Recombinant Human GPR158 His-tag Protein, CF

Catalog # Availability Size / Price Qty
10286-GP-050
Recombinant Human GPR158 His-tag Protein, CF Binding Activity
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Recombinant Human GPR158 His-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human GPR158 is immobilized at 1 µg/mL (100 µL/well), it binds to Recombinant Human Neuropilin-1 (Catalog # 3870-N1) with an ED50 of 0.8-4.8 μg/mL
Source
Human embryonic kidney cell, HEK293-derived human GPR158 protein
Ala24-Gln411, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Ala24 & Ser25
Predicted Molecular Mass
45 kDa
SDS-PAGE
50-70 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10286-GP

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 1 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Binding Activity View Larger

When Recombinant Human GPR158 (Catalog # 10286-GP) is coated at 1 μg/mL, 100 μL/well, Recombinant Human Neuropilin-1 (Catalog # 3870-N1) binds with an ED50 of 0.8‑4 μg/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Human GPR158 His-tag Protein (Catalog # 10286-GP) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 50-70 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GPR158

G-protein coupled receptor 158 (GPR158) is a receptor belonging to the Class C GPCR family. It lacks the extracellular Venus flytrap module characteristic of the known members of that family and instead contains two other elements that are not typical of the class: a calcium-binding EGF-like domain and a leucine repeat region (1, 2). The mature extracellular domain of human GPR158 contains 393 amino acids (aa) and shares 89% identity with both mouse and rat GPR158. GPR158 is expressed at the highest level in the brain, but also in a variety of other tissues including retina, spleen, liver and lung (3). GPR158 was originally identified in functional screens linked with biological stress and has been implicated in the osteocalcin effect on cognitive processes in the brain (4, 5), and glaucoma and cancer in the periphery (4, 6).

References
  1. Jingami, H. et al. (2003) Curr. Opin. Neurobiol. 13:271.
  2. Bjarnadóttir, T.K et al. (2005) Gene. 362:70.
  3. Orlandi, C. et al. (2012) J. Cell Biol. 197:711.
  4. Itakura, T. et al. (2019) J. Ocul. Pharmacol. Ther. 35:203.
  5. Khrimian, L. et al. (2017) J. Exp. Med. 214:2859.
  6. Fenner, A. (2015) Nat. Rev. Urol. 12:182.
Entrez Gene IDs
57512 (Human); 241263 (Mouse); 291352 (Rat)
Alternate Names
G protein-coupled receptor 158; GPR158; KIAA1136; KIAA1136FLJ37801; probable G-protein coupled receptor 158; RP11-59G22.1

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