Recombinant Human FCRL5/FcRH5 Protein, CF

Catalog # Availability Size / Price Qty
2078-FC-050
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Recombinant Human FCRL5/FcRH5 Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to bind human IgG with an estimated KD <30 nM.
Source
Mouse myeloma cell line, NS0-derived human FCRL5/FcRH5 protein
Gln16-Arg844 with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
No results obtained: Gln16 predicted
Predicted Molecular Mass
91.5 kDa
SDS-PAGE
110-120 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2078-FC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

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Background: FCRL5/FcRH5

Fc Receptor-Like 5 (FCRL5), also known as FcRH5, IRTA2, and CD307, is a 120 kDa protein with sequence homology to classical Fc receptors. The type 1 transmembrane FCRL proteins contain from three to nine immunoglobulin-like domains. They are differentially expressed within the B cell lineage and can either promote or inhibit B cell proliferation and activation (1, 2). According to R&D Systems testing, FCRL5 binds to purified human IgG with high affinity. Mature human FCRL5 consists of a 836 amino acid (aa) extracellular domain (ECD) with nine Ig-like domains, a 21 aa transmembrane segment, and a 105 aa cytoplasmic domain with one immunotyrosine activation motif (ITAM) and two immunotyrosine inhibitory motifs (ITIMs) (1, 3). Mouse FCRL5 contains only five Ig-like domains in its ECD. It shares 49% aa sequence identity with human FCRL5 within common regions. Alternate splicing of human FCRL5 generates isoforms that consist of approximately the first one, six, or eight Ig-like domains (3, 4). FCRL5 expression is restricted to mature B lineage cells in lymphoid tissues and blood (3, 5 - 7). Its ligation inhibits signaling through the B cell antigen receptor (8). Epstein-Barr virus transformation of B cells induces the upregulation of surface FCRL5 by a direct effect of its EBNA2 protein on FCRL5 gene transcription (9). The FCRL5 gene maps to the 1q21 chromosomal locus, a common site of rearrangements in B cell malignancies, and the FCRL5 protein is preferentially expressed in cell lines with 1q21 abnormalities (3). FCRL5 is upregulated on tumor cells in some types of B cell malignancies (6, 10 - 12). In addition, soluble FCRL5 is elevated in the serum of many B cell leukemia patients (11, 13).

References
  1. Davis, R.S. (2007) Annu. Rev. Immunol. 25:525.
  2. Maltais, L.J. et al. (2006) Nat. Immunol. 7:431.
  3. Hatzivassiliou, G. et al. (2001) Immunity 14:277.
  4. SwissProt # Q96RD9
  5. Miller, I. et al. (2002) Blood 99:2662.
  6. Polson, A.G. et al. (2006) Int. Immunol. 18:1363.
  7. Vidal-Laliena, M. et al. (2005) Cell. Immunol. 236:6.
  8. Haga, C.L. et al. (2007) Proc. Natl. Acad. Sci. 104:9770.
  9. Mohan, J. et al. (2006) Blood 107:4433.
  10. Ise, T. et al. (2005) Clin. Cancer Res. 11:87.
  11. Ise, T. et al. (2007) Leukemia 21:169.
  12. Kazemi, T. et al. (2009) Cancer Immunol. Immunother. 58:989.
  13. Ise, T. et al. (2006) Clin. Chem. Lab. Med. 44:594.
Long Name
Fc Receptor-like 5
Entrez Gene IDs
83416 (Human); 329693 (Mouse)
Alternate Names
BXMAS1; BXMAS1MGC119592; CD307; CD307e antigen; CD307e; DKFZp667E2019; DKFZp667F216; Fc receptor homolog 5; Fc receptor-like 5; Fc receptor-like protein 5; FcRH5; FCRH5MGC119593; FCRL5; FcR-like protein 5; FLJ00333; FLJ00397; IFGP5; Immune receptor translocation-associated protein 2; immunoglobulin superfamily receptor translocation associated 2 (IRTA2); IRTA2; IRTA2MGC119590; PRO820

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