Recombinant Human Ephrin-A5 Fc Chimera Protein, CF

(20 citations)   
  • Purity
    >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to compete with Biotinylated Recombinant Human Ephrin‑A5 Fc Chimera (Catalog # BT374) for binding to immobilized recombinant mouse Eph-A3 Fc Chimera in a functional ELISA assay.
    Optimal dilutions should be determined by each laboratory for each application.
  • Source
    Mouse myeloma cell line, NS0-derived
    Human Ephrin-A5
    (Gln21-Asn203)
    Accession # P52803
    IEGRMD Human IgG1
    (Pro100-Lys330)
    6-His tag
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    No results obtained: Gln21 predicted
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    48.6 kDa (monomer)
  • SDS-PAGE
    50-55 kDa, reducing conditions
374-EA
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Ephrin-A5
Ephrin‑A5, also known as AL‑1, RAGS, and LERK‑7, is an approximately 25 kDa member of the Ephrin‑A family of GPI‑anchored ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. Ephrin‑A ligands are structurally related to the extracellular domains of the transmembrane Ephrin‑B ligands. Eph‑Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression (1, 2). Ephrin‑A5 preferentially interacts with receptors in the EphA family but also with EphB2 (3). Mature human Ephrin‑A5 shares 99.5% aa sequence identity with mouse and rat Ephrin‑A5, respectively (4). Ephrin‑A5 is expressed in multiple tissues during development, particularly in the brain (5, 6). It can exert repulsive or attractive effects on migrating neurons in the developing brain and motor column of the spinal cord (6‑10). Ephrin‑A5 repels migrating axons by inducing growth cone collapse and neurite retraction and by inhibiting the neurotrophic effects of NGF and BDNF (3, 11, 12). It interacts in cis with EphA3 on retinal axon growth cones which reduces axonal sensitivity to Ephrin‑A5 in trans (13). In the adult, Ephrin‑A5 is expressed on hippocampal neurons and astrocytes and induces the development of hippocampal synapses (9, 14, 15). It supports the proliferation of neural progenitors and the survival of newly differentiated neurons (14). Ephrin‑A5 also functions as a tumor suppressor (16). Its normal function in inhibiting EGFR signaling is compromised by its down‑regulation in glioma (16). Ephrin‑A5 is also expressed by muscle precursor cells and interacts with EphA4 to restrict their migration to the correct locations during forelimb morphogenesis (17).
  • References:
    1. Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.
    2. Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.
    3. Himanen, J.-P. et al. (2004) Nat. Neurosci. 7:501.
    4. Winslow, J.W. et al. (1995) Neuron 14:973.
    5. Deschamps, C. et al. (2010) BMC Neurosci. 11:105.
    6. Cooper, M.A. et al. (2009) Dev. Neurobiol. 69:36.
    7. Frisen, J. et al. (1998) Neuron 20:235.
    8. Zimmer, G. et al. (2008) Eur. J. Neurosci. 28:62.
    9. Otal, R. et al. (2006) Neuroscience 141:109.
    10. Eberhart, J. et al. (2004) J. Neurosci. 24:1070.
    11. Munoz, L.M. et al. (2005) Dev. Biol. 283:397.
    12. Meier, C. et al. (2011) PLoS ONE 6:e26089.
    13. Carvalho, R.F. et al. (2006) Nat. Neurosci. 9:322.
    14. Hara, Y. et al. (2010) Stem Cells 28:974.
    15. Akaneya, Y. et al. (2010) PLoS ONE 5:e12486.
    16. Li, J.-J. et al. (2009) Oncogene 28:1759.
    17. Swartz, M.E. et al. (2001) Development 128:4669.
  • Entrez Gene IDs:
    1946 (Human); 13640 (Mouse)
  • Alternate Names:
    AF1; AL-1; EFL-5; EFNA5; EPH-related receptor tyrosine kinase ligand 7; EphrinA5; Ephrin-A5; EPLG7; GLC1M; LERK-7; LERK7EFL5; RAGS
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Anks1a regulates COPII-mediated anterograde transport of receptor tyrosine kinases critical for tumorigenesis
    Nat Commun, 2016;7(0):12799.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  2. Combinatorial analysis of developmental cues efficiently converts human pluripotent stem cells into multiple neuronal subtypes.
    Authors: Maury Y, Come J, Piskorowski R, Salah-Mohellibi N, Chevaleyre V, Peschanski M, Martinat C, Nedelec S
    Nat Biotechnol, 2015;33(1):89-96.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  3. Role of synaptic plasticity and EphA5-ephrinA5 interaction within the ventromedial hypothalamus in response to recurrent hypoglycemia.
    Authors: Szepietowska, Barbara, Horvath, Tamas L, Sherwin, Robert S
    Diabetes, 2014;63(3):1140-7.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  4. The composition of EphB2 clusters determines the strength in the cellular repulsion response.
    Authors: Schaupp A, Sabet O, Dudanova I, Ponserre M, Bastiaens P, Klein R
    J Cell Biol, 2014;204(3):409-22.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  5. EphA5-EphrinA5 interactions within the ventromedial hypothalamus influence counterregulatory hormone release and local glutamine/glutamate balance during hypoglycemia.
    Authors: Szepietowska B, Zhu W, Czyzyk J, Eid T, Sherwin R
    Diabetes, 2013;62(4):1282-8.
    Species: Rat
    Sample Type: In Vivo
  6. Ephrin-A5/EphA4 signalling controls specific afferent targeting to cochlear hair cells.
    Authors: Defourny J, Poirrier A, Lallemend F, Mateo Sanchez S, Neef J, Vanderhaeghen P, Soriano E, Peuckert C, Kullander K, Fritzsch B, Nguyen L, Moonen G, Moser T, Malgrange B
    Nat Commun, 2013;4(0):1438.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  7. EphA/ephrin-A signaling is critically involved in region-specific apoptosis during early brain development.
    Authors: Park, E, Kim, Y, Noh, H, Lee, H, Yoo, S, Park, S
    Cell Death Differ, 2013;20(1):169-80.
    Species: Mouse
    Sample Type: whole cells
    Application: Bioassay
  8. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012;487(7408):505-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  9. Ephrins negatively regulate cell proliferation in the epidermis and hair follicle.
    Authors: Genander M, Holmberg J, Frisen J
    Stem Cells, 2010;28(7):1196-205.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  10. Astrocyte-produced ephrins inhibit schwann cell migration via VAV2 signaling.
    Authors: Afshari FT, Kwok JC, Fawcett JW
    J. Neurosci., 2010;30(12):4246-55.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  11. Integration of neuronal clones in the radial cortical columns by EphA and ephrin-A signalling.
    Authors: Torii M, Hashimoto-Torii K, Levitt P, Rakic P
    Nature, 2009;461(7263):524-8.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  12. Meltrin beta/ADAM19 interacting with EphA4 in developing neural cells participates in formation of the neuromuscular junction.
    Authors: Yumoto N, Wakatsuki S, Kurisaki T, Hara Y, Osumi N, Frisen J, Sehara-Fujisawa A
    PLoS ONE, 2008;3(10):e3322.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  13. Soluble monomeric EphrinA1 is released from tumor cells and is a functional ligand for the EphA2 receptor.
    Authors: Wykosky J, Palma E, Gibo DM
    Oncogene, 2008;27(58):7260-73.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  14. p75(NTR) mediates ephrin-A reverse signaling required for axon repulsion and mapping.
    Authors: Lim YS, McLaughlin T, Sung TC, Santiago A, Lee KF, O'Leary DD
    Neuron, 2008;59(5):746-58.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  15. Neurite responses to ephrin-A5 modulated by BDNF: evidence for TrkB-EphA interactions.
    Authors: Fitzgerald M, Buckley A, Lukehurst SS, Dunlop SA, Beazley LD, Rodger J
    Biochem. Biophys. Res. Commun., 2008;374(4):625-30.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  16. Ephrin-A5 acts as a repulsive cue for migrating cortical interneurons.
    Authors: Zimmer G, Garcez P, Rudolph J, Niehage R, Weth F, Lent R, Bolz J
    Eur. J. Neurosci., 2008;28(1):62-73.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  17. EphA-Ephrin-A-mediated beta cell communication regulates insulin secretion from pancreatic islets.
    Authors: Konstantinova I, Nikolova G, Ohara-Imaizumi M, Meda P, Kucera T, Zarbalis K, Wurst W, Nagamatsu S, Lammert E
    Cell, 2007;129(2):359-70.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  18. Adam meets Eph: an ADAM substrate recognition module acts as a molecular switch for ephrin cleavage in trans.
    Authors: Janes PW, Saha N, Barton WA, Kolev MV, Wimmer-Kleikamp SH, Nievergall E, Blobel CP, Himanen JP, Lackmann M, Nikolov DB
    Cell, 2005;123(2):291-304.
    Species: Human
    Sample Type: Whole Cells
    Application: IP
  19. Ephrin-A5 inhibits growth of embryonic sensory neurons.
    Authors: Munoz LM, Zayachkivsky A, Kunz RB, Hunt JM, Wang G, Scott SA
    Dev. Biol., 2005;283(2):397-408.
    Species: Chicken
    Sample Type: Whole Cells
    Application: ICC
  20. Ephrin stimulation modulates T cell chemotaxis.
    Authors: Sharfe N, Freywald A, Toro A, Dadi H, Roifman C
    Eur. J. Immunol., 2002;32(12):3745-55.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
Expand to show all 20 Citations
Primary Antibodies
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His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Antibody

WB, Simple Western, Flow, AP, CyTOF-ready MAB050 26  
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His Tag HRP-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag Biotinylated Antibody

WB BAM050 4  
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His Tag PE-conjugated Antibody

ICFlow IC050P 2  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
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Histidine-tagged Protein Purification Resin

IP999 1
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Recombinant Proteins
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Recombinant Human IgG1 Fc, CF

BA 110-HG 64
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Recombinant Human Ephrin-A5 Fc Chimera Biotinylated Protein

Bind BT374 1
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