Recombinant Human Erythropoietin R Fc Chimera Protein, CF

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Recombinant Human Erythropoietin R Fc Chimera Protein, CF Summary

Product Specifications

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its ability to inhibit Epo-dependent proliferation of TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 1-4 ng/mL in the presence of 0.1 U/mL of recombinant human Epo.
Mouse myeloma cell line, NS0-derived human Erythropoietin R protein
Human Epo R
Accession # P19235
N-terminus C-terminus
Accession #
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
51.4 kDa (monomer)
55-60 kDa, reducing conditions

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Erythropoietin R

Erythropoietin (EPO), a glycoprotein produced primarily by the kidney, is the principal factor that regulates erythropoiesis by stimulating the proliferation and differentiation of erythroid progenitor cells. The biological effects of EPO are mediated by the erythropoietin receptor (EPO R). The genes for human and mouse EPO R have been cloned and characterized. The full-length human EPO R cDNA encodes a type I membrane-spanning protein with 508 amino acid (aa) residues (a 24 aa residue hydrophobic signal sequence, a 226 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 236 aa residue cytoplasmic domain). At the protein sequence level, the human EPO R is approximately 82% identical to the mouse protein. As a result of alternative splicing of the EPO R gene, cDNA clones encoding a truncated form of the EPO R as well as a soluble form of EPO R has been found. The presence of a soluble form of the EPO R has also been detected in human sera. Recombinant soluble EPO R binds EPO with high affinity and is a potent EPO antagonist.

  1. Barber, D.L. and A.D. D’Andrea (1992) Seminars in Hematology 29:293.
  2. Youssoufian, H. et al. (1993) Blood 9:2223.
  3. Lodish, H.F. et al. (1995) Cold Spring Harbor Symposia on Quantitative Biology LX:93.
  4. Baynes, R.D. et al. (1993) Blood 82:2088.
Long Name
Erythropoietin Receptor
Entrez Gene IDs
2057 (Human); 13857 (Mouse)
Alternate Names
EpoR; EPO-R; Erythropoietin R; erythropoietin receptor; MGC138358

Citations for Recombinant Human Erythropoietin R Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Second-generation non-hematopoietic erythropoietin-derived peptide for neuroprotection
    Authors: B Cho, SJ Yoo, SY Kim, CH Lee, YI Lee, SR Lee, C Moon
    Redox Biology, 2021;49(0):102223.
    Species: Human
    Sample Types: Protein
    Applications: Surface Plasmon Resonance
  2. The concentration of brain homogenates with the Amicon Ultra Centrifugal filters
    Authors: J Yang, RK Sumbria
    MethodsX, 2021;8(0):101584.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Capture
  3. Soluble erythropoietin receptor contributes to erythropoietin resistance in end-stage renal disease.
    Authors: Khankin EV, Mutter WP, Tamez H
    PLoS ONE, 2010;5(2):e9246.
    Species: N/A
    Sample Types: N/A
    Applications: Specificity testing
  4. Thrombopoietin contributes to enhanced platelet activation in cigarette smokers.
    Authors: Lupia E, Bosco O, Goffi A
    Atherosclerosis, 2010;210(1):314-9.
    Species: Human
    Sample Types: Plasma
    Applications: Bioassay
  5. Targeted erythropoietin selectively stimulates red blood cell expansion in vivo.
    Authors: DR Burrill, Burrill D, Vernet A, A Vernet, JJ Collins, PA Silver, JC Way, Way J
    Proc Natl Acad Sci U S A, 0;113(19):5245-50.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Enzyme Assay


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