Recombinant Human EphA6 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
5606-A6-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human EphA6 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When rhEphA6/Fc Chimera is immobilized at 2 μg/mL, 100 μL/well, it binds biotinylated rhEphrin-A3/Fc Chimera with a linear range of
0.31-20 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human EphA6 protein
Human EphA6
(Trp23-Val550)
Accession # AAI56734
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Trp23
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
85.7 kDa (monomer)
SDS-PAGE
95-110 kDa under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5606-A6

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Reconstitution Calculator

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Background: EphA6

EphA6, also known as Ehk2 and Hek12, is a 110 kDa member of the transmembrane Eph receptor tyrosine kinase family. The A and B classes of Eph proteins are distinguished by Ephrin ligand binding preference but have a common structural organization. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression (1 - 3). The 527 amino acid (aa) extracellular domain (ECD) of human EphA6 contains an N-terminal Ephrin binding region, a cysteine-rich region, and two fibronectin type II domains. The 465 aa cytoplasmic domain contains the tyrosine kinase domain and a sterile alpha motif (SAM) (4, 5). Alternate splicing generates an isoform that consists of the transmembrane segment and a portion of the kinase domain. Within the ECD, human EphA6 shares 95% aa sequence identity with mouse and rat EphA6. EphA6 binds and is activated by all five of the Ephrin-A ligands (6). It is primarily expressed in the brain and testes with lesser amounts in the colon and inner ear (4, 5, 7). Within the developing brain, it is localized to deep layers of the posterior cortical plate (8, 9). In adult rodents, EphA6 is expressed on Purkinje cells, in the accessory olfactory bulb, and on vestibular ganglion neurons of the inner ear (10, 11). This pattern is complementary to the expression of its ligand, Ephrin-A5 (9, 11). EphA6 null mice exhibit defects in learning and memory formation (12). EphA6 expression in vascular endothelium of the genital tubercle is under the regulation of HOX13, and a lack of HOX13 leads to hypospadias in mice (13). EphA6 is downregulated in human colon and renal cancers (7).

References
  1. Pasquale, E.B. (2008) Cell 133:38.
  2. Merlos-Suarez, A. and E. Batlle (2008) Curr. Opin. Cell Biol. 20:194.
  3. Pasquale, E.B. (2005) Nat. Rev. Mol. Cell Biol. 6:462.
  4. Lee, A.M. et al. (1996) DNA Cell Biol. 15:817.
  5. Maisonpierre, P.C. et al. (1993) Oncogene 8:3277.
  6. Gale, N.W. et al. (1996) Neuron 17:9.
  7. Hafner, C. et al. (2004) Clin. Chem. 50:490.
  8. Donoghue, M.J. and P. Rakic (1999) J. Neurosci. 19:5967.
  9. Yun, M.E. et al. (2003) J. Comp. Neurol. 456:203.
  10. Matsunaga, T. et al. (2000) Eur. J. Neurosci. 12:1599.
  11. Knoll, B. et al. (2001) Development 128:895.
  12. Savelieva, K.V. et al. (2008) Neurosci. Lett. 438:205.
  13. Shaut, C.A. et al. (2007) Dev. Dyn. 236:951.
Long Name
Eph Receptor A6
Entrez Gene IDs
285220 (Human); 13840 (Mouse)
Alternate Names
DKFZp434C1418; EC 2.7.10; EC 2.7.10.1; Ehk2; EHK-2; EPA6; EPH homology kinase 2; EPH receptor A6; EphA6; ephrin receptor EphA6; ephrin type-A receptor 6; FLJ35246; Hek12; PRO57066

Citation for Recombinant Human EphA6 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Ephrin receptor A10 monoclonal antibodies and the derived chimeric antigen receptor T cells exert an antitumor response in mouse models of triple-negative breast cancer
    Authors: JH Cha, LC Chan, YN Wang, YY Chu, CH Wang, HH Lee, W Xia, WC Shyu, SP Liu, J Yao, CW Chang, FR Cheng, J Liu, SO Lim, JL Hsu, WH Yang, GN Hortobagyi, C Lin, L Yang, D Yu, LB Jeng, MC Hung
    The Journal of Biological Chemistry, 2022;0(0):101817.
    Species: N/A
    Sample Types: Protein
    Applications: ELISA Capture

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