Recombinant Human EphA5 Protein, CF
Recombinant Human EphA5 Protein, CF Summary
Ser56-Pro573, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
EphA5, also known as Ehk1, Bsk, Cek7, Hek7, and Rek7, is a member of the Eph receptor tyrosine kinase family which binds members of the Ephrin ligand family (1 - 4). The A and B class Eph proteins have a common structural organization. Activation of kinase activity occurs after membrane-bound or clustered ligand recognition and binding. Reverse signaling is propagated through the Ephrin ligand. The human EphA5 cDNA encodes a 1037 amino acid (aa) precursor including a 24 aa signal sequence, a 549 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 443 aa cytoplasmic domain (5). The ECD contains an N-terminal globular domain, a cysteine-rich domain, and two fibronectin type III domains. The cytoplasmic domain contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) (5). Multiple splice variants of human EphA5 are produced in brain and differ in their extracellular and cytoplasmic sequences (6). Taking into account differences in splice variants between species, comparable regions of the human EphA5 ECD share greater than 96% aa sequence identity with bovine, mouse, and rat EphA5, approximately 40% aa identity with human EphA1 and EphA2, and 54 - 61% aa identity with human EphA3, 4, 7, and 8. EphA5 is widely expressed in neurons of the adult human CNS (6), with the exception of few areas such as the substantia nigra (7). In rat, the expression of EphA5 during embryogenesis suggests a role in the development of CNS neurons (8).
- Poliakov, A. et al. (2004) Dev. Cell 7:465.
- Surawska, H. et al. (2004) Cytokine Growth Factor Rev 15:419.
- Pasquale, E.B. (2005) Nat. Rev. Mol. Cell Biol. 6:462.
- Davy, A. and P. Soriano (2005) Dev. Dyn. 232:1.
- Fox, G.M. et al. (1995) Oncogene 10:897.
- Miescher, G.C. et al. (1997) Mol. Brain Res. 46:17.
- Olivieri, G. and G.C. Miescher (1999) J. Histochem. Cytochem. 47:855.
- Taylor, V. et al. (1994) Neuroscience 63:163.
Citations for Recombinant Human EphA5 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
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Selection of phage-displayed accessible recombinant targeted antibodies (SPARTA): methodology and applications
Authors: S D'Angelo, FI Staquicini, F Ferrara, DI Staquicini, G Sharma, CA Tarleton, H Nguyen, LA Naranjo, RL Sidman, W Arap, AR Bradbury, R Pasqualini
JCI Insight, 2018;3(9):.
Sample Types: Phage Antibody Library
Receptor tyrosine kinase EphA5 is a functional molecular target in human lung cancer.
Authors: Staquicini F, Qian M, Salameh A, Dobroff A, Edwards J, Cimino D, Moeller B, Kelly P, Nunez M, Tang X, Liu D, Lee J, Hong W, Ferrara F, Bradbury A, Lobb R, Edelman M, Sidman R, Wistuba I, Arap W, Pasqualini R
J Biol Chem, 2015;290(12):7345-59.
Sample Types: In Vivo
The ephrin receptor tyrosine kinase A2 is a cellular receptor for Kaposi's sarcoma-associated herpesvirus.
Authors: Hahn AS, Kaufmann JK, Wies E, Naschberger E, Panteleev-Ivlev J, Schmidt K, Holzer A, Schmidt M, Chen J, Konig S, Ensser A, Myoung J, Brockmeyer NH, Sturzl M, Fleckenstein B, Neipel F
Nat. Med., 2012;18(6):961-6.
Sample Types: Whole Cells
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