>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When Recombinant Human GFR alpha ‑like is coated
at 0.1 μg/mL, 100 μL/well, it binds to Recombinant GDF-15
with an ED50 of 0.15-0.9 ng/mL.
Human embryonic kidney cell, HEK293-derived Ser19-Glu351, with a C-terminal 6-His tag
When Recombinant Human GFR alpha ‑like (Catalog # 9647‑GR) is immobilizedat 0.1 μg/mL, 100 μL/well, it binds to Recombinant Human GDF-15 (Catalog # 9279‑GD) with an ED50 of 0.15‑0.9 ng/mL.
Background: GFR alpha-like
alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family
of proteins (1). Mature human GFR alpha-like is a 376 amino acid (aa)
type I transmembrane protein. It contains a 333 aa extracellular domain, a
20 aa transmembrane domain and a 23 aa cytoplasmic domain. Over the
extracellular domain, human GFRAL shares 72% and 71% identity with mouse and rat GFRAL
respectively. GFRAL is expressed on both
fetal and adult hindbrain neurons of the CNS (3), and would appear to function
as an anti-apoptotic molecule during neuronal stress. GFRAL is a functional receptor for GDF-15,
facilitating weight-loss functions of the protein through c-Ret downstream
and GDF-15 signaling is implicated in diet-based obesity and insulin resistance
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