Recombinant Human GFR alpha-like His-tag Protein, CF

  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When Recombinant Human GFR alpha ‑like is coated at 0.1 μg/mL, 100 μL/well, it binds to Recombinant GDF-15 (Catalog # 9279-GD) with an ED50 of 0.15-0.9 ng/mL.
  • Source
    Human embryonic kidney cell, HEK293-derived Ser19-Glu351, with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Ser19
  • Predicted Molecular Mass
    39 kDa
  • SDS-PAGE
    37-54 kDa, reducing conditions
9647-GR
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
When Recombinant Human GFR alpha ‑like (Catalog # 9647‑GR) is immobilizedat 0.1 μg/mL, 100 μL/well, it binds to Recombinant Human GDF-15 (Catalog # 9279‑GD) with an ED50 of 0.15‑0.9 ng/mL.
Background: GFR alpha-like
GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins (1). Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain. Over the extracellular domain, human GFRAL shares 72% and 71% identity with mouse and rat GFRAL respectively.  GFRAL is expressed on both fetal and adult hindbrain neurons of the CNS (3), and would appear to function as an anti-apoptotic molecule during neuronal stress. GFRAL is a functional receptor for GDF-15, facilitating weight-loss functions of the protein through c-Ret downstream signaling. GFRAL and GDF-15 signaling is implicated in diet-based obesity and insulin resistance (2-4).
  • References:
    1. Li, Z. et al. (2005) J. Neurochem. 95:361.
    2. Mullican, S. et al. (2017) Nat. Med 23:1150.
    3. Yang, L. et al. (2017) Nat. Med 23:1158.
    4. Emmerson, P. et al. (2017) Nat. Med 23:1215.
  • Long Name:
    Glial Cell line-derived Neurotrophic Factor Receptor alpha-like
  • Entrez Gene IDs:
    389400 (Human); 404194 (Mouse); 501023 (Rat)
  • Alternate Names:
    GFR alpha like; GFR alpha-like; GFRAL; GRAL
Related Research Areas
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB, Simple Western, Flow, AP, CyTOF-ready MAB050 26  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag HRP-conjugated Antibody

WB, Simple Western MAB050H 3  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag APC-conjugated Antibody

ICFlow IC050A  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Biotinylated Antibody

WB BAM050 4  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag PE-conjugated Antibody

ICFlow IC050P 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Antibody

WB, Flow, AP MAB050R  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag PerCP-conjugated Antibody

ICFlow IC050C  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Alexa Fluor® 405-conjugated Antibody

ICFlow IC050V
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Alexa Fluor® 750-conjugated Antibody

ICFlow IC050S
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Alexa Fluor® 594-conjugated Antibody

ICFlow IC0501T
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Alexa Fluor® 594-conjugated Antibody

ICFlow IC050T
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC0501N
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Alexa Fluor® 750-conjugated Antibody

ICFlow IC0501S
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Alexa Fluor® 405-conjugated Antibody

ICFlow IC0501V
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Alexa Fluor® 647-conjugated Antibody

ICFlow IC0501R
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Alexa Fluor® 350-conjugated Antibody

ICFlow IC050U
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Alexa Fluor® 350-conjugated Antibody

ICFlow IC0501U
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC0501G
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Alexa Fluor® 647-conjugated Antibody

ICFlow IC050R
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Protein Purification Kits
Description Application Cat# Citations Images  

Histidine-tagged Protein Purification Resin

IP999 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Recombinant Human GFR alpha-like His-tag Protein, CF?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

    Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Feedback

Customer Information