Recombinant Human GPR111 Protein, CF

Catalog # Availability Size / Price Qty
6494-GP-050
R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Recombinant Human GPR111 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of U‑87 MG human glioblastoma/astrocytoma cells. The ED50 for this effect is 1-5 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human GPR111 protein
Cys19-Ser375, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Cys19
Predicted Molecular Mass
40.9 kDa
SDS-PAGE
55-66 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6494-GP

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: GPR111

GPR111, also known as PGR20, is an approximately 70 kDa member of the LN-7TM family of adhesion G protein-coupled receptors. Like other LN-7TM family proteins, it contains an extended N-terminal extracellular region followed by a series of seven transmembrane (TM) segments and a short C-terminal cytoplasmic tail (1, 2). The N-terminal domain contains a mucin-like stalk and a juxtamembrane region that resembles the GPS motif found in other LN-7TM proteins. The GPS motif is a component of the conserved GAIN domain which mediates the autoproteolysis and shedding of a wide range of proteins including LN-7TM proteins (3). The GPS-like motif in GPR111, however, is divergent from the consensus motif and does not appear to be cleavable (3, 4). Alternative splicing of human GPR111 generates a long isoform with a 92 aa substitution for the N-terminal 24 residues as well as a 19 aa substitution in the final TM segment and cytoplasmic tail. This recombinant protein product corresponds to the N-terminal extracellular domain of the shorter isoform (aa 19-375). Within this region, human GPR111 shares approximately 68% aa sequence identity with mouse and rat GPR111. GPR111 is expressed in squamous epithelia of the skin, esophagus, tongue epidermis, and stomach (4).

References
  1. Fredriksson, R. et al. (2002) FEBS Lett. 531:407.
  2. Bjarnadottir, T.K. et al. (2004) Genomics 84:23.
  3. Arac, D. et al. (2012) EMBO J. 31:1364.
  4. Promel, S. et al. (2012) Dev. Dyn. 241:1591.
Long Name
G-Protein-Coupled Receptor 111
Entrez Gene IDs
222611 (Human); 435529 (Mouse); 301269 (Rat)
Alternate Names
G protein-coupled receptor 111; GPR111; PGR20

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reconstitution Buffers

Reviews for Recombinant Human GPR111 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human GPR111 Protein, CF and earn rewards!

Have you used Recombinant Human GPR111 Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review