Recombinant Human FGF-9, Animal-Free Protein

R&D Systems | Catalog # Qk039

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Key Product Details

Source

E. coli

Accession Number

P31371.3

Applications

Bioactivity
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Product Specifications

Source

E. coli-derived human FGF-9 protein

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Predicted Molecular Mass

23 kDa

SDS-PAGE

Monomeric FGF-9 protein only

Activity

No significant difference between EC50 of reference and test lots

Mycoplasma

Negative when tested in both ribosomal RNA hybridization and luminescence assays

Scientific Data Images for Recombinant Human FGF-9, Animal-Free Protein

Recombinant Human FGF-9, Animal-Free Protein Bioactivity

FGF-9 activity is determined using the Promega serum response element luciferase reporter assay (*) in transfected HEK293T cells. Cells are treated in triplicate with a serial dilution of FGF-9 for 3 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity. EC50 = 5.7 ng/ml (0.25 nM).*Promega pGL4.33[luc2P/SRE/Hygro] #E1340

Recombinant Human FGF-9, Animal-Free Protein SDS-PAGE

FGF-9 protein migrates as a single band at 23 kDa in non-reducing (NR) conditions and upon reduction (R). No contaminating protein bands are visible. Purified recombinant protein (3 µg) was resolved using 15% w/v SDS-PAGE in reduced (+ beta -mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250.

Formulation, Preparation, and Storage

Qk039
Formulation Lyophilized from HEPES/NaCl
Reconstitution Resuspend in water at >100 µg/ml, prepare single use aliquots, add carrier protein if desired.
Shipping The product is shipped lyophilized at ambient temperture, on ice blocks or dry ice. Shipping at ambient temperture does not affect the bioactivity or stability of the protein. Upon reciept, store immediately at the conditions stated below.
Stability & Storage Store lyophilized protein between -20 and -80 °C until the date of expiry. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FGF-9

Fibroblast growth factor 9 (FGF-9), also known as HBGF9 and GAF, is a member of the FGF family of secreted glycoproteins involved in mammalian skeleton morphogenesis and growth (1). The FGF family is characterized by a core heparin-binding FGF domain of approximately 120 amino acids (aa) that exhibits a beta -trefoil structure (2). Mature mouse FGF-9 shares 99% and 100% aa sequence identity with human and rat FGF-9, respectively. FGF-9, along with FGF-16 and -20, form a FGF subfamily that shares 65-71% aa sequence identity, binds FGFR3(IIIb), and are efficiently secreted despite having an uncleavable, bipartite signal sequence (2-4). In addition to FGFR3(IIIb), FGF-9 binding to the IIIc splice forms of FGFR-1, -2 and -3 have been reported (1,4,6). In the mouse embryo, the location and timing of FGF-9 expression affects development of the skeleton, cerebellum, lungs, heart, vasculature, digestive tract, and testes (2, 6-11). Deletion of mouse FGF-9 is lethal at birth due to lung hypoplasia, and causes rhizomelia, or shortening of the proximal skeleton (2,10,11). Additionally, a mutation in mouse FGF-9 is responsible for Elbow knee synostosis (Eks), which causes joint fusions in the elbow and knee (6). In humans, FGF9 mutations that lower receptor binding can result multiple synostoses syndrome (SYNS) (7). Altered FGF-9 expression or function is reported in human colon, endometrial, and ovarian cancers, correlating with progression, invasiveness, and survival (12-15).

References

  1. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
  2. Itoh, N. and D.M. Ornitz (2008) Dev. Dyn. 237:18.
  3. Miyamoto, M. et al. (1993) Mol. Cell. Biol. 13:4251.
  4. Santos-Ocampo, S. et al. (1996) J. Biol. Chem. 271:1726.
  5. Plotnikov, A.N. et al. (2001) J. Biol. Chem. 276:4322.
  6. Harada, M. et al. (2009) Nat. Genet. 41:289.
  7. Wu, X.L. et al. (2009) Am. J. Hum. Genet. 85:53.
  8. Colvin, J.S. et al. (1999) Dev. Dyn. 216:72.
  9. Lin, Y. et al. (2009) Dev. Biol. 329:44.
  10. Hung, I.H. et al. (2007) Dev. Biol. 307:300.
  11. Colvin, J.S. et al. (2001) Dev. Dyn 128:2095.
  12. Krejci, P. et al. (2009) Hum. Mutat. 30:1245.
  13. Leushacke, M. et al. (2011) PLoS ONE 6:e23381.
  14. Hendrix, N.D. et al. (2006) Cancer Res. 66:1354.
  15. Abdel-Rahman, W.M. et al. (2008) Hum. Mutat. 29:390.

Long Name

Fibroblast Growth Factor 9

Alternate Names

FGF9

Entrez Gene IDs

2254 (Human); 14180 (Mouse)

Gene Symbol

FGF9

UniProt

P31371.3

Additional FGF-9 Products

Product Documents for Recombinant Human FGF-9, Animal-Free Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human FGF-9, Animal-Free Protein

The above product was manufactured, tested and released by R&D System's contract manufacturer, Qkine Ltd, at 1 Murdoch House, Cambridge, UK, CB5 8HW. The product is for research use only and not for the diagnostic or theraputic use.

For research use only

Related Research Areas

FGF Family
FGF Ligands

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