Recombinant Human FCAR/CD89 Protein, CF

  (2 citations)     
Datasheet / CoA / SDS
Product Details
Citations (2)
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  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.01 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to bind human lgA in a functional ELISA with an estimated
    KD <10 nM.
  • Source
    Mouse myeloma cell line, NS0-derived human FCAR/CD89 protein
    Gln22-Asn227, with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    No results obtained: Gln22 predicted
  • Predicted Molecular Mass
    24.3 kDa
  • SDS-PAGE
    40-50 kDa, reducing conditions
Product Datasheets

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3939-FA
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
 
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
 
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
 
Background: FCAR/CD89
FCAR, also called Fc alpha RI or CD89, is a variably glycosylated 50-100 kDa myeloid-specific type I transmembrane (TM) Fc receptor for IgA that is a member of the multichain immune recognition receptor (MIRR) family (1-3). Human FCAR contains a 21 amino acid (aa) signal sequence and extracellular (ECD), TM and cytoplasmic domains of 206, 19 and 41 aa, respectively (4). Arg230 within the TM domain supports interaction with the ITAM-containing signaling subunit, FcR gamma, which contains a TM Asp (5-7). Two ECD C2-type Ig-like domains (EC1 and 2) are oriented at right angles (8). Up to two molecules of FCAR can bind one molecule of serum IgA via EC1 (8). Many splice variants have been reported, but only two have been identified as proteins (9, 10). The a.2 form, which lacks 22 aa just prior to the TM domain, is exclusively expressed in alveolar macrophages. The a.3 form lacks EC2. FCAR binds monomeric, polymeric and secretory IgA, but does not mediate the barrier function of secretory IgA in mucosal epithelium (1-3). Shedding and circulation of polymeric IgA/FCAR immune complexes has been reported (11). Circulating neutrophils, eosinophils, and monocytes express FCAR (12). Tissue expression of FCAR is mainly from neutrophils; FCAR is downregulated as monocytes differentiate to tissue macrophages (12). On neutrophils, a significant amount of FCAR lacks FcR gamma, but can still be endocytosed to early endosomes and recycled to the cell surface (5). Binding of serum IgA to FCAR is transient and anti-inflammatory, inhibiting IgG or IgE-induced degranulation (6). Sustained aggregation of FCAR results in inflammatory responses (6). FcR gamma signaling is required for these and for transport to late endosomes (5-7). Human FCAR shows 55-58% aa identity with rat, horse and cow FCAR. No ortholog occurs in mouse. FCAR structure resembles the KIR/ILT/LIR/MIR family more than other IgA receptors, including pIgR, Fc alpha /μR, asialoglycoprotein receptor (ASGR1) and transferrin receptor (TfR) (1-3).
  • References:
    1. Wines, B. D. and P. M. Hogarth (2006) Tissue Antigens 68:103.
    2. Otten, M. A. and M. van Egmon (2004) Immunol. Lett. 92:23.
    3. Montiero, R. C. and J. G. J. van de Winkel (2003) Annu. Rev. Immunol. 21:177.
    4. Maliszewski, C. R. et al. (1990) J. Exp. Med. 172:1665.
    5. Launay, P. et al. (1999) J. Biol. Chem. 274:7216.
    6. Pasquier, B. et al. (2005) Immunity 22:31.
    7. Shen, L. et al. (2001) Blood 97:205.
    8. Herr, Y. et al. (2003) Nature 423:614.
    9. Patry, C. et al. (1996) J. Immunol. 156:4442.
    10. Togo, S. et al. (2003) FEBS Lett. 535:205.
    11. van der Boog, P. J. M. et al. (2002) J. Immunol. 168:1252.
    12. Hamre, R. et al. (2003) Scand. J. Immunol. 57:506.
  • Long Name:
    IgA Fc Receptor
  • Entrez Gene IDs:
    2204 (Human); 365183 (Rat); 102145896 (Cynomolgus Monkey)
  • Alternate Names:
    CD89 antigen; CD89; CD89IgA Fc receptor; Fc alpha receptor; Fc fragment of IgA, receptor for; FCAR; immunoglobulin alpha Fc receptor
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. An Anti-EGFR IgA That Displays Improved Pharmacokinetics and Myeloid Effector Cell Engagement In Vivo.
    Authors: Lohse S, Meyer S, Meulenbroek L, Jansen J, Nederend M, Kretschmer A, Klausz K, Moginger U, Derer S, Rosner T, Kellner C, Schewe D, Sondermann P, Tiwari S, Kolarich D, Peipp M, Leusen J, Valerius T
    Cancer Res, 2016;76(2):403-17.
    Species: N/A
    Sample Type: Protein
    Application: Surface Plasmon Resonance
  2. Human IgA-binding peptides selected from random peptide libraries: affinity maturation and application in IgA purification.
    Authors: Hatanaka T, Ohzono S, Park M, Sakamoto K, Tsukamoto S, Sugita R, Ishitobi H, Mori T, Ito O, Sorajo K, Sugimura K, Ham S, Ito Y
    J Biol Chem, 2012;287(51):43126-36.
    Species: N/A
    Sample Type: N/A
    Application: Surface Plasmon Resonance

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Primary Antibodies
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WB , Simple Western , Flow , AP , CyTOF-ready MAB050 28  
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ICFlow IC050G  
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ICFlow IC050C  
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Histidine-tagged Protein Purification Resin

IP999 1
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Reconstitution Buffer 1 (PBS)

RB01
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