Galectin-1, gene name LGALS1 (lectin, galactoside-binding, soluble 1), is a 135 amino acid (aa), 14 kDa, pleiotropic, non-glycosylated, monomeric or homodimeric carbohydrate-binding protein of the prototype galectin family (1-3). Galectins lack a classical signal peptide and can be localized to the cytosolic compartments, or secreted via non-classical pathways (1). Secreted Galectin-1 has immunosuppressive and anti-inflammatory properties and suppresses acute and chronic inflammation and autoimmunity. It contributes to negative selection of developing T cells, immunosuppression by regulatory T cells, resolution of the inflammatory response, and inhibition of immune cell migration, inflammatory cytokine production, and mast cell degranulation (1, 2, 4-6). Galectin-1 preferentially binds laminin, fibronectin, 90K/Mac-2BP, CD45, CD43, CD7, CD2, CD3, integrins alpha 4 beta 1, alpha 5 beta 1 and alpha 4 beta 7, and ganglioside GM1 (2, 3). It is produced in a variety of tissues by cells that include endothelial cells, connective tissue fibroblasts, thymic stromal cells, tumor cells, muscle cells, platelets, regulatory T cells, and activated tissue macrophages, B cells, T cells and dendritic cells (2, 3, 6-11). Most of this expression is cytosolic. Human Galectin-1 shares 88% aa sequence identity with mouse, equine and ovine, 90% with rat, and 87% with bovine and porcine Galectin-1. Endothelial cell surface expression, including tumor endothelial cells, is greatly increased by cell activation (9). Galectin-1 is highly expressed at the maternal-fetal interface and contributes to fetal immune privilege (5, 12). Its immunosuppressive properties appear to also allow tumor cells to evade immune detection (4, 5). It selectively controls T cell survival by inducing apoptosis of activated Th1 and Th17 cells, which express Galectin-1-binding glycans, while promoting Th2 cell survival where glycans are sialylated and less recognized (4, 13). It also induces apoptosis of immature thymocytes (3, 6). Galectin-1 secreted from bone marrow stromal cells aids B lymphocyte development by contributing to pre-B cell integrin adhesion and receptor signaling (3). The dimer form of Galectin-1 also induces neutrophil down‑regulation by inducing cell surface exposure of phosphatidylserine that marks the cell for phagocytosis (14). Galectin-1 can also modulate cell-cell and cell-matrix interactions, and can promote either cell attachment or detachment depending on the cell type and developmental stage (1, 2).
Recombinant Human Galectin-1 Protein
R&D Systems | Catalog # 1152-GA
Key Product Details
- R&D Systems E. coli-derived Recombinant Human Galectin-1 Protein (1152-GA)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
Accession Number
Applications
Product Specifications
Source
Ala2-Asp135
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
The ED50 for this effect is 0.5‑3 µg/mL.
Reviewed Applications
Read 2 reviews rated 5 using 1152-GA in the following applications:
Scientific Data Images for Recombinant Human Galectin-1 Protein
Recombinant Human Galectin-1 Protein SDS-PAGE.
2 μg/lane of Recombinant Human Galectin-1 Protein (Catalog # 1152-GA) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 11-14 kDa.Formulation, Preparation, and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 1152-GA
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS and Betamercaptoethanol with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 1152-GA/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS and Betamercaptoethanol. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Calculators
Background: Galectin-1
References
- Di Lella, S. et al. (2011) Biochemistry 50:7842.
- Rabinovich, G.A. and J.M. Ilarregui (2009) Immunol. Rev. 230:144.
- Rabinovich, G.A. and M. Vidal. (2011) Curr. Opin. Hematol. 18:443.
- Cedeno-Laurent, F. and C.J. Dimitroff (2012) Clin. Immunol. 142:107.
- Than, N.G. et al. (2012) Trends Endocrinol. Metab. 23:23.
- Rubinstein, N. et al. (2004) Tissue Antigens 64:1.
- Courad, P.O. et al. (1989) J. Biol. Chem. 264:1310.
- Hirabayashi, J. et al. (1989) Biochim. Biophys. Acta 1008:85.
- Thijssen, V.L. et al. (2008) Am. J. Pathol. 172:545.
- Nio-Kobayashi, J. et al. (2009) J. Histochem. Cytochem. 57:41.
- Dias-Baruffi, M. et al. (2010) Glycobiology 20:507.
- Than, N.G. et al. (2008) Proc. Natl. Acad. Sci. USA 105:15819.
- Toscano, M.A. et al. (2007) Nat. Immunol. 8:825.
- Stowell, S.R. et al. (2009) J. Biol. Chem. 284:4989.
Alternate Names
Gene Symbol
UniProt
Additional Galectin-1 Products
Product Documents for Recombinant Human Galectin-1 Protein
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human Galectin-1 Protein
For research use only
Related Research Areas
Citations for Recombinant Human Galectin-1 Protein
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Customer Images
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Application: growing cellsVerified Customer | Posted 03/13/2019
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Sample Tested:Verified Customer | Posted 10/26/2015The protein is well preseved and showed the exact size as expected.
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