Recombinant Human Furin His-tag Protein, CF

R&D Systems | Catalog # 11338-SE

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Key Product Details

  • R&D Systems HEK293-derived Recombinant Human Furin His-tag Protein (11338-SE)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

P09958.2

Structure / Form

Mature

Applications

Enzyme Activity
View all Furin Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Furin protein
Asp108-Ala574, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp108

Predicted Molecular Mass

52 kDa

SDS-PAGE

44-66 kDa, under reducing conditions

Activity

Measured by its ability to cleave the fluorogenic peptide substrate pERTKR-AMC (Catalog # ES013).
The specific activity is >125 pmol/min/μg, as measured under the described conditions.

Scientific Data Images for Recombinant Human Furin His-tag Protein, CF

Recombinant Human Furin His-tag Protein Enzyme Activity.

Recombinant Human Furin (Catalog # 11338-SE) is measured by its ability to cleave the fluorogenic peptide substrate pERTKR-AMC (ES013).

Formulation, Preparation, and Storage

11338-SE
Formulation Supplied as a 0.2 μm filtered solution in Tris, CaCl2, NaCl, Brij-35 and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Furin

Furin is a member of the proprotein convertase (PC) family, which belongs to the subtilisin superfamily of serine protease (1-3). As a cellular protease, Furin cleaves after Arg-Xaa-Lys/Arg-Arg-like motifs typically at the end of the pro region in a variety of proproteins within the secretory pathway including growth factors and receptors, extracellular matrix proteins, and other proteases. Through regulation of proprotein maturation, Furin has an essential role in embryogenesis and homeostasis and is implicated in various pathologies such as cancer (4), neurodegenerative diseases (5) and infectious disease (6). Furin is synthesized as a 794 amino acid type I transmembrane protein precursor with a signal peptide (residues 1-24), a pro region (residues 25-107) that plays a crucial role in the folding, activation and transport of Furin, and a mature chain (residues 108-794) (1-3). The mature chain consists of the subtilisin-like catalytic domain, a P domain, which is essential for enzyme activity and the modulation of pH and calcium requirements, and a cytoplasmic domain, which controls the localization and sorting of Furin in the trans-Golgi network/endosomal system. The purified recombinant human Furin corresponds to a truncated form of the mature enzyme terminated before the transmembrane domain.

References

  1. Van den Ouweland, A.M. et al. (1990) Nucleic Acids Res. 18:664.
  2. Barr, P.J. et al. (1991) DNA Cell Biol. 10:319.
  3. Thomas, G. (2002) Nature Rev. Mol. Cell Biol. 3:753.
  4. He, Z. et al. (2022) Oncogene. 9:1252.
  5. Zhang, Y. et al. (2022) Transl. Neurodegener. 11:39.
  6. Braun, E. and D. Sauter (2019) Clin. Transl. Immunology. 8:e1073.

Alternate Names

FUR, PACE, PCSK3, SPC1

Entrez Gene IDs

5045 (Human); 18550 (Mouse)

Gene Symbol

FURIN

UniProt

P09958.2

Additional Furin Products

Product Documents for Recombinant Human Furin His-tag Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human Furin His-tag Protein, CF

For research use only

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Protocols

View specific protocols for Recombinant Human Furin His-tag Protein, CF (11338-SE):

Materials
  • Assay Buffer: 25 mM Tris, 1 mM CaCl2, 0.5% (w/v) Brij-35, pH 9.0
  • Recombinant Human Furin (rhFurin) (Catalog # 11338-SE)
  • Substrate: p-Glu-Arg-Thr-Lys-Arg-AMC  (Catalog # ES013), 8 mM stock in DMSO
  • Black 96-well Plate
  • Plate Reader with Fluorescence Read Capability
  1. Dilute rhFurin to 4 µg/mL in Assay Buffer.  
  2. Dilute Substrate to 100 µM in Assay Buffer.
  3. Load into a black 96-well plate 50 µL of 4 µg/mL rhFurin, and start the reaction by adding 50 µL of 100 µM Substrate.  Include a Substrate Blank containing 50 µL of Assay Buffer and 50 µL of 100 µM Substrate.
  4. Read plate at excitation and emission wavelengths of 380 nm and 460 nm (top read), respectively, in kinetic mode for 5 minutes.
  5. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)
amount of enzyme (µg)

  

*Adjusted for Substrate Blank
**Derived from calibration standard 7-amino, 4-Methyl Coumarin

Per Well:

  • rhFurin: 0.2 µg
  • Substrate: 50 µM
























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