Recombinant Human FCAR/CD89 Protein, CF

Catalog # Availability Size / Price Qty
3939-FA-050
Product Details
Citations (2)
FAQs
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Recombinant Human FCAR/CD89 Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to bind human lgA in a functional ELISA with an estimated
KD <10 nM.
Source
Mouse myeloma cell line, NS0-derived human FCAR/CD89 protein
Gln22-Asn227, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
No results obtained: Gln22 predicted
Predicted Molecular Mass
24.3 kDa
SDS-PAGE
40-50 kDa, reducing conditions

Product Datasheets

3939-FA

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: FCAR/CD89

FCAR, also called Fc alpha RI or CD89, is a variably glycosylated 50-100 kDa myeloid-specific type I transmembrane (TM) Fc receptor for IgA that is a member of the multichain immune recognition receptor (MIRR) family (1-3). Human FCAR contains a 21 amino acid (aa) signal sequence and extracellular (ECD), TM and cytoplasmic domains of 206, 19 and 41 aa, respectively (4). Arg230 within the TM domain supports interaction with the ITAM-containing signaling subunit, FcR gamma, which contains a TM Asp (5-7). Two ECD C2-type Ig-like domains (EC1 and 2) are oriented at right angles (8). Up to two molecules of FCAR can bind one molecule of serum IgA via EC1 (8). Many splice variants have been reported, but only two have been identified as proteins (9, 10). The a.2 form, which lacks 22 aa just prior to the TM domain, is exclusively expressed in alveolar macrophages. The a.3 form lacks EC2. FCAR binds monomeric, polymeric and secretory IgA, but does not mediate the barrier function of secretory IgA in mucosal epithelium (1-3). Shedding and circulation of polymeric IgA/FCAR immune complexes has been reported (11). Circulating neutrophils, eosinophils, and monocytes express FCAR (12). Tissue expression of FCAR is mainly from neutrophils; FCAR is downregulated as monocytes differentiate to tissue macrophages (12). On neutrophils, a significant amount of FCAR lacks FcR gamma, but can still be endocytosed to early endosomes and recycled to the cell surface (5). Binding of serum IgA to FCAR is transient and anti-inflammatory, inhibiting IgG or IgE-induced degranulation (6). Sustained aggregation of FCAR results in inflammatory responses (6). FcR gamma signaling is required for these and for transport to late endosomes (5-7). Human FCAR shows 55-58% aa identity with rat, horse and cow FCAR. No ortholog occurs in mouse. FCAR structure resembles the KIR/ILT/LIR/MIR family more than other IgA receptors, including pIgR, Fc alpha /μR, asialoglycoprotein receptor (ASGR1) and transferrin receptor (TfR) (1-3).

References
  1. Wines, B. D. and P. M. Hogarth (2006) Tissue Antigens 68:103.
  2. Otten, M. A. and M. van Egmon (2004) Immunol. Lett. 92:23.
  3. Montiero, R. C. and J. G. J. van de Winkel (2003) Annu. Rev. Immunol. 21:177.
  4. Maliszewski, C. R. et al. (1990) J. Exp. Med. 172:1665.
  5. Launay, P. et al. (1999) J. Biol. Chem. 274:7216.
  6. Pasquier, B. et al. (2005) Immunity 22:31.
  7. Shen, L. et al. (2001) Blood 97:205.
  8. Herr, Y. et al. (2003) Nature 423:614.
  9. Patry, C. et al. (1996) J. Immunol. 156:4442.
  10. Togo, S. et al. (2003) FEBS Lett. 535:205.
  11. van der Boog, P. J. M. et al. (2002) J. Immunol. 168:1252.
  12. Hamre, R. et al. (2003) Scand. J. Immunol. 57:506.
Long Name
IgA Fc Receptor
Entrez Gene IDs
2204 (Human); 365183 (Rat); 102145896 (Cynomolgus Monkey)
Alternate Names
CD89 antigen; CD89; CD89IgA Fc receptor; Fc alpha receptor; Fc fragment of IgA, receptor for; FCAR; immunoglobulin alpha Fc receptor

Citations for Recombinant Human FCAR/CD89 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. An Anti-EGFR IgA That Displays Improved Pharmacokinetics and Myeloid Effector Cell Engagement In Vivo.
    Authors: Lohse S, Meyer S, Meulenbroek L, Jansen J, Nederend M, Kretschmer A, Klausz K, Moginger U, Derer S, Rosner T, Kellner C, Schewe D, Sondermann P, Tiwari S, Kolarich D, Peipp M, Leusen J, Valerius T
    Cancer Res, 2016;76(2):403-17.
    Species: N/A
    Sample Types: Protein
    Applications: Surface Plasmon Resonance
  2. Human IgA-binding peptides selected from random peptide libraries: affinity maturation and application in IgA purification.
    Authors: Hatanaka T, Ohzono S, Park M, Sakamoto K, Tsukamoto S, Sugita R, Ishitobi H, Mori T, Ito O, Sorajo K, Sugimura K, Ham S, Ito Y
    J Biol Chem, 2012;287(51):43126-36.
    Species: N/A
    Sample Types: N/A
    Applications: Surface Plasmon Resonance

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