Recombinant Human GPR111 Protein, CF

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R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human GPR111 Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by the ability of the immobilized protein to support the adhesion of U‑87 MG human glioblastoma/astrocytoma cells. The ED50 for this effect is 1-5 μg/mL.
Chinese Hamster Ovary cell line, CHO-derived human GPR111 protein
Cys19-Ser375, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Predicted Molecular Mass
40.9 kDa
55-66 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: GPR111

GPR111, also known as PGR20, is an approximately 70 kDa member of the LN-7TM family of adhesion G protein-coupled receptors. Like other LN-7TM family proteins, it contains an extended N-terminal extracellular region followed by a series of seven transmembrane (TM) segments and a short C-terminal cytoplasmic tail (1, 2). The N-terminal domain contains a mucin-like stalk and a juxtamembrane region that resembles the GPS motif found in other LN-7TM proteins. The GPS motif is a component of the conserved GAIN domain which mediates the autoproteolysis and shedding of a wide range of proteins including LN-7TM proteins (3). The GPS-like motif in GPR111, however, is divergent from the consensus motif and does not appear to be cleavable (3, 4). Alternative splicing of human GPR111 generates a long isoform with a 92 aa substitution for the N-terminal 24 residues as well as a 19 aa substitution in the final TM segment and cytoplasmic tail. This recombinant protein product corresponds to the N-terminal extracellular domain of the shorter isoform (aa 19-375). Within this region, human GPR111 shares approximately 68% aa sequence identity with mouse and rat GPR111. GPR111 is expressed in squamous epithelia of the skin, esophagus, tongue epidermis, and stomach (4).

  1. Fredriksson, R. et al. (2002) FEBS Lett. 531:407.
  2. Bjarnadottir, T.K. et al. (2004) Genomics 84:23.
  3. Arac, D. et al. (2012) EMBO J. 31:1364.
  4. Promel, S. et al. (2012) Dev. Dyn. 241:1591.
Long Name
G-Protein-Coupled Receptor 111
Entrez Gene IDs
222611 (Human); 435529 (Mouse); 301269 (Rat)
Alternate Names
G protein-coupled receptor 111; GPR111; PGR20


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