Recombinant Human FGFR2 alpha (IIIb) Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
663-FR-050
R&D Systems Recombinant Proteins and Enzymes
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Citations (2)
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Recombinant Human FGFR2 alpha (IIIb) Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit FGF acidic-dependent proliferation of NR6R‑3T3 mouse fibroblast cells. The ED50 for this effect is 1‑3 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human FGF R2 alpha protein
Human FGF R2 alpha (IIIb)
(Arg22-Glu378)
Accession # CAA39654
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Arg22
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
66 kDa (monomer)
SDS-PAGE
100 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

663-FR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: FGFR2 alpha

Fibroblast growth factors (FGFs) comprise a family of at least eighteen structurally related proteins that are involved in a multitude of physiological and pathological cellular processes, including cell growth, differentiation, angiogenesis, wound healing and tumorgenesis. The biological activities of the FGFs are mediated by a family of type I transmembrane tyrosine kinases which undergo dimerization and autophosphorylation after ligand binding. Four distinct genes encoding closely related FGF receptors, FGF R1 - 4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Multiple forms of FGF R1 - 3 are generated by alternative splicing of the mRNAs. A frequent splicing event involving FGF R1 and 2 results in receptors containing all three Ig domains, referred to as the alpha  isoform, or only IgII and IgIII, referred to as the beta  isoform. Only the alpha  isoform has been identified for FGF R3 and FGF R4. Additional splicing events for FGF R1 - 3, involving the C-terminal half of the IgIII domain encoded by two mutually exclusive alternative exons, generate FGF receptors with alternative IgIII domains (IIIb and IIIc). A IIIa isoform which is a secreted FGF binding protein containing only the N-terminal half of the IgIII domain plus some intron sequences has also been reported for FGF R1. Mutations in FGF R1 - 3 have been found in patients with birth defects involving craniosynostosis. The complex patterns of expression of these receptors as well as the specificity of their interactions with the various FGF ligand family members are under investigation.

References
  1. Galzie, Z. et al. (1997) Biochem. Cell Biol. 75:669.
  2. Burke, D. et al. (1998) Trends Biochem. Sci. 23:59.
Long Name
Fibroblast Growth Factor Receptor 2 alpha
Entrez Gene IDs
2263 (Human); 14183 (Mouse)
Alternate Names
FGF R2a; FGFR2 alpha

Citations for Recombinant Human FGFR2 alpha (IIIb) Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Insulin resistance disrupts epithelial repair and niche-progenitor Fgf signaling during chronic liver injury
    Authors: F Manzano-Nú, MJ Arámbul-An, A Galán Albi, A Leal Tassi, C Acosta Uma, I Borreda Ga, A Herrera, J Forteza Vi, DJ Burks, LA Noon
    PLoS Biol., 2019;17(1):e2006972.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Retinal Proteoglycans Act as Cellular Receptors for Basement Membrane Assembly to Control Astrocyte Migration and Angiogenesis
    Cell Rep, 2016;17(7):1832-1844.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Binding Assay

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