Recombinant Human FLRT3 Protein, CF

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2795-FL-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human FLRT3 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of Neuro‑2A mouse neuroblastoma cells. Recombinant Human FLRT3 immobilized at 5 μg/mL, 100 μL/well, will mediate >50% Neuro‑2A cell adhesion.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived human FLRT3 protein
Lys29-Pro528, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Lys29
Predicted Molecular Mass
57.3 kDa     
SDS-PAGE
70-90 kDa, under reducing conditions

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2795-FL

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2795-FL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: FLRT3

FLRT3 is one of three FLRT (fibronectin, leucine rich repeat, transmembrane) glycoproteins expressed in distinct areas of the developing brain and other tissues (1, 2). The 85-95 kDa type I transmembrane (TM) human FLRT3 is synthesized as a 649 amino acid (aa) precursor with a 28 aa signal sequence, a 500 aa extracellular domain (ECD), a 21 aa TM segment and a 100 aa cytoplasmic region. The ECD contains 10 N-terminal leucine-rich repeats flanked by cysteine-rich areas, and a juxtamembrane fibronectin type III domain (1). The human FLRT3 ECD shares 96%, 96%, 97%, 97%, 98% and 81% aa sequence identity with mouse, rat, canine, bovine, equine and Xenopus FLRT3 ECD, respectively, and 61% and 48% aa identity to human FLRT2 and FLRT3 ECDs, respectively. The fibronectin domain is responsible for binding to FGF receptors, and is thought to regulate FGF signaling during development (2, 3). The LRR domains are responsible for both the localization in areas of cell contact and homotypic cell-cell association (4). This may be through direct interaction with other FLRT molecules, or alternatively, by regulating internalization of adhesion molecules such as cadherins (4, 5). Developmentally, FLRT3 is located in somitic regions on dermatomyotomal muscle precursors and myotomal cells before their migration to the myotome and syndetome, respectively (2). FLRT3 is also expressed at the midbrain/hindbrain boundary and in the apical ectodermal ridge where it may influence FGF signaling (2). Genetic deletion in mouse embryos results in defective headfold fusion and endoderm migration (6). Postnatally, FLRT3 mRNA is widely expressed (1). It is up-regulated and promotes neurite outgrowth following experimental peripheral nerve injury in rats (7, 8).

 

References
  1. Lacy, S. E. et al. (1999) Genomics 62:417.
  2. Haines, B. P. et al. (2006) Dev. Biol. 297:14.
  3. Bottcher, R. T. et al. (2004) Nat. Cell Biol. 6:38.
  4. Karaulanov, E. E. et al. (2006) EMBO Rep. 7:283.
  5. Ogata, S. et al. (2007) Genes Dev. 21:1817.
  6. Maretto, S. et al. (2008) Dev. Biol. 318:184.
  7. Tsuji, L. et al. (2004) Biochem. Biophys. Res. Commun. 313:1086.
  8. Robinson, M. et al. (2004) Mol. Cell. Neurosci. 27:202.
Long Name
Fibronectin Leucine Rich Transmembrane Protein 3
Entrez Gene IDs
23767 (Human); 71436 (Mouse)
Alternate Names
fibronectin leucine rich transmembrane protein 3; Fibronectin-like domain-containing leucine-rich transmembrane protein 3; FLRT3; HH21; KIAA1469; leucine-rich repeat transmembrane protein FLRT3

Citation for Recombinant Human FLRT3 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Increased expression of fibronectin leucine-rich transmembrane protein 3 in the dorsal root ganglion induces neuropathic pain in rats
    Authors: M Yamada, Y Fujita, Y Hayano, H Hayakawa, K Baba, H Mochizuki, T Yamashita
    J. Neurosci., 2019;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay

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