Recombinant Human G-CSF Protein

Formulations:
Catalog # Availability Size / Price Qty
214-CS-005
214-CS-025
Recombinant Human G-CSF Protein Data
2 Images
Product Details
Citations (33)
FAQs
Supplemental Products
Reviews (1)

Recombinant Human G-CSF Protein Summary

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using NFS‑60 mouse myelogenous leukemia lymphoblast cells. Shirafuji, N.et al. (1989) Exp. Hematol. 17:116. The ED50 for this effect is 10-60 pg/mL.
Source
E. coli-derived human G-CSF protein
Thr31-Pro204, with an N-terminal Met
Accession #
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
18.8 kDa
SDS-PAGE
17-19 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

214-CS

Formulation Lyophilized from a 0.2 μm filtered solution in Acetic Acid with BSA as a carrier protein.
Reconstitution Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

214-CS/CF

Formulation Supplied as a 0.2 μm filtered solution in Acetic Acid.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Do not freeze.Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, 2 to 8 °C as supplied.

Data Images

Bioactivity View Larger

Recombinant Human G-CSF (Catalog # 214-CS) stimulates cell proliferation of the NFS-60 mouse myelogenous leukemia lymphoblast cell line. The ED50for this effect is 10-60 pg/mL.

SDS-PAGE View Larger

1 μg/lane of Recombinant Human G-CSF was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 18.5 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: G-CSF

G-CSF is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma. Other cell types including fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells can also secrete G-CSF after LPS, IL-1 or TNF-alpha activation. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively.

In humans, two distinct cDNA clones for G-CSF, encoding 207 and 204 amino acid precursor proteins, have been isolated. Both proteins have a 30 amino acid signal peptide and have identical amino acid sequences except for a three amino acid insertion (deletion) at the 35th amino acid residue from the N-terminus of the mature protein. Human G-CSF is 73% identical at the amino acid level to murine G-CSF and the two proteins show species cross-reactivity.

In vitro, G-CSF stimulates growth, differentiation and functions of cells from the neutrophil lineage. It also has blast cell growth factor activity and can synergize with IL-3 to shorten the Go period of early hematopoietic progenitors. Consistent with its in vitro functions, G-CSF has been found to play important roles in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis.

Long Name
Granulocyte Colony Stimulating Factor
Entrez Gene IDs
1440 (Human); 12985 (Mouse)
Alternate Names
C17orf33; chromosome 17 open reading frame 33; colony stimulating factor 3 (granulocyte); CSF3; CSF3OS; Filgrastim; GCSF; G-CSF; GCSFlenograstim; granulocyte colony-stimulating factor; Lenograstim; MGC45931; Pluripoietin

Citations for Recombinant Human G-CSF Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

33 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Targeting macrophage checkpoint inhibitor SIRPa for anticancer therapy
    Authors: J Liu, S Xavy, S Mihardja, S Chen, K Sompalli, D Feng, TS Choi, B Agoram, R Majeti, IL Weissman, JP Volkmer
    JCI Insight, 2020;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Therapeutic discovery for marrow failure with MDS predisposition using pluripotent stem cells
    Authors: M Ruiz-Gutie, ÖV Bölükba??, G Alexe, AG Kotini, K Ballotti, CE Joyce, DW Russell, K Stegmaier, K Myers, CD Novina, EP Papapetrou, A Shimamura
    JCI Insight, 2019;5(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Differentiation
  3. CRISPR/Cas9 mediated ELANE knockout enables neutrophilic maturation of primary hematopoietic stem and progenitor cells and induced pluripotent stem cells of severe congenital neutropenia patients
    Authors: M Nasri, M Ritter, P Mir, B Dannenmann, N Aghaallaei, D Amend, V Makaryan, Y Xu, B Fletcher, R Bernhard, I Steiert, K Hahnel, J Berger, I Koch, B Sailer, K Hipp, C Zeidler, M Klimiankou, B Bajoghli, DC Dale, K Welte, J Skokowa
    Haematologica, 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Molecular transitions in early progenitors during human cord blood hematopoiesis
    Authors: S Zheng, E Papalexi, A Butler, W Stephenson, R Satija
    Mol. Syst. Biol., 2018;14(3):e8041.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. G-CSF Receptor Blockade Ameliorates Arthritic Pain and Disease
    Authors: MC Lee, JA McCubbin, AD Christense, DP Poole, P Rajasekhar, T Lieu, NW Bunnett, S Garcia-Car, A Erickson, SM Brierley, R Saleh, A Achuthan, AJ Fleetwood, RL Anderson, JA Hamilton, AD Cook
    J. Immunol, 2017;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  6. A Potential Mechanism for ADC-Induced Neutropenia: Role of Neutrophils in Their Own Demise
    Authors: H Zhao, S Gulesseria, MC Malinao, S Kumar-Gane, J Song, MS Chang, MM Williams, Z Zeng, M Mattie, BA Mendelsohn, DR Stover, F Doñate
    Mol. Cancer Ther., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Cancer stem cell-secreted macrophage migration inhibitory factor stimulates myeloid derived suppressor cell function and facilitates glioblastoma immune evasion
    Stem Cells, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Differentiation of induced pluripotent stem cell-derived neutrophil granulocytes from common marmoset monkey (Callithrix jacchus)
    Authors: Thomas Müller
    Transfusion, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Improved hematopoietic differentiation of human pluripotent stem cells via estrogen receptor signaling pathway
    Cell Biosci, 2016;6(1):50.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Functional Characterization of D9, a Novel Deazaneplanocin A (DZNep) Analog, in Targeting Acute Myeloid Leukemia (AML).
    Authors: Jiang X, Lim C, Li Z, Lee P, Yatim S, Guan P, Li J, Zhou J, Pan J, Chng W, Chai C, Yu Q
    PLoS ONE, 2015;10(4):e0122983.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Transcriptome analysis of Kaposi's sarcoma-associated herpesvirus during de novo primary infection of human B and endothelial cells.
    Authors: Purushothaman P, Thakker S, Verma S
    J Virol, 2015;89(6):3093-111.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Reversible lineage-specific priming of human embryonic stem cells can be exploited to optimize the yield of differentiated cells.
    Authors: Lee J, Graham M, Collins T, Lee J, Hong S, McNicol A, Shapovalova Z, Bhatia M
    Stem Cells, 2015;33(4):1142-52.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Mouse Resistin (mRetn): cloning, expression and purification in Escherichia coli and the potential regulative effects on murine bone marrow hematopoiesis.
    Authors: Wang F, Gao J, Malisani A, Xi X, Han W, Wan X
    BMC Biotechnol, 2015;15(0):105.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Targeted 25-hydroxyvitamin D3 1alpha-hydroxylase adoptive gene therapy ameliorates dss-induced colitis without causing hypercalcemia in mice.
    Authors: Li B, Baylink D, Walter M, Lau K, Meng X, Wang J, Cherkas A, Tang X, Qin X
    Mol Ther, 2015;23(2):339-51.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Preserved in vivo reconstitution ability of PBSCs cryopreserved for a decade at -80 degrees C.
    Authors: Shima T, Iwasaki H, Yamauchi T, Kadowaki M, Kiyosuke M, Mochimaru T, Takenaka K, Miyamoto T, Akashi K, Teshima T
    Bone Marrow Transplant, 2015;50(9):1195-200.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. A lack of secretory leukocyte protease inhibitor (SLPI) causes defects in granulocytic differentiation.
    Authors: Klimenkova O, Ellerbeck W, Klimiankou M, Unalan M, Kandabarau S, Gigina A, Hussein K, Zeidler C, Welte K, Skokowa J
    Blood, 2014;123(8):1239-49.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Granulocyte-Colony Stimulating Factor related pathways tested on an endometrial ex-vivo model.
    Authors: Rahmati M, Petitbarat M, Dubanchet S, Bensussan A, Chaouat G, Ledee N
    PLoS ONE, 2014;9(9):e102286.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Bioassay
  18. Cancer-associated adipose tissue promotes breast cancer progression by paracrine oncostatin M and Jak/STAT3 signaling.
    Authors: Lapeire L, Hendrix A, Lambein K, Van Bockstal M, Braems G, Van Den Broecke R, Limame R, Mestdagh P, Vandesompele J, Vanhove C, Maynard D, Lehuede C, Muller C, Valet P, Gespach C, Bracke M, Cocquyt V, Denys H, De Wever O
    Cancer Res, 2014;74(23):6806-19.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Patient-specific naturally gene-reverted induced pluripotent stem cells in recessive dystrophic epidermolysis bullosa.
    Authors: Tolar J, McGrath J, Xia L, Riddle M, Lees C, Eide C, Keene D, Liu L, Osborn M, Lund T, Blazar B, Wagner J
    J Invest Dermatol, 2014;134(5):1246-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Antagonistic determinants controlling replicative and latent states of human cytomegalovirus infection.
    Authors: Umashankar, Mahadeva, Rak, Michael, Bughio, Farah, Zagallo, Patricia, Caviness, Katie, Goodrum, Felicia
    J Virol, 2014;88(11):5987-6002.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  21. G-protein-coupled receptor agonist BV8/prokineticin-2 and STAT3 protein form a feed-forward loop in both normal and malignant myeloid cells.
    Authors: Xin, Hong, Lu, Rongze, Lee, Heehyoun, Zhang, Wang, Zhang, Chunyan, Deng, Jiehui, Liu, Yong, Shen, Shudan, Wagner, Kay-Uwe, Forman, Stephen, Jove, Richard, Yu, Hua
    J Biol Chem, 2013;288(19):13842-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  22. The canonical Wnt pathway shapes niches supportive of hematopoietic stem/progenitor cells.
    Authors: Ichii, Michiko, Frank, Mark Bar, Iozzo, Renato V, Kincade, Paul W
    Blood, 2012;119(7):1683-92.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  23. A novel serum-free monolayer culture for orderly hematopoietic differentiation of human pluripotent cells via mesodermal progenitors.
    Authors: Niwa A, Heike T, Umeda K
    PLoS ONE, 2011;6(7):e22261.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  24. ETV6-RUNX1 promotes survival of early B lineage progenitor cells via a dysregulated erythropoietin receptor.
    Authors: Torrano V, Procter J, Cardus P
    Blood, 2011;118(18):4910-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Hematopoietic differentiation of induced pluripotent stem cells from patients with mucopolysaccharidosis type I (Hurler syndrome).
    Authors: Tolar J, Park I, Xia L, Lees C, Peacock B, Webber B, McElmurry R, Eide C, Orchard P, Kyba M, Osborn M, Lund T, Wagner J, Daley G, Blazar B
    Blood, 2011;117(3):839-47.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  26. Isolation and characterization of CD146+ multipotent mesenchymal stromal cells.
    Authors: Sorrentino A, Ferracin M, Castelli G, Biffoni M, Tomaselli G, Baiocchi M, Fatica A, Negrini M, Peschle C, Valtieri M
    Exp. Hematol., 2008;36(8):1035-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  27. Does granulocyte colony-stimulating factor ameliorate the proinflammatory response in human meningococcal septic shock?
    Authors: Rojahn A, Brusletto B, Ovstebo R, Haug KB, Kierulf P, Brandtzaeg P
    Crit. Care Med., 2008;36(9):2583-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  28. Proteinase-activated receptor2 agonists upregulate granulocyte colony-stimulating factor, IL-8, and VCAM-1 expression in human bronchial fibroblasts.
    Authors: Ramachandran R, Morice AH, Compton SJ
    Am. J. Respir. Cell Mol. Biol., 2006;35(1):133-41.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  29. Unique structural determinants for Stat3 recruitment and activation by the granulocyte colony-stimulating factor receptor at phosphotyrosine ligands 704 and 744.
    Authors: Shao H, Xu X, Jing N, Tweardy DJ
    J. Immunol., 2006;176(5):2933-41.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  30. Proinflammatory mediators elicit secretion of the intracellular B-lymphocyte stimulator pool (BLyS) that is stored in activated neutrophils: implications for inflammatory diseases.
    Authors: Scapini P, Carletto A, Nardelli B, Calzetti F, Roschke V, Merigo F, Tamassia N, Pieropan S, Biasi D, Sbarbati A, Sozzani S, Bambara L, Cassatella MA
    Blood, 2005;105(2):830-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  31. Enhanced circulating half-life and hematopoietic properties of a human granulocyte colony-stimulating factor/immunoglobulin fusion protein.
    Authors: Cox GN, Smith DJ, Carlson SJ, Bendele AM, Chlipala EA, Doherty DH
    Exp. Hematol., 2004;32(5):441-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  32. IL-22 increases the innate immunity of tissues.
    Authors: Wolk K, Kunz S, Witte E, Friedrich M, Asadullah K, Sabat R
    Immunity, 2004;21(2):241-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  33. Proliferating dendritic cell progenitors in human blood.
    Authors: Romani N, Gruner S, Brang D, Kampgen E, Lenz A, Trockenbacher B, Konwalinka G, Fritsch P, Steinman R, Schuler G
    J Exp Med, 1994;180(1):83-93.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Animal-Free Recombinant Proteins

Reviews for Recombinant Human G-CSF Protein

Average Rating: 5 (Based on 1 Review)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Recombinant Human G-CSF Protein?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Recombinant Human G-CSF Protein
By Anonymous on 11/06/2018
Application: Immunoassay Standard

The recombinant G-CSF was used as a calibrator in an ELISA with sensitivity at 18pg/ml. The R&D Systems G-CSF antibody MAB214 was used as the capture in this assay along with BAF214 as the detection.