Recombinant Human G6b Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
5499-G6-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human G6b Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When 1 μg/mL of rhG6b/Fc Chimera is captured by a goat anti-human IgG Fc Chimera coated plate, the concentration of biotinylated heparin that produces 50% of the optimal binding response is found to be approximately 10-40 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human G6b protein
Human G6b
(Asn18-Gln142)
Accession # O95866
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Asn18
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
40.0 kDa (monomer)
SDS-PAGE
45-55 kDa under reducing conditions

Product Datasheets

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5499-G6

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5499-G6

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: G6b

G6b is a type I transmembrane glycoprotein in the immunoglobulin superfamily that is encoded within the human class III major histocompatibility complex (1-3). It is expressed mainly on platelets, and has also been detected on megakaryocytes and the human cell line K562 (2-5). Crosslinking of G6b inhibits agonist-stimulated platelet aggregation and activation (5). The 241 amino acid (aa) human G6b isotype B (G6b-B) contains a 17 aa signal sequence, a 125 aa extracellular domain with one V-type Ig-like domain and one N-linked glycosylation site, a 21 aa transmembrane sequence and a 78 aa cytoplasmic sequence with two ITIM inhibitory motifs and a SH3 binding site (1, 2). Phosphorylation at Y211 within the first ITIM is required for interaction with SHP adaptor/tyrosine phosphatase proteins (2, 3). Of several described isoforms, all are identical within aa 22-136, which includes most of the extracellular domain. One contains an alternate signal sequence, while at least two lack a transmembrane sequence and are secreted (2). At least two others, including G6b-A, express alternate C-termini that lack the ITIM motifs. Of the known isoforms, G6b-A and G6b-B predominate in platelets. It is possible that G6b-A may be paired with and control inhibition by G6b-B (4). Heparin interacts with the G6b extracellular domain with high-affinity and may contribute to G6b activity or adhesion to extracellular matrix (6).

References
  1. Ribas, G. et al. (1999) J. Immunol. 163:278.
  2. DeVet, E. C. J. M. et al. (2001) J. Biol. Chem. 276:42070.
  3. Senis, Y. A. et al. (2007) Mol. Cell. Proteomics 6:548.
  4. Macaulay, I. C. et al. (2007) Blood 109:3260.
  5. Newland, S. A. et al. (2007) Blood 109:4806.
  6. DeVet, E. C. J. M. et al. (2005) FEBS Lett. 579:2355.
Long Name
Glycoprotein VI [Platelet]
Entrez Gene IDs
80739 (Human); 106722 (Mouse); 406865 (Rat)
Alternate Names
C6orf25; chromosome 6 open reading frame 25; G6b; immunoglobulin receptor; MGC142279; MGC142281; NG31; protein G6b

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