hh Recombinant Human FSH alpha/beta Protein, CF 5925-FS-010/CF: R&D Systems

Recombinant Human FSH alpha/beta Protein, CF

(2 citations)   
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to induce cAMP accumulation in HEK293 human embryonic kidney cells transfected with human FSH R. The ED50 for this effect is 25-200 pg/mL.
  • Source
    Chinese Hamster Ovary cell line, CHO-derived
    Human FSH alpha  
    Ala25-Ser116
    Accession # P01215
    Human FSH beta
    Cys21-Glu129
    Accession # P01225
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Ala25 (FSH alpha ) & Cys21 (FSH beta )
  • Structure / Form
    Noncovalently-linked heterodimer
  • Predicted Molecular Mass
    10.2 kDa (FSH alpha ) & 12.4 kDa (FSH beta )
  • SDS-PAGE
    22-26 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
5925-FS/CF
 
5925-FS
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images

Recombinant Human FSH alpha / beta (Catalog # 5925-FS/CF) induces cAMP accumulation in the HEK293 human embryonic kidney cell line transfected with human FSH R. The ED50 for this effect is 25-200 pg/mL.

1 µg/lane of Recombinant Human FSH alpha / beta was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing major bands at
20-25 kDa.

Gel Filtration Chromatography

40 µg of Recombinant Human FSH alpha / beta (blue line) was applied onto a Superdex 200 column. The peak eluted at approximately 44 kDa, form overlaid on the molecular weight markers (gray line), corresponds to the dimeric molecular mass.

Background: FSH alpha/beta
Follicle-Stimulating Hormone (FSH) is a 33 kDa heterodimer belonging to the glycoprotein hormone family. It is composed of noncovalently linked glycosylated alpha and beta chains. The alpha subunit is also a component of Luteinizing Hormone (LH), Thyroid-Stimulating Hormone, and Chorionic Gonadotropin. The unique beta subunit confers the protein’s specific biological action and is responsible for the interaction with the FSH Receptor (1-3). The approximately 20 kDa alpha subunit of human FSH shares 73% and 72% aa sequence identity with the mouse and rat orthologs, respectively. The approximately 21 kDa human FSH beta subunit shares 85% and 82% aa sequence identity with the mouse and rat orthologs, respectively. FSH is produced and secreted by the anterior pituitary gland. Its secretion is controlled by Gonadotropin-Releasing Hormone from the hypothalamus; however, FSH secretion can also be stimulated by activins and inhibited by inhibins (4-6). FSH works in concert with LH to regulate female reproduction; FSH stimulates follicular growth and LH induces ovulation (7). FSH can also regulate estrogen formation by inducing the aromatase enzyme that converts androgen to estradiol (7, 8). In addition, it has been suggested to have a role in postmenopausal bone loss and in the neovascularization surrounding malignant tumors (9-11). In males, FSH is critical for sperm production (12). Mutations in the human FSH and FSHR genes are associated with reduced reproductive functioning in females (2, 11). Additionally, increased production of autoantibodies directed against FSH beta has been suggested to contribute to certain types of female infertility (2). Assisted reproductive technology commonly uses recombinant FSH to induce follicle growth (7).
  • References:
    1. Fox, K.M. et al. (2001) Mol. Endocrinol. 15:378.
    2. Haller-Kikkatalo, K. et al. (2012) Clin. Dev. Immunol. 2012:762541.
    3. Jiang, X. et al. (2014) Mol. Cell. Endocrinol. 382:424.
    4. Findlay, J.K. (1993) Biol. Reprod. 48:15.
    5. Marshall, J.C. et al. (1993) Trans. Am. Clin. Climatol. Assoc. 104:31.
    6. Bernard, D.J. and S. Tran (2013) Biol. Reprod. 88:1.
    7. Raju, G.A. et al. (2013) J. Hum. Reprod. Sci. 6:227.
    8. Erickson, G.F. and A.J. Hsueh (1978) Endocrinology 102:1275.
    9. Sun, L. et al. (2006) Cell 125:247.
    10. Radu, A. et al. (2010) N. Engl. J. Med. 363:1621.
    11. Laan, M. et al. (2012) Curr. Opin. Endocrinol. Diabetes Obes. 19:220.
    12. Franchimont, P. et al. (1975) J. Reprod. Fertil. 44:335.
  • Long Name:
    Follicle Stimulating Hormone
  • Entrez Gene IDs:
    2488 (Human); 14308 (Mouse); 25447 (Rat)
  • Alternate Names:
    FSH alpha/beta
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Conserved miR-10 family represses proliferation and induces apoptosis in ovarian granulosa cells
    Authors: T Jiajie, Y Yanzhou, AC Hoi-Hung, C Zi-Jiang, C Wai-Yee
    Sci Rep, 2017;7(0):41304.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  2. In vitro differentiation of human embryonic stem cells into ovarian follicle-like cells
    Authors: D Jung, J Xiong, M Ye, X Qin, L Li, S Cheng, M Luo, J Peng, J Dong, F Tang, W Shen, MM Matzuk, K Kee
    Nat Commun, 2017;8(0):15680.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay

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