Recombinant Human GFR alpha-like His-tag Protein, CF

Catalog # Availability Size / Price Qty
9647-GR-050
Recombinant Human GFR alpha-like His-tag Protein, CF Bioactivity
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Recombinant Human GFR alpha-like His-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human GFR alpha ‑like is coated at 0.1 μg/mL, 100 μL/well, it binds to Recombinant GDF-15 (Catalog # 9279-GD) with an ED50 of 0.15-0.9 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived human GFR alpha-like protein
Ser19-Glu351, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Ser19
Predicted Molecular Mass
39 kDa
SDS-PAGE
37-54 kDa, reducing conditions

Product Datasheets

9647-GR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Human GFR alpha-like His-tag Protein, CF Bioactivity View Larger

When Recombinant Human GFR alpha ‑like (Catalog # 9647‑GR) is immobilizedat 0.1 μg/mL, 100 μL/well, it binds to Recombinant Human GDF-15 (Catalog # 9279‑GD) with an ED50 of 0.15‑0.9 ng/mL.

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Background: GFR alpha-like

GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins (1). Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain. Over the extracellular domain, human GFRAL shares 72% and 71% identity with mouse and rat GFRAL respectively.  GFRAL is expressed on both fetal and adult hindbrain neurons of the CNS (3), and would appear to function as an anti-apoptotic molecule during neuronal stress. GFRAL is a functional receptor for GDF-15, facilitating weight-loss functions of the protein through c-Ret downstream signaling. GFRAL and GDF-15 signaling is implicated in diet-based obesity and insulin resistance (2-4).

References
  1. Li, Z. et al. (2005) J. Neurochem. 95:361.
  2. Mullican, S. et al. (2017) Nat. Med 23:1150.
  3. Yang, L. et al. (2017) Nat. Med 23:1158.
  4. Emmerson, P. et al. (2017) Nat. Med 23:1215.
Long Name
Glial Cell line-derived Neurotrophic Factor Receptor alpha-like
Entrez Gene IDs
389400 (Human); 404194 (Mouse); 501023 (Rat)
Alternate Names
GFR alpha like; GFR alpha-like; GFRAL; GRAL

FAQs

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Primary Antibodies

His Tag Antibody

WB,Simple Western,Flow,AP,CyTOF-ready

Protein Purification Kits

Reconstitution Buffers

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