Recombinant Human FLRG Protein, CF

Formulations:
Catalog # Availability Size / Price Qty
1288-F3-025/CF
Product Details
Citations (5)
FAQs
Reviews

Recombinant Human FLRG Protein, CF Summary

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to neutralize Activin-mediated erythroid differentiation of K562 human chronic myelogenous leukemia cells. The ED50 for this effect is 3-15 ng/mL in the presence of 7.5 ng/mL recombinant human Activin A.
Source
Mouse myeloma cell line, NS0-derived human Follistatin-related Gene Protein/FLRG protein
Met27-Val263, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Met27
Predicted Molecular Mass
26 kDa
SDS-PAGE
38 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1288-F3/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1288-F3

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Follistatin-related Gene Protein/FLRG

Follistatin-related gene protein (FLRG), also known as follistatin-like 3 (FSTL3) is a glycoprotein belonging to the follistatin-module protein family. Human FLRG cDNA encodes a 263 amino acid (aa) residue protein with a putative 26 aa signal peptide, an N-terminal domain, two cysteine-rich follistatin-like domains (FS) and a C‑terminal acidic domain. Compared to follistatin, FLRG lacks the third FS domain found in follistatin. In addition, FLRG also lacks the heparin-binding domain found within the first amino-terminal FS domain of follistatin. Mouse and human FLRG share approximately 83% aa sequence homology. Like follistatin, FLRG has been shown to bind and inhibit the activities of TGF-beta family ligands including activin, BMP-2, -6, -7 and GDF-8/myostatin. While both FLRG and follistatin are located in a wide and overlapping range of adult and fetal tissue, their sites of peak expression differ: FLRG most highly in heart, lung, kidney, placenta and testis, while follistatin is highest in ovary and pituitary. The expression of FLRG is upregulated by TGF-beta  and activin signaling through Smad proteins. Although FLRG is a secreted protein in many cell types, it has also been localized to the nuclear compartment in HeLa, 293 and CHO cells (1 - 5).

References
  1. Tsuchida, K. et al. (2000) J. Biol. Chem. 275:40778.
  2. Sidis, Y. et al. (2002) Endocrinology 143:1613.
  3. Tortoriello, D.V. et al. (2001) Endocrinology 142:3426.
  4. Hill, J. et al. (2002) J. Biol. Chem. 277:40735.
  5. Bartholin, L. et al. (2001) Oncogene 20:5409.
Entrez Gene IDs
10272 (Human); 83554 (Mouse)
Alternate Names
FLRG; FLRGFSRP; follistatin-like 3 (secreted glycoprotein); Follistatin-like 3; Follistatin-like protein 3; Follistatin-related gene protein; follistatin-related protein 3; FSTL3

Citations for Recombinant Human FLRG Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Structural basis for potency differences between GDF8 and GDF11
    Authors: RG Walker, M Czepnik, EJ Goebel, JC McCoy, A Vujic, M Cho, J Oh, S Aykul, KL Walton, G Schang, DJ Bernard, AP Hinck, CA Harrison, E Martinez-H, AJ Wagers, RT Lee, TB Thompson
    BMC Biol, 2017;15(1):19.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Prediction of gestational diabetes mellitus by maternal factors and biomarkers at 11 to 13 weeks.
    Authors: Nanda S, Savvidou M, Syngelaki A, Akolekar R, Nicolaides KH
    Prenat. Diagn., 2011;31(2):135-41.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  3. Maternal plasma levels of follistatin-related gene protein in the first trimester of pregnancies with Down syndrome.
    Authors: Miron P, Lambert J, Marcil A, Cowans NJ, Stamatopoulou A, Spencer K
    Prenat. Diagn., 2010;30(3):224-8.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  4. Pharmacoproteomics of a metalloproteinase hydroxamate inhibitor in breast cancer cells: dynamics of membrane type 1 matrix metalloproteinase-mediated membrane protein shedding.
    Authors: Butler GS, Dean RA, Tam EM, Overall CM
    Mol. Cell. Biol., 2008;28(15):4896-914.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. BMP4 regulation of human megakaryocytic differentiation is involved in thrombopoietin signaling.
    Authors: Jeanpierre S, Nicolini FE, Kaniewski B, Dumontet C, Rimokh R, Puisieux A, Maguer-Satta V
    Blood, 2008;112(8):3154-63.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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