Recombinant Human Ephrin-A5 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
374-EA-200
R&D Systems Recombinant Proteins and Enzymes
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Citations (31)
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Recombinant Human Ephrin-A5 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to compete with Biotinylated Recombinant Human Ephrin‑A5 Fc Chimera (Catalog # BT374) for binding to immobilized recombinant mouse Eph-A3 Fc Chimera in a functional ELISA assay.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived human Ephrin-A5 protein
Human Ephrin-A5
(Gln21-Asn203)
Accession # P52803
IEGRMD Human IgG1
(Pro100-Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
No results obtained: Gln21 predicted
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
48.6 kDa (monomer)
SDS-PAGE
50-55 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

374-EA

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Reconstitution Calculator

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Background: Ephrin-A5

Ephrin‑A5, also known as AL‑1, RAGS, and LERK‑7, is an approximately 25 kDa member of the Ephrin‑A family of GPI‑anchored ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. Ephrin‑A ligands are structurally related to the extracellular domains of the transmembrane Ephrin‑B ligands. Eph‑Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression (1, 2). Ephrin‑A5 preferentially interacts with receptors in the EphA family but also with EphB2 (3). Mature human Ephrin‑A5 shares 99.5% aa sequence identity with mouse and rat Ephrin‑A5, respectively (4). Ephrin‑A5 is expressed in multiple tissues during development, particularly in the brain (5, 6). It can exert repulsive or attractive effects on migrating neurons in the developing brain and motor column of the spinal cord (6‑10). Ephrin‑A5 repels migrating axons by inducing growth cone collapse and neurite retraction and by inhibiting the neurotrophic effects of NGF and BDNF (3, 11, 12). It interacts in cis with EphA3 on retinal axon growth cones which reduces axonal sensitivity to Ephrin‑A5 in trans (13). In the adult, Ephrin‑A5 is expressed on hippocampal neurons and astrocytes and induces the development of hippocampal synapses (9, 14, 15). It supports the proliferation of neural progenitors and the survival of newly differentiated neurons (14). Ephrin‑A5 also functions as a tumor suppressor (16). Its normal function in inhibiting EGFR signaling is compromised by its down‑regulation in glioma (16). Ephrin‑A5 is also expressed by muscle precursor cells and interacts with EphA4 to restrict their migration to the correct locations during forelimb morphogenesis (17).

References
  1. Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.
  2. Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.
  3. Himanen, J.-P. et al. (2004) Nat. Neurosci. 7:501.
  4. Winslow, J.W. et al. (1995) Neuron 14:973.
  5. Deschamps, C. et al. (2010) BMC Neurosci. 11:105.
  6. Cooper, M.A. et al. (2009) Dev. Neurobiol. 69:36.
  7. Frisen, J. et al. (1998) Neuron 20:235.
  8. Zimmer, G. et al. (2008) Eur. J. Neurosci. 28:62.
  9. Otal, R. et al. (2006) Neuroscience 141:109.
  10. Eberhart, J. et al. (2004) J. Neurosci. 24:1070.
  11. Munoz, L.M. et al. (2005) Dev. Biol. 283:397.
  12. Meier, C. et al. (2011) PLoS ONE 6:e26089.
  13. Carvalho, R.F. et al. (2006) Nat. Neurosci. 9:322.
  14. Hara, Y. et al. (2010) Stem Cells 28:974.
  15. Akaneya, Y. et al. (2010) PLoS ONE 5:e12486.
  16. Li, J.-J. et al. (2009) Oncogene 28:1759.
  17. Swartz, M.E. et al. (2001) Development 128:4669.
Entrez Gene IDs
1946 (Human); 13640 (Mouse)
Alternate Names
AF1; AL-1; EFL-5; EFNA5; EPH-related receptor tyrosine kinase ligand 7; EphrinA5; Ephrin-A5; EPLG7; GLC1M; LERK-7; LERK7EFL5; RAGS

Citations for Recombinant Human Ephrin-A5 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

31 Citations: Showing 1 - 10
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  1. Giant ankyrin-B mediates transduction of axon guidance and collateral branch pruning factor sema 3A
    Authors: BA Creighton, S Afriyie, D Ajit, CR Casingal, KM Voos, J Reger, AM Burch, E Dyne, J Bay, JK Huang, ES Anton, MM Fu, DN Lorenzo
    Elife, 2021;10(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Ephrin A4-ephrin receptor A10 signaling promotes cell migration and spheroid formation by upregulating NANOG expression in oral squamous cell carcinoma cells
    Authors: YL Chen, YC Yen, CW Jang, SH Wang, HT Huang, CH Chen, JR Hsiao, JY Chang, YW Chen
    Scientific Reports, 2021;11(1):644.
    Species: Human
    Sample Types: Reference Standard
  3. A cancer mutation promotes EphA4 oligomerization and signaling by altering the conformation of the SAM domain
    Authors: TP Light, M Gomez-Sole, Z Wang, K Karl, E Zapata-Mer, MP Gehring, BC Lechtenber, TV Pogorelov, K Hristova, EB Pasquale
    The Journal of Biological Chemistry, 2021;297(1):100876.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Aggressive and recurrent ovarian cancers upregulate ephrinA5, a non-canonical effector of EphA2 signaling duality
    Authors: J Jukonen, L Moyano-Gal, K Höpfner, EA Pietilä, L Lehtinen, K Huhtinen, E Gucciardo, J Hynninen, S Hietanen, S Grénman, PM Ojala, O Carpén, K Lehti
    Scientific Reports, 2021;11(1):8856.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Replicating infant-specific reactive astrocyte functions in the injured adult brain
    Authors: L Teo, AG Boghdadi, J Homman-Lud, IC Mundinano, WC Kwan, JA Bourne
    Progress in neurobiology, 2021;0(0):102108.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  6. Spatial�organization-dependent EphA2 transcriptional responses revealed by ligand nanocalipers
    Authors: T Verheyen, T Fang, D Lindenhofe, Y Wang, K Akopyan, A Lindqvist, B Högberg, AI Teixeira
    Nucleic Acids Res., 2020;0(0):.
    Applications: Conjugation
  7. Structural and functional analyses reveal promiscuous and species specific use of ephrin receptors by Cedar virus
    Authors: ED Laing, CK Navaratnar, S Cheliout D, SR Petzing, Y Xu, SL Sterling, GA Marsh, LF Wang, M Amaya, DB Nikolov, R Cattaneo, CC Broder, K Xu
    Proc. Natl. Acad. Sci. U.S.A., 2019;0(0):.
    Species: Virus - Henipavirus
    Sample Types: Recombinant Protein
    Applications: Coprecipitation Assay
  8. CCR10+ epithelial cells from idiopathic pulmonary fibrosis lungs drive remodeling
    Authors: DM Habiel, MS Espindola, IC Jones, AL Coelho, B Stripp, CM Hogaboam
    JCI Insight, 2018;3(16):.
  9. EphrinA5 Signaling Is Required for the Distinctive Targeting of Raphe Serotonin Neurons in the Forebrain
    Authors: T Teng, A Gaillard, A Muzerelle, P Gaspar
    eNeuro, 2017;4(1):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  10. The Neural Cell Adhesion Molecule NCAM Promotes Clustering and Activation of EphA3 Receptors in GABAergic Interneurons to Induce RhoA/ROCK-mediated Growth Cone Collapse
    Authors: Patricia F Maness
    J. Biol. Chem., 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Anks1a regulates COPII-mediated anterograde transport of receptor tyrosine kinases critical for tumorigenesis
    Nat Commun, 2016;7(0):12799.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Combinatorial analysis of developmental cues efficiently converts human pluripotent stem cells into multiple neuronal subtypes.
    Authors: Maury Y, Come J, Piskorowski R, Salah-Mohellibi N, Chevaleyre V, Peschanski M, Martinat C, Nedelec S
    Nat Biotechnol, 2015;33(1):89-96.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Thalamic afferents influence cortical progenitors via ephrin A5-EphA4 interactions
    Authors: K Gerstmann, D Pensold, J Symmank, M Khundadze, CA Hübner, J Bolz, G Zimmer
    Development, 2015;142(1):140-50.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Role of synaptic plasticity and EphA5-ephrinA5 interaction within the ventromedial hypothalamus in response to recurrent hypoglycemia.
    Authors: Szepietowska, Barbara, Horvath, Tamas L, Sherwin, Robert S
    Diabetes, 2014;63(3):1140-7.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  15. The composition of EphB2 clusters determines the strength in the cellular repulsion response.
    Authors: Schaupp A, Sabet O, Dudanova I, Ponserre M, Bastiaens P, Klein R
    J Cell Biol, 2014;204(3):409-22.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. EphA5-EphrinA5 interactions within the ventromedial hypothalamus influence counterregulatory hormone release and local glutamine/glutamate balance during hypoglycemia.
    Authors: Szepietowska B, Zhu W, Czyzyk J, Eid T, Sherwin R
    Diabetes, 2013;62(4):1282-8.
    Species: Rat
    Sample Types: In Vivo
  17. EphA/ephrin-A signaling is critically involved in region-specific apoptosis during early brain development.
    Authors: Park, E, Kim, Y, Noh, H, Lee, H, Yoo, S, Park, S
    Cell Death Differ, 2013;20(1):169-80.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Ephrin-A5/EphA4 signalling controls specific afferent targeting to cochlear hair cells.
    Authors: Defourny J, Poirrier A, Lallemend F, Mateo Sanchez S, Neef J, Vanderhaeghen P, Soriano E, Peuckert C, Kullander K, Fritzsch B, Nguyen L, Moonen G, Moser T, Malgrange B
    Nat Commun, 2013;4(0):1438.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Astrocyte-produced ephrins inhibit schwann cell migration via VAV2 signaling.
    Authors: Afshari FT, Kwok JC, Fawcett JW
    J. Neurosci., 2010;30(12):4246-55.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  21. Ephrins negatively regulate cell proliferation in the epidermis and hair follicle.
    Authors: Genander M, Holmberg J, Frisen J
    Stem Cells, 2010;28(7):1196-205.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  22. Integration of neuronal clones in the radial cortical columns by EphA and ephrin-A signalling.
    Authors: Torii M, Hashimoto-Torii K, Levitt P, Rakic P
    Nature, 2009;461(7263):524-8.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  23. p75(NTR) mediates ephrin-A reverse signaling required for axon repulsion and mapping.
    Authors: Lim YS, McLaughlin T, Sung TC, Santiago A, Lee KF, O'Leary DD
    Neuron, 2008;59(5):746-58.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  24. Ephrin-A5 acts as a repulsive cue for migrating cortical interneurons.
    Authors: Zimmer G, Garcez P, Rudolph J, Niehage R, Weth F, Lent R, Bolz J
    Eur. J. Neurosci., 2008;28(1):62-73.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Meltrin beta/ADAM19 interacting with EphA4 in developing neural cells participates in formation of the neuromuscular junction.
    Authors: Yumoto N, Wakatsuki S, Kurisaki T, Hara Y, Osumi N, Frisen J, Sehara-Fujisawa A
    PLoS ONE, 2008;3(10):e3322.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  26. Soluble monomeric EphrinA1 is released from tumor cells and is a functional ligand for the EphA2 receptor.
    Authors: Wykosky J, Palma E, Gibo DM
    Oncogene, 2008;27(58):7260-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  27. Neurite responses to ephrin-A5 modulated by BDNF: evidence for TrkB-EphA interactions.
    Authors: Fitzgerald M, Buckley A, Lukehurst SS, Dunlop SA, Beazley LD, Rodger J
    Biochem. Biophys. Res. Commun., 2008;374(4):625-30.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  28. EphA-Ephrin-A-mediated beta cell communication regulates insulin secretion from pancreatic islets.
    Authors: Konstantinova I, Nikolova G, Ohara-Imaizumi M, Meda P, Kucera T, Zarbalis K, Wurst W, Nagamatsu S, Lammert E
    Cell, 2007;129(2):359-70.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  29. Adam meets Eph: an ADAM substrate recognition module acts as a molecular switch for ephrin cleavage in trans.
    Authors: Janes PW, Saha N, Barton WA, Kolev MV, Wimmer-Kleikamp SH, Nievergall E, Blobel CP, Himanen JP, Lackmann M, Nikolov DB
    Cell, 2005;123(2):291-304.
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunoprecipitation
  30. Ephrin-A5 inhibits growth of embryonic sensory neurons.
    Authors: Munoz LM, Zayachkivsky A, Kunz RB, Hunt JM, Wang G, Scott SA
    Dev. Biol., 2005;283(2):397-408.
    Species: Chicken
    Sample Types: Whole Cells
    Applications: ICC
  31. Ephrin stimulation modulates T cell chemotaxis.
    Authors: Sharfe N, Freywald A, Toro A, Dadi H, Roifman C
    Eur. J. Immunol., 2002;32(12):3745-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Recombinant Human Ephrin-A5 Fc Chimera Protein, CF
By Anonymous on 11/26/2019
Application: In vitro bioactivity in cell culture