Recombinant Human GCNT2 His-tag Protein, CF
R&D Systems | Catalog # 10847-GT
Glucosaminyl (N-acetyl) Transferase 2
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Key Product Details
- R&D Systems CHO-derived Recombinant Human GCNT2 His-tag Protein (10847-GT)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
CHO
Accession Number
Applications
Enzyme Activity
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Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived human Glucosaminyl (N-acetyl) Transferase 2/GCNT2 protein
Asn26-Phe400, with a C-terminal 6-His
Asn26-Phe400, with a C-terminal 6-His
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Asn26
Predicted Molecular Mass
43.8 kDa
SDS-PAGE
53-67 kDa, under reducing conditions.
Activity
Measured by its ability to transfer GlcNAc from UDP-GlcNAc to Cy5-labeled Extended G2.
0.25 μg of Recombinant Human GCNT2 will convert >50% of its substrate to product, as measured under the described conditions.
0.25 μg of Recombinant Human GCNT2 will convert >50% of its substrate to product, as measured under the described conditions.
Scientific Data Images for Recombinant Human GCNT2 His-tag Protein, CF
Recombinant Human GCNT2 His-tag Protein/GCNT2 Enzyme Activity Diagram.
GCNT2 (Catalog # 10847-GT) recognizes polylactosamine structure and can tolerate core-6 fucose and its Cy5 derivatives.
Recombinant Human GCNT2 His-tag Protein, CF SDS-PAGE
1 μg/lane of Recombinant Human GCNT2 His-tag (Catalog # 10847-GT) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing bands at 53-63 kDa.
Recombinant Human GCNT2 His-tag Protein, CF Fluorescent Gel Mobility Shift
Lane 1 contained substrate glycan extended G2 (GL303). In the presence of rhGCNT2, the glycan was modified, and a mobility shift was observed.Formulation, Preparation, and Storage
10847-GT
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Glucosaminyl (N-acetyl) Transferase 2/GCNT2
References
- Marti, F.A. et al. (1995) Glycobiology 5:417.
- Bierhuizen, M.F.A. et al. (1993) Genes Dev. 7:468.
- Inaba, N. et al. (2003) blood 101:2870.
- Sweeney, Jenna Geddes et al. (2018) Nature communications 9:3368.
- Haijun, Zhang et al. (2011) Cancer Res. 71:4846.
- Wu, Z.L. et al (2020) Glycobiology 30:970.
Long Name
Glucosaminyl (N-Acetyl) Transferase 2, I-Branching Enzyme (I Blood Group)
Alternate Names
CCAT, CTRCT13, GCNT2C, NACGT1, NAGCT1, ULG3
Gene Symbol
GCNT2
UniProt
Additional Glucosaminyl (N-acetyl) Transferase 2/GCNT2 Products
Product Documents for Recombinant Human GCNT2 His-tag Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human GCNT2 His-tag Protein, CF
For research use only
Related Research Areas
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Protocols
View specific protocols for Recombinant Human GCNT2 His-tag Protein, CF (10847-GT):
Materials
- Assay Buffer: 50 mM MES, 1 mM DTT, pH 6.5
- Recombinant Human GCNT2 (rhGCNT2) (Catalog # 10847-GT)
- Cy5-labeled Extended G2 (Catalog # GL303)
- Recombinant Human B4GalT1 (rhB4GALT1) (Catalog # 3609-GT)
- UDP-Gal (Sigma, Catalog # U4500), 10 mM stock in Deionized Water
- UDP-GlcNAc (Sigma, Catalog # U4375), 50 mM stock in 50% ethanol/50% Deionized Water
15% SDS-PAGE Gel - Reducing SDS-PAGE gel loading buffer
- Fluorescent imager
- Dilute rhGCNT2 to 25 µg/mL in Assay Buffer.
- Prepare a Master Mix containing 0.2 µM Cy5-labeled Extended G2, 1 mM UDP-GlcNAc, 1 mM UDP-Gal and 50 µg/mL rhB4GALT1 in Assay Buffer.
- Prepare reaction(s) by combining 10 µL of 25 µg/mL rhGCNT2 and 10 µL of Master Mix. Prepare a negative control by combining 10 µL of Assay Buffer and 10 µL of Master Mix.
- Incubate the reaction(s) and control at 37 °C for 90 minutes.
- Add 7 µL of reducing SDS-PAGE gel loading buffer to each reaction and control. Mix.
- Load 13.5 µL of each reaction and control per lane on a 15% SDS-PAGE gel. Run dye front down 80% of the length of the gel (minimum).
- Visualize the gel with a fluorescent imager.
- Calculate percent conversion of the substrate to product.
Per Reaction:
- rhGCNT2: 0.25 µg
- Cy5-labeled Extended G2: 2 pmol
- UDP-Gal: 0.5 mM
- UDP-GlcNAc: 0.5 mM
- rhB4GALT1: 0.5 µg
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