al. (1995) Oncogene 10:897.
  6. Miescher, G.C. et al. (1997) Mol. Brain Res. 46:17.
  7. Olivieri, G. and G.C. Miescher (1999) J. Histochem. Cytochem. 47:855.
  8. Taylor, V. et al. (1994) Neuroscience 63:163.

Alternate Names

Bsk, Cek7, Ehk1, Hek7, Rek7

Entrez Gene IDs

2044 (Human); 13839 (Mouse); 79208 (Rat)

Gene Symbol

EPHA5

UniProt

AAI43428

Additional EphA5 Products

Product Documents for Recombinant Human EphA5 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human EphA5 Protein, CF

For research use only

Citations for Recombinant Human EphA5 Protein, CF

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