Recombinant Human G-CSF Protein, CF

(28 citations)   
  • Purity
    >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using NFS‑60 mouse myelogenous leukemia lymphoblast cells. Shirafuji, N.et al. (1989) Exp. Hematol. 17:116. The ED50 for this effect is 10-60 pg/mL.
  • Source
    E. coli-derived Thr31-Pro204, with an N-terminal Met
  • Accession #
  • N-terminal Sequence
    Analysis
    Met
  • Predicted Molecular Mass
    18.8 kDa
  • SDS-PAGE
    18.5 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
214-CS/CF
 
214-CS
Formulation Supplied as a 0.2 μm filtered solution in Acetic Acid.
Formulation Lyophilized from a 0.2 μm filtered solution in Acetic Acid with BSA as a carrier protein.
Reconstitution Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human G-CSF (Catalog # 214-CS/CF) stimulates cell proliferation of the NFS-60 mouse myelogenous leukemia lymphoblast cell line. The ED50 for this effect is 10-60 pg/mL.
1 μg/lane of Recombinant Human G-CSF was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 18.5 kDa.
Background: G-CSF

G-CSF is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma. Other cell types including fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells can also secrete G-CSF after LPS, IL-1 or TNF-alpha activation. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively.

In humans, two distinct cDNA clones for G-CSF, encoding 207 and 204 amino acid precursor proteins, have been isolated. Both proteins have a 30 amino acid signal peptide and have identical amino acid sequences except for a three amino acid insertion (deletion) at the 35th amino acid residue from the N-terminus of the mature protein. Human G-CSF is 73% identical at the amino acid level to murine G-CSF and the two proteins show species cross-reactivity.

In vitro, G-CSF stimulates growth, differentiation and functions of cells from the neutrophil lineage. It also has blast cell growth factor activity and can synergize with IL-3 to shorten the Go period of early hematopoietic progenitors. Consistent with its in vitro functions, G-CSF has been found to play important roles in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis.

  • Long Name:
    Granulocyte Colony Stimulating Factor
  • Entrez Gene IDs:
    1440 (Human); 12985 (Mouse)
  • Alternate Names:
    C17orf33; chromosome 17 open reading frame 33; colony stimulating factor 3 (granulocyte); CSF3; CSF3OS; Filgrastim; GCSF; G-CSF; GCSFlenograstim; granulocyte colony-stimulating factor; Lenograstim; MGC45931; pluripoietin
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

28 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. A Potential Mechanism for ADC-Induced Neutropenia: Role of Neutrophils in Their Own Demise
    Authors: H Zhao, S Gulesseria, MC Malinao, S Kumar-Gane, J Song, MS Chang, MM Williams, Z Zeng, M Mattie, BA Mendelsohn, DR Stover, F Doñate
    Mol. Cancer Ther., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. G-CSF Receptor Blockade Ameliorates Arthritic Pain and Disease
    Authors: MC Lee, JA McCubbin, AD Christense, DP Poole, P Rajasekhar, T Lieu, NW Bunnett, S Garcia-Car, A Erickson, SM Brierley, R Saleh, A Achuthan, AJ Fleetwood, RL Anderson, JA Hamilton, AD Cook
    J. Immunol, 2017;0(0):.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  3. Improved hematopoietic differentiation of human pluripotent stem cells via estrogen receptor signaling pathway
    Cell Biosci, 2016;6(1):50.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  4. Cancer stem cell-secreted macrophage migration inhibitory factor stimulates myeloid derived suppressor cell function and facilitates glioblastoma immune evasion
    Stem Cells, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  5. Transcriptome analysis of Kaposi's sarcoma-associated herpesvirus during de novo primary infection of human B and endothelial cells.
    Authors: Purushothaman P, Thakker S, Verma S
    J Virol, 2015;89(6):3093-111.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  6. Targeted 25-hydroxyvitamin D3 1alpha-hydroxylase adoptive gene therapy ameliorates dss-induced colitis without causing hypercalcemia in mice.
    Authors: Li B, Baylink D, Walter M, Lau K, Meng X, Wang J, Cherkas A, Tang X, Qin X
    Mol Ther, 2015;23(2):339-51.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  7. Preserved in vivo reconstitution ability of PBSCs cryopreserved for a decade at -80 degrees C.
    Authors: Shima T, Iwasaki H, Yamauchi T, Kadowaki M, Kiyosuke M, Mochimaru T, Takenaka K, Miyamoto T, Akashi K, Teshima T
    Bone Marrow Transplant, 2015;50(9):1195-200.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  8. Mouse Resistin (mRetn): cloning, expression and purification in Escherichia coli and the potential regulative effects on murine bone marrow hematopoiesis.
    Authors: Wang F, Gao J, Malisani A, Xi X, Han W, Wan X
    BMC Biotechnol, 2015;15(0):105.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  9. Functional Characterization of D9, a Novel Deazaneplanocin A (DZNep) Analog, in Targeting Acute Myeloid Leukemia (AML).
    Authors: Jiang X, Lim C, Li Z, Lee P, Yatim S, Guan P, Li J, Zhou J, Pan J, Chng W, Chai C, Yu Q
    PLoS ONE, 2015;10(4):e0122983.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  10. Reversible lineage-specific priming of human embryonic stem cells can be exploited to optimize the yield of differentiated cells.
    Authors: Lee J, Graham M, Collins T, Lee J, Hong S, McNicol A, Shapovalova Z, Bhatia M
    Stem Cells, 2015;33(4):1142-52.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  11. Antagonistic determinants controlling replicative and latent states of human cytomegalovirus infection.
    Authors: Umashankar, Mahadeva, Rak, Michael, Bughio, Farah, Zagallo, Patricia, Caviness, Katie, Goodrum, Felicia
    J Virol, 2014;88(11):5987-6002.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  12. Granulocyte-Colony Stimulating Factor related pathways tested on an endometrial ex-vivo model.
    Authors: Rahmati M, Petitbarat M, Dubanchet S, Bensussan A, Chaouat G, Ledee N
    PLoS ONE, 2014;9(9):e102286.
    Species: Human
    Sample Type: Whole Tissue
    Application: Bioassay
  13. A lack of secretory leukocyte protease inhibitor (SLPI) causes defects in granulocytic differentiation.
    Authors: Klimenkova O, Ellerbeck W, Klimiankou M, Unalan M, Kandabarau S, Gigina A, Hussein K, Zeidler C, Welte K, Skokowa J
    Blood, 2014;123(8):1239-49.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  14. Patient-specific naturally gene-reverted induced pluripotent stem cells in recessive dystrophic epidermolysis bullosa.
    Authors: Tolar J, McGrath J, Xia L, Riddle M, Lees C, Eide C, Keene D, Liu L, Osborn M, Lund T, Blazar B, Wagner J
    J Invest Dermatol, 2014;134(5):1246-54.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  15. Cancer-associated adipose tissue promotes breast cancer progression by paracrine oncostatin M and Jak/STAT3 signaling.
    Authors: Lapeire L, Hendrix A, Lambein K, Van Bockstal M, Braems G, Van Den Broecke R, Limame R, Mestdagh P, Vandesompele J, Vanhove C, Maynard D, Lehuede C, Muller C, Valet P, Gespach C, Bracke M, Cocquyt V, Denys H, De Wever O
    Cancer Res, 2014;74(23):6806-19.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  16. G-protein-coupled receptor agonist BV8/prokineticin-2 and STAT3 protein form a feed-forward loop in both normal and malignant myeloid cells.
    Authors: Xin, Hong, Lu, Rongze, Lee, Heehyoun, Zhang, Wang, Zhang, Chunyan, Deng, Jiehui, Liu, Yong, Shen, Shudan, Wagner, Kay-Uwe, Forman, Stephen, Jove, Richard, Yu, Hua
    J Biol Chem, 2013;288(19):13842-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  17. The canonical Wnt pathway shapes niches supportive of hematopoietic stem/progenitor cells.
    Authors: Ichii, Michiko, Frank, Mark Bar, Iozzo, Renato V, Kincade, Paul W
    Blood, 2012;119(7):1683-92.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  18. A novel serum-free monolayer culture for orderly hematopoietic differentiation of human pluripotent cells via mesodermal progenitors.
    Authors: Niwa A, Heike T, Umeda K
    PLoS ONE, 2011;6(7):e22261.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  19. Hematopoietic differentiation of induced pluripotent stem cells from patients with mucopolysaccharidosis type I (Hurler syndrome).
    Authors: Tolar J, Park I, Xia L, Lees C, Peacock B, Webber B, McElmurry R, Eide C, Orchard P, Kyba M, Osborn M, Lund T, Wagner J, Daley G, Blazar B
    Blood, 2011;117(3):839-47.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  20. ETV6-RUNX1 promotes survival of early B lineage progenitor cells via a dysregulated erythropoietin receptor.
    Authors: Torrano V, Procter J, Cardus P
    Blood, 2011;118(18):4910-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  21. Does granulocyte colony-stimulating factor ameliorate the proinflammatory response in human meningococcal septic shock?
    Authors: Rojahn A, Brusletto B, Ovstebo R, Haug KB, Kierulf P, Brandtzaeg P
    Crit. Care Med., 2008;36(9):2583-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  22. Isolation and characterization of CD146+ multipotent mesenchymal stromal cells.
    Authors: Sorrentino A, Ferracin M, Castelli G, Biffoni M, Tomaselli G, Baiocchi M, Fatica A, Negrini M, Peschle C, Valtieri M
    Exp. Hematol., 2008;36(8):1035-46.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  23. Unique structural determinants for Stat3 recruitment and activation by the granulocyte colony-stimulating factor receptor at phosphotyrosine ligands 704 and 744.
    Authors: Shao H, Xu X, Jing N, Tweardy DJ
    J. Immunol., 2006;176(5):2933-41.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  24. Proteinase-activated receptor2 agonists upregulate granulocyte colony-stimulating factor, IL-8, and VCAM-1 expression in human bronchial fibroblasts.
    Authors: Ramachandran R, Morice AH, Compton SJ
    Am. J. Respir. Cell Mol. Biol., 2006;35(1):133-41.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  25. Proinflammatory mediators elicit secretion of the intracellular B-lymphocyte stimulator pool (BLyS) that is stored in activated neutrophils: implications for inflammatory diseases.
    Authors: Scapini P, Carletto A, Nardelli B, Calzetti F, Roschke V, Merigo F, Tamassia N, Pieropan S, Biasi D, Sbarbati A, Sozzani S, Bambara L, Cassatella MA
    Blood, 2005;105(2):830-7.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  26. IL-22 increases the innate immunity of tissues.
    Authors: Wolk K, Kunz S, Witte E, Friedrich M, Asadullah K, Sabat R
    Immunity, 2004;21(2):241-54.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  27. Enhanced circulating half-life and hematopoietic properties of a human granulocyte colony-stimulating factor/immunoglobulin fusion protein.
    Authors: Cox GN, Smith DJ, Carlson SJ, Bendele AM, Chlipala EA, Doherty DH
    Exp. Hematol., 2004;32(5):441-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  28. Proliferating dendritic cell progenitors in human blood.
    Authors: Romani N, Gruner S, Brang D, Kampgen E, Lenz A, Trockenbacher B, Konwalinka G, Fritsch P, Steinman R, Schuler G
    J Exp Med, 1994;180(1):83-93.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
Expand to show all 28 Citations
Animal-Free Recombinant Proteins
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Recombinant Human G-CSF, Animal-Free Protein

BA AFL214
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