Recombinant Human Ephrin-B2 Fc Chimera Protein, CF

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7397-EB-050
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Recombinant Human Ephrin-B2 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human EphB2 Fc Chimera (Catalog # 5189-B2) is coated at 2 μg/mL, Recombinant Human Ephrin‑B2 Fc Chimera binds with an apparent Kd <0.1 nM.
Also measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neuron.
Source
Mouse myeloma cell line, NS0-derived human Ephrin-B2 protein
Human Ephrin-B2
(Met1-Ala229)
Accession # P52799
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Lys26
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
48.8 kDa (monomer)
SDS-PAGE
58-66 kDa, reducing conditions

Product Datasheets

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7397-EB

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7397-EB

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 300 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

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Background: Ephrin-B2

Ephrin-B2, also known as Htk-L, ELF-2, LERK-5, and NLERK-1, is a 40 kDa member of the Ephrin-B family of transmembrane ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. The extracellular domains of Ephrin-B ligands are structurally related to GPI-anchored Ephrin-A ligands. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression. Ephrin-B2 preferentially interacts with receptors in the EphB family (1, 2). Mature human Ephrin-B2 consists of a 202 amino acid (aa) extracelluar domain (ECD), a 21 aa transmembrane segment, and an 83 aa cytoplasmic domain (3). Within the ECD, human Ephrin-B2 shares 97% aa sequence identity with mouse and rat Ephrin-B2. Ephrin-B2 is expressed presynaptically on neurons (4, 5). It promotes presynaptic development, EphB2 shedding, axonal growth cone collapse, and neurite repulsion, and also regulates inflammatory and neuropathic pain (4-6). Ephrin-B2 is expressed by vascular mural cells and arterial vascular and lymphatic endothelium (7, 8). It exerts proliferative and migratory effects on these cells during angiogenesis and lymphangiogenesis in part by regulating the signaling activity of VEGF R2 and VEGF R3 (7-9). Ephrin-B2 plays a role in the immune response by mediating monocyte extravasation and T cell costimulation (10, 11). It is up‑regulated in invasive cancers and promotes tumor cell migration, invasion, and tumor angiogenesis (12-14). It functions as a cellular entry receptor for Hendra and Nipah viruses (15). Ephrin-B2 is also important for the separation of the urinary and intestinal tracts during development (16).

References
  1. Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.
  2. Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.
  3. Cerretti, D.P. et al. (1995) Mol. Immunol. 32:1197.
  4. McClelland, A.C. et al. (2009) Proc. Natl. Acad. Sci. 106:20487.
  5. Zhao, J. et al. (2010) Mol. Pain 6:77.
  6. Lin, K.-T. et al. (2008) J. Biol. Chem. 283:28969.
  7. Foo, S.S. et al. (2006) Cell 124:161.
  8. Wang, Y. et al. (2010) Nature 465:483.
  9. Sawamiphak, S. et al. (2010) Nature 465:487.
  10. Pfaff, D. et al. (2008) J. Cell Sci. 121:3842.
  11. Yu, G. et al. (2003) J. Immunol. 171:106.
  12. Meyer, S. et al. (2005) Int. J. Oncol. 27:1197.
  13. Nakada, M. et al. (2010) Int. J. Cancer 126:1155.
  14. Liu, W. et al. (2004) Br. J. Cancer 90:1620.
  15. Bonaparte, M.I. et al. (2005) Proc. Natl. Acad. Sci. 102:10652.
  16. Dravis, C. et al. (2004) Dev. Biol. 271:272.
Entrez Gene IDs
1948 (Human); 13642 (Mouse); 30219 (Zebrafish)
Alternate Names
EFNB2; ELF-2; ephrin B2; EphrinB2; Ephrin-B2; EPLG5; HTKL; Htk-L; LERK5; LERK-5; NLERK-1

Citations for Recombinant Human Ephrin-B2 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Colorectal cancer-associated mutations impair EphB1 kinase function
    Authors: Kim, Y;Ahmed, S;Miller, WT;
    The Journal of biological chemistry
    Species: Human
    Sample Types: Transfected Whole Cells, Whole Cells
    Applications: Bioassay
  2. EPH receptor B2 stimulates human monocyte adhesion and migration independently of its EphrinB ligands
    Authors: D Vreeken, CS Bruikman, SML Cox, H Zhang, R Lalai, A Koudijs, AJ van Zonnev, GK Hovingh, JM van Gils
    J. Leukoc. Biol., 2020-04-26;0(0):.
    Species: Human
    Sample Types: Whole Cell
    Applications: Cell Culture
  3. Endothelial EphB4 maintains vascular integrity and transport function in adult heart
    Authors: G Luxán, J Stewen, N Díaz, K Kato, SK Maney, A Aravamudha, F Berkenfeld, N Nagelmann, HC Drexler, D Zeuschner, C Faber, H Schillers, S Hermann, J Wiseman, JM Vaquerizas, ME Pitulescu, RH Adams
    Elife, 2019-11-29;8(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  4. Diabetic Ephrin-B2-Stimulated Peripheral Blood Mononuclear Cells Enhance Poststroke Recovery in Mice
    Authors: R Hilal, M Poittevin, A Pasteur-Ro, A Cogo, G Mangin, M Chevauché, Y Ziat, J Vilar, JM Launay, JF Gautier, D Broquères-, BI Levy, T Merkulova-, N Kubis
    Stem Cells Int, 2018-03-15;2018(0):2431567.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. EphB4 forward signalling mediates angiogenesis caused by CCM3/PDCD10-ablation
    Authors: C You, K Zhao, P Dammann, K Keyvani, I Kreitschma, U Sure, Y Zhu
    J. Cell. Mol. Med., 2017-04-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Downregulation of proinflammatory cytokines in HTLV-1-infected T cells by Resveratrol
    J Exp Clin Cancer Res, 2016-07-22;35(1):118.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. EPHB4 kinase-inactivating mutations cause autosomal dominant lymphatic-related hydrops fetalis
    J Clin Invest, 2016-07-11;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Murine, but not human, ephrin-B2 can be efficiently cleaved by the serine protease kallikrein-4: implications for xenograft models of human prostate cancer.
    Authors: Lisle J, Mertens-Walker I, Stephens C, Stansfield S, Clements J, Herington A, Stephenson S
    Exp Cell Res, 2015-02-24;333(1):136-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. RASA1 functions in EPHB4 signaling pathway to suppress endothelial mTORC1 activity.
    Authors: Kawasaki, Jun, Aegerter, Sandrine, Fevurly, R Dawn, Mammoto, Akiko, Mammoto, Tadanori, Sahin, Mustafa, Mably, John D, Fishman, Steven J, Chan, Joanne
    J Clin Invest, 2014-05-16;124(6):2774-84.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. LF-15 & T7, synthetic peptides derived from tumstatin, attenuate aspects of airway remodelling in a murine model of chronic OVA-induced allergic airway disease.
    Authors: Grafton K, Moir L, Black J, Hansbro N, Hansbro P, Burgess J, Oliver B
    PLoS ONE, 2014-01-15;9(1):e85655.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Unraveling a three-step spatiotemporal mechanism of triggering of receptor-induced Nipah virus fusion and cell entry.
    Authors: Liu, Qian, Stone, Jacquely, Bradel-Tretheway, Birgit, Dabundo, Jeffrey, Benavides Montano, Javier A, Santos-Montanez, Jennifer, Biering, Scott B, Nicola, Anthony, Iorio, Ronald M, Lu, Xiaonan, Aguilar, Hector C
    PLoS Pathog, 2013-11-21;9(11):e1003770.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. EphrinB2 reverse signaling protects against capillary rarefaction and fibrosis after kidney injury.
    Authors: Kida Y, Ieronimakis N, Schrimpf C, Reyes M, Duffield J
    J Am Soc Nephrol, 2013-03-14;24(4):559-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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