Recombinant Human Neuropilin-1 Protein, CF

His-tag
Catalog # Availability Size / Price Qty
3870-N1-025

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Recombinant Human Neuropilin-1 Protein, CF Summary

Why choose R&D Systems Neuropilin-1Protein?

  • Guaranteed Bioactivity and High Purity: Bioactivity tested by functional ELISA and purity determined by SDS-PAGE to be greater than 95%.
  • Lot-to-Lot Consistency: Stringent QC testing performed on each lot to ensure consistent activity and purity.
  • Bulk Quantities Available: Bulk up and save with large mass quantities to meet your research needs. Supply agreements available, partner with us. Please contact us.
  • Most Respected, Most Cited Brand in Proteins: With over 35 years of providing the best recombinant proteins to the scientific community, R&D Systems continues to lead the industry in quality, activity, and purity.

Find the Fc chimera Neuropilin-1 protein here Neuropilin-1 Fc Chimera

Other Neuropilin-1 species available Neuropilin-1 Proteins

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Neuropilin-1 can bind recombinant human VEGF165 with an apparent Kd <1 nM.
Source
Mouse myeloma cell line, NS0-derived human Neuropilin-1 protein
Phe22-Lys644, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Phe22
Predicted Molecular Mass
70.6 kDa
SDS-PAGE
85-95 kDa, reducing conditions

Product Datasheets

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3870-N1

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3870-N1

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Neuropilin-1

Neuropilin-1 protein (Nrp1, previously neuropilin; also CD304) is a 130 - 140 kDa type I transmembrane (TM) glycoprotein that regulates axon guidance and angiogenesis (1 - 4). The full-length 923 amino acid (aa) human NRP1 contains a 623 aa extracellular domain (ECD) that shows 92 - 95% aa identity with mouse, rat, bovine and canine NRP1 (3, 4). The NRP1 ECD contains two N-terminal CUB domains (termed a1a2), two domains with homology to coagulation factors V and VIII (b1b2) and a MAM (meprin) domain (c). C-terminally divergent splice variants with 704, 644, 609, and 551 aa lack the MAM and TM domains and are demonstrated or presumed to be soluble antagonists (1, 5 - 7). A 906 aa form lacks a TM segment, but secretion has not been found (8). The sema domains of Class III secreted semaphorins such as Sema3A bind NRP1 a1a2 (9). Heparin, the heparin-binding forms of VEGF (VEGF165, VEGF-B and VEGF-E), PlGF (PlGF2), and the C-terminus of Sema3 bind the b1b2 region (9, 10). NRP1 and NRP2 share 48% aa identity within the ECD and can form homo- and hetero-oligomers via interaction of their MAM domains (1). Neuropilins show partially overlapping expression in neuronal and endothelial cells during development (1, 2). Both neuropilins act as co-receptors with plexins, mainly plexin A3 and A4, to bind class III semaphorins that mediate axon repulsion (11). However, only NRP1 binds Sema3A, and only NRP2 binds Sema3F (1). Both are co-receptors with VEGF R2 (also called KDR or Flk-1) for VEGF165 binding (1). Sema3A signaling can be blocked by VEGF165, which has higher affinity for NRP1 (12). NRP1 is preferentially expressed in arteries during development or those undergoing remodeling (1, 2). NRP1 is also expressed on dendritic cells and mediates DC-induced T cell proliferation (13).

Neuropilin-1 has been shown to bind to the S1 domain of the SARS-CoV-2 Spike protein via its b1 domain (14,15). Blocking this interaction reduced viral entry and infectivity in vitro providing a new potential therapeutic target to treat COVID-19.

References
  1. Bielenberg, D.R. et al. (2006) Exp. Cell Res. 312:584. 
  2. Gu, C. et al. (2003) Dev. Cell 5:45. 
  3. He, Z. and M. Tessier-Lavigne (1997) Cell 90:739.
  4. Soker, S. et al. (1998) Cell 92:735. 
  5. Gagnon, M.L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:2573. 
  6. Cackowski, F.C. et al. (2004) Genomics 84:82. 
  7. Rossignol, M. et al. (2000) Genomics 70:211.
  8. Tao, Q. et al. (2003) Angiogenesis 6:39.
  9. Gu, C. et al. (2002) J. Biol. Chem. 277:18069.
  10. Mamluk, R. et al. (2002) J. Biol. Chem. 277:24818.
  11. Yaron, A. et al. (2005) Neuron 45:513.
  12. Narazaki, M. and G. Tosato (2006) Blood 107:3892.
  13. Tordjman, R. et al. (2002) Nat. Immunol. 3477.
  14. Daly, J.L. et al. (2020) Science. Oct 20:eabd3072. doi: 10.1126/science.abd3072.
  15. Cantuti-Castelvetri L. et al. (2020) Science. Oct 20:eabd2985. doi: 10.1126/science.abd2985.
Entrez Gene IDs
8829 (Human); 18186 (Mouse); 246331 (Rat); 100286859 (Porcine)
Alternate Names
BDCA4; BDCA-4; CD304 antigen; CD304; DKFZp686A03134; DKFZp781F1414; neuropilin 1; Neuropilin1; Neuropilin-1; NP1; NRP; NRP1; transmembrane receptor; Vascular endothelial cell growth factor 165 receptor; VEGF165R

Citations for Recombinant Human Neuropilin-1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. A Novel Blood-Brain Barrier-Penetrating and Vascular-Targeting Chimeric Peptide Inhibits Glioma Angiogenesis
    Authors: Lu, L;Wang, L;Zhao, L;Liao, J;Zhao, C;Xu, X;Wang, F;Zhang, X;
    International journal of molecular sciences
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance (SPR)
  2. Resilience to autosomal dominant Alzheimer's disease in a Reelin-COLBOS heterozygous man
    Authors: Lopera, F;Marino, C;Chandrahas, AS;O'Hare, M;Villalba-Moreno, ND;Aguillon, D;Baena, A;Sanchez, JS;Vila-Castelar, C;Ramirez Gomez, L;Chmielewska, N;Oliveira, GM;Littau, JL;Hartmann, K;Park, K;Krasemann, S;Glatzel, M;Schoemaker, D;Gonzalez-Buendia, L;Delgado-Tirado, S;Arevalo-Alquichire, S;Saez-Torres, KL;Amarnani, D;Kim, LA;Mazzarino, RC;Gordon, H;Bocanegra, Y;Villegas, A;Gai, X;Bootwalla, M;Ji, J;Shen, L;Kosik, KS;Su, Y;Chen, Y;Schultz, A;Sperling, RA;Johnson, K;Reiman, EM;Sepulveda-Falla, D;Arboleda-Velasquez, JF;Quiroz, YT;
    Nature medicine
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bio-Layer Interferometry (BLI), Bioassay
  3. Targeting Neuropilin-1 with Nanobodies Reduces Colorectal Carcinoma Development
    Authors: Y De Vlaemin, S Bonelli, RM Awad, M Dewilde, S Rizzolio, Q Lecocq, E Bolli, AR Santos, D Laoui, S Schoonoogh, L Tamagnone, C Goyvaerts, M Mazzone, K Breckpot, JA Van Ginder
    Cancers, 2020-11-30;12(12):.
    Species: Vicugna pacos (alpaca)
    Sample Types: In Vivo
    Applications: In Vivo
  4. A Dual Receptor Targeting- and BBB Penetrating- Peptide Functionalized Polyethyleneimine Nanocomplex for Secretory Endostatin Gene Delivery to Malignant Glioma
    Authors: L Lu, H Chen, L Wang, L Zhao, Y Cheng, A Wang, F Wang, X Zhang
    Int J Nanomedicine, 2020-11-12;15(0):8875-8892.
    Species: Human
    Sample Types: Protein
    Applications: SPR
  5. Soluble St2 Induces Cardiac Fibroblast Activation and Collagen Synthesis via Neuropilin-1
    Authors: L Matilla, V Arrieta, E Jover, A Garcia-Peñ, E Martinez-M, R Sadaba, V Alvarez, A Navarro, A Fernandez-, A Gainza, E Santamaria, J Fernandez-, P Rossignol, F Zannad, N Lopez-Andr
    Cells, 2020-07-10;9(7):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  6. An Unbiased Screen for Human Cytomegalovirus Identifies Neuropilin-2 as a Central Viral Receptor.
    Authors: Martinez-Martin N, Marcandalli J, Huang C, Arthur C, Perotti M, Foglierini M, Ho H, Dosey A, Shriver S, Payandeh J, Leitner A, Lanzavecchia A, Perez L, Ciferri C
    Cell, 2018-07-26;174(5):1158-1171.e19.
    Species: Virus
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance (SPR
  7. B7-H4 Modulates Regulatory CD4+ T Cell Induction and Function via Ligation of a Semaphorin 3a/Plexin A4/Neuropilin-1 Complex
    Authors: JR Podojil, MY Chiang, I Ifergan, R Copeland, LN Liu, S Maloveste, S Langermann, D Liebenson, R Balabanov, H Chi, L Chen, DAA Vignali, SD Miller
    J. Immunol., 2018-06-13;0(0):.
    Species: Human, Mouse
    Sample Types: Recombinant Protein, Whole Cells
    Applications: Bioassay, Direct ELISA
  8. Neuropilin-1 mediates Neutrophil Elastase uptake and cross-presentation in breast cancer cells
    Authors: C Kerros, SC Tripathi, D Zha, JM Mehrens, A Sergeeva, AV Philips, N Qiao, HL Peters, H Katayama, P Sukhumalch, KE Ruisaard, AA Perakis, LS St John, S Lu, EA Mittendorf, K Clise-Dwye, AC Herrmann, G Alatrash, C Toniatti, SM Hanash, Q Ma, JJ Molldrem
    J. Biol. Chem., 2017-05-03;0(0):.
    Applications: Bioassay
  9. Synergistic Binding of Vascular Endothelial Growth Factor-A and Its Receptors to Heparin Selectively Modulates Complex Affinity.
    Authors: Teran M, Nugent M
    J Biol Chem, 2015-05-15;290(26):16451-62.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  10. The propeptides of VEGF-D determine heparin binding, receptor heterodimerization, and effects on tumor biology.
    Authors: Harris, Nicole C, Davydova, Natalia, Roufail, Sally, Paquet-Fifield, Sophie, Paavonen, Karri, Karnezis, Tara, Zhang, You-Fang, Sato, Teruhiko, Rothacker, Julie, Nice, Edouard, Stacker, Steven A, Achen, Marc G
    J Biol Chem, 2013-02-12;288(12):8176-86.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Precipitation
  11. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012-07-26;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Exogenous recombinant dimeric neuropilin-1 is sufficient to drive angiogenesis.
    Authors: Uniewicz KA, Cross MJ, Fernig DG
    J. Biol. Chem., 2010-10-18;286(1):12-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Oligo-guanosine nucleotide induces neuropilin-1 internalization in endothelial cells and inhibits angiogenesis.
    Authors: Narazaki M, Segarra M, Hou X, Tanaka T, Li X, Tosato G
    Blood, 2010-07-06;116(16):3099-107.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Binding Assay, Surface Plasmon Resonance
  14. The original Pathologische Anatomie Leiden-Endothelium monoclonal antibody recognizes a vascular endothelial growth factor binding site within neuropilin-1.
    Authors: Jaalouk DE, Ozawa MG, Sun J, Lahdenranta J, Schlingemann RO, Pasqualini R, Arap W
    Cancer Res., 2007-10-15;67(20):9623-9.
    Species: Mouse
    Sample Types: Protein
    Applications: Bioassay

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Recombinant Human Neuropilin-1 Protein, CF
By Anonymous on 08/21/2019
Application: In vitro bioactivity in cell culture