Recombinant Human FCRL4/FcRH4 His-tag Protein, CF

Catalog # Availability Size / Price Qty
2426-FC-050
Recombinant Human FCRL4/FcRH4 His-tag Protein, CF Binding Activity
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Recombinant Human FCRL4/FcRH4 His-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human FCRL4/FcRH4 is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Human IgA binds with an ED50 of 5‑20 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human FCRL4/FcRH4 protein
Ala18-Arg385, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Ala18
Predicted Molecular Mass
42 kDa
SDS-PAGE
53-60 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2426-FC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Binding Activity View Larger

When Recombinant Human FCRL4 (Catalog # 2426-FC) is coatedonto a microplate at 0.5 µg/mL, Biotinylated Human IgA binds with an ED50 of5‑20 μg/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Human FCRL4/FcRH4 was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 53-60 kDa and 45-52 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FCRL4/FcRH4

Fc receptor-like 4 (FCRL4), also known as FcRH4, IFGP2 and IRTA1, is a member of the Ig superfamily transmembrane protein, which is preferentially expressed in B cells and contains immunoreceptor tyrosine-based inhibitory motifs (ITIMs) (1). Mature human FCRL4 consists of a 368 amino acid (aa) extracellular domain (ECD) with four Ig-like C2-type domains, a 21 aa transmembrane segment, and a 107 aa cytoplasmic domain with three ITIM-like motifs. FCRL4 is closely related to FC gamma RIIB (2, 3) and shows high homology to Fc receptors (4). In cellular binding assays, FCRL4 bound to IgA efficiently (5). The FCRL4 gene is localized in the human chromosome 1q21-23 region, a hotspot for translocation events (3). The B cells expressing FCRL4 participate in the autoimmune response in rheumatoid arthritis patients (6).

References
  1. Ehrhardt, G.R. et al. (2003) Proc. Natl. Acad. Sci. U.S.A. 100:13489.
  2. Davis, R.S. et al. (2001) Proc. Natl. Acad. Sci. U. S. A. 98:9772.
  3. Hatzivassiliou, G. et al. (2001) Immunity 14:277.
  4. Amara, K. et al. (2007) J. Autoimmun. 81:34.
  5. Wilson, T.J. et al. (2012) J. Immunol. 188:4741.
  6. Miller, I. et al. (2002) Blood 99:2662.
Long Name
Fc Receptor-like protein 4
Entrez Gene IDs
83417 (Human)
Alternate Names
CD307d antigen; CD307d; Fc receptor homolog 4; Fc receptor-like 4; FcRH4; FCRH4Fc receptor-like protein 4; FCRL4; FcR-like protein 4; hIFGP2; IFGP family protein 2; IFGP2; IGFP2; Immune receptor translocation-associated protein 1; immunoglobulin superfamily Fc receptor, gp42; immunoglobulin superfamily receptor translocation associated 1; IRTA1; IRTA1MGC150523; MGC150522

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