Recombinant Human FCRL5/FcRH5 Protein, CF

    
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  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.01 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to bind human IgG with an estimated KD <30 nM.
  • Source
    Mouse myeloma cell line, NS0-derived human FCRL5/FcRH5 protein
    Gln16-Arg844 with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    No results obtained: Gln16 predicted
  • Predicted Molecular Mass
    91.5 kDa
  • SDS-PAGE
    110-120 kDa, reducing conditions
Product Datasheets

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2078-FC
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
 
Reconstitution Reconstitute at 100 μg/mL in PBS.
 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
 
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
 
Background: FCRL5/FcRH5

Fc Receptor-Like 5 (FCRL5), also known as FcRH5, IRTA2, and CD307, is a 120 kDa protein with sequence homology to classical Fc receptors. The type 1 transmembrane FCRL proteins contain from three to nine immunoglobulin-like domains. They are differentially expressed within the B cell lineage and can either promote or inhibit B cell proliferation and activation (1, 2). According to R&D Systems testing, FCRL5 binds to purified human IgG with high affinity. Mature human FCRL5 consists of a 836 amino acid (aa) extracellular domain (ECD) with nine Ig-like domains, a 21 aa transmembrane segment, and a 105 aa cytoplasmic domain with one immunotyrosine activation motif (ITAM) and two immunotyrosine inhibitory motifs (ITIMs) (1, 3). Mouse FCRL5 contains only five Ig-like domains in its ECD. It shares 49% aa sequence identity with human FCRL5 within common regions. Alternate splicing of human FCRL5 generates isoforms that consist of approximately the first one, six, or eight Ig-like domains (3, 4). FCRL5 expression is restricted to mature B lineage cells in lymphoid tissues and blood (3, 5 - 7). Its ligation inhibits signaling through the B cell antigen receptor (8). Epstein-Barr virus transformation of B cells induces the upregulation of surface FCRL5 by a direct effect of its EBNA2 protein on FCRL5 gene transcription (9). The FCRL5 gene maps to the 1q21 chromosomal locus, a common site of rearrangements in B cell malignancies, and the FCRL5 protein is preferentially expressed in cell lines with 1q21 abnormalities (3). FCRL5 is upregulated on tumor cells in some types of B cell malignancies (6, 10 - 12). In addition, soluble FCRL5 is elevated in the serum of many B cell leukemia patients (11, 13).

  • References:
    1. Davis, R.S. (2007) Annu. Rev. Immunol. 25:525.
    2. Maltais, L.J. et al. (2006) Nat. Immunol. 7:431.
    3. Hatzivassiliou, G. et al. (2001) Immunity 14:277.
    4. SwissProt # Q96RD9
    5. Miller, I. et al. (2002) Blood 99:2662.
    6. Polson, A.G. et al. (2006) Int. Immunol. 18:1363.
    7. Vidal-Laliena, M. et al. (2005) Cell. Immunol. 236:6.
    8. Haga, C.L. et al. (2007) Proc. Natl. Acad. Sci. 104:9770.
    9. Mohan, J. et al. (2006) Blood 107:4433.
    10. Ise, T. et al. (2005) Clin. Cancer Res. 11:87.
    11. Ise, T. et al. (2007) Leukemia 21:169.
    12. Kazemi, T. et al. (2009) Cancer Immunol. Immunother. 58:989.
    13. Ise, T. et al. (2006) Clin. Chem. Lab. Med. 44:594.
  • Long Name:
    Fc Receptor-like 5
  • Entrez Gene IDs:
    83416 (Human); 329693 (Mouse)
  • Alternate Names:
    BXMAS1; BXMAS1MGC119592; CD307; CD307e antigen; CD307e; DKFZp667E2019; DKFZp667F216; Fc receptor homolog 5; Fc receptor-like 5; Fc receptor-like protein 5; FcRH5; FCRH5MGC119593; FCRL5; FcR-like protein 5; FLJ00333; FLJ00397; IFGP5; Immune receptor translocation-associated protein 2; immunoglobulin superfamily receptor translocation associated 2 (IRTA2); IRTA2; IRTA2MGC119590; PRO820
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FAQs

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Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB , Simple Western , Flow , AP , CyTOF-ready MAB050 27  
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His Tag HRP-conjugated Antibody

WB , Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag Biotinylated Antibody

WB BAM050 4  
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His Tag PE-conjugated Antibody

ICFlow IC050P 2  
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His Tag Antibody

WB , Flow , AP MAB050R  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
Description Application Cat# Citations Images  

Histidine-tagged Protein Purification Resin

IP999 1
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