Recombinant Human GFR alpha-like His-tag Protein, CF

Catalog # Availability Size / Price Qty
9647-GR-050
Recombinant Human GFR alpha-like His-tag Protein, CF Bioactivity
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Recombinant Human GFR alpha-like His-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human GFR alpha ‑like is coated at 0.1 μg/mL, 100 μL/well, it binds to Recombinant GDF-15 (Catalog # 9279-GD) with an ED50 of 0.15-0.9 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived human GFR alpha-like protein
Ser19-Glu351, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Ser19
Predicted Molecular Mass
39 kDa
SDS-PAGE
37-54 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9647-GR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Bioactivity View Larger

When Recombinant Human GFR alpha ‑like (Catalog # 9647‑GR) is immobilizedat 0.1 μg/mL, 100 μL/well, it binds to Recombinant Human GDF-15 (Catalog # 9279‑GD) with an ED50 of 0.15‑0.9 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GFR alpha-like

GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins (1). Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain. Over the extracellular domain, human GFRAL shares 72% and 71% identity with mouse and rat GFRAL respectively.  GFRAL is expressed on both fetal and adult hindbrain neurons of the CNS (3), and would appear to function as an anti-apoptotic molecule during neuronal stress. GFRAL is a functional receptor for GDF-15, facilitating weight-loss functions of the protein through c-Ret downstream signaling. GFRAL and GDF-15 signaling is implicated in diet-based obesity and insulin resistance (2-4).

References
  1. Li, Z. et al. (2005) J. Neurochem. 95:361.
  2. Mullican, S. et al. (2017) Nat. Med 23:1150.
  3. Yang, L. et al. (2017) Nat. Med 23:1158.
  4. Emmerson, P. et al. (2017) Nat. Med 23:1215.
Long Name
Glial Cell line-derived Neurotrophic Factor Receptor alpha-like
Entrez Gene IDs
389400 (Human); 404194 (Mouse); 501023 (Rat)
Alternate Names
GFR alpha like; GFR alpha-like; GFRAL; GRAL

FAQs

No product specific FAQs exist for this product, however you may

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Primary Antibodies

His Tag Antibody

WB,Simple Western,Flow,AP,CyTOF-ready

Protein Purification Kits

Reconstitution Buffers

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