Recombinant Human GIF Protein, CF

Catalog # Availability Size / Price Qty
8387-TC-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human GIF Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Bioassay data are not available.
Source
Human embryonic kidney cell, HEK293-derived human GIF protein
Ser19-Tyr417 with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Ser19
Predicted Molecular Mass
44 kDa
SDS-PAGE
44-60 kDa, reducing conditions

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8387-TC

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8387-TC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in sterile water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Reconstitution Calculator

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Background: GIF

Gastric Intrinsic Factor (GIF) also known as Intrinsic Factor (IF) is a 45-55 kDa glycoprotein that is secreted by parietal cells of the gastric mucosa. GIF is a member of the eukaryotic cobalamin (Cbl or Vitamin B12) family of transport proteins that mediate the uptake of cobalamin by intestinal epithelial cells lining the ileum (1). GIF comprises two domains, an alpha and a beta -domain containing approximatively 270 and 110 residues respectively (2). Cobalamin binds to GIF at the interface of the alpha  and beta  domains (2). The Cbl-GIF complex can then interact with its specific receptor, Cubilin, through the amino-terminal portion of GIF (3). This complex associates with Amnionless (AMN) which directs subcellular localization and endocytosis of Cubilin and its ligands (4). Homozygous nonsense and missense mutations in the GIF gene have been linked to hereditary juvenile megaloblastic anemia. These patients show nutritional cobalamin insufficiency due to its intestinal malabsorption (5). Mature human GIF shares 82% and 79% amino acid sequence identity with mouse GIF and rat GIF respectively.

References
  1. Hewitt, J.E. et al. (1991) Genomics 10:432.
  2. Mathews, F.S. et al. (2007) Proc. Natl. Acad. Sci. USA 104:17311.
  3. Tang, L-H. et al. (1992) J. Biol. Chem. 267:22982.
  4. Fyfe, J.C. et al. (2004) Blood 103:1573.
  5. Tanner, S.M. et al. (2005) Proc. Natl. Acad. Sci. USA 102:4130.
Long Name
Gastric Intrinsic Factor
Entrez Gene IDs
2694 (Human); 14603 (Mouse); 29319 (Rat)
Alternate Names
gastric intrinsic factor (vitamin B synthesis); GIF; IF; IFIntrinsic factor; IFMH; IFMHgastric intrinsic factor; INF; TCN3

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