Recombinant Human Fibulin 2 Protein, CF

Catalog # Availability Size / Price Qty
9559-FB-050
Recombinant Human Fibulin 2 Protein Bioactivity
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Recombinant Human Fibulin 2 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of HFL1 human fetal lung fibroblast cells. The ED50 for this effect is 0.15-0.9 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human Fibulin 2 protein
Ala28-Leu1184 (Thr854Ala), with an N-terminal HA tag
Accession #
N-terminal Sequence
Analysis
Tyr (of HA tag)
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
125 kDa
SDS-PAGE
150-175 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9559-FB

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Human Fibulin 2 Protein Bioactivity View Larger

Recombinant Human Fibulin 2 (Catalog # 9559-FB) supports HFL1 human fetal lung fibroblast cell adhesion. The ED50 for this effect is 0.15-0.9 μg/mL

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Background: Fibulin 2

Fibulin 2 is the second largest member of the seven-member fibulin family of extracellular membrane (ECM) glycoproteins. Fibulin-2 consists of 1184 amino acids (aa) with a predicted molecular weight of ~125 kDa. The overall structure is common to fibulins: 3 Anaphylatoxin (AT)-like domains, 11 Epidermal Growth Factor (EGF)‑like domains, and a Fibulin-type carboxy-terminal (FC) domain (1-4). Fibulin 2 is considered a Class I fibulin because the 400-residue N-terminus is divided into the Na and Nb sections, with the Na section containing 150-residues and 12 cysteines while the remaining Nb section is cysteine-free (2, 3). The protein is known to form disulfide-linked homodimers, but it can also be secreted as an oligomer (5). Fibulin 2 is highly conserved across species, with the human protein sharing 82% amino acid identity compared to both mouse and rat. Fibulin 2 is considered a multifunctional binding protein due to its association with numerous ECM components, but its specific interactions have yet to be determined (6). Fibulin 2 is localized at the interface between microfibrils and the elastin core and its known interactions include nidogen-1, perlecan, laminin, aggrecan, endostatins, versican, collagen, and tropoelastin (1, 2, 4, 7). It is down-regulated in numerous forms of cancer including breast, colorectal, lung, esophageal, and squamous cell carcinoma, but over-expression has been shown with solid tumors (1, 3, 4). There is also evidence that suggests that Fibulin 2 may play an indirect role in the neurogenesis of adult neural stem cells via interaction with integrins and TGF-beta 1 (8).

References
  1. Alcendor et al. (2011) Am. J. Pathol. 179:1443.
  2. Timpl et al. (2003) Nat. Rev. Mol. Cell Biol. 4:479.
  3. Kanan et al. (2014) J. Biol. Chem. 289:13419.
  4. Argraves et al. (2003) EMBO reports 4:1127.
  5. Pan et al. (1993) J. Cell Biol. 123:1269.
  6. Fontanil et al. (2014) Oncotarget 5:1253.
  7. El-Hallous et al. (2007) J. Biol. Chem. 282:8935.
  8. Radice et al. (2015) Mol. Cell. Neurosci. 67:75.
Entrez Gene IDs
2199 (Human); 14115 (Mouse); 282583 (Rat)
Alternate Names
FBLN2; FIBL-2; Fibulin 2; Fibulin-2

Citation for Recombinant Human Fibulin 2 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Astrocyte-derived small extracellular vesicles promote synapse formation via fibulin-2-mediated TGF-&beta signaling
    Authors: MR Patel, AM Weaver
    Cell Reports, 2021;34(10):108829.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay

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