Recombinant Human GPVI Fc Chimera Protein, CF

R&D Systems | Catalog # 10452-GP

HEK293 Expressed
R&D Systems
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Key Product Details

  • R&D Systems HEK293-derived Recombinant Human GPVI Fc Chimera Protein (10452-GP)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

Q9HCN6.4

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all GPVI Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human GPVI protein
Human GPVI
(Gln21-Lys267)
Accession # Q9HCN6.4		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln21

Predicted Molecular Mass

54 kDa

SDS-PAGE

68-76 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Bovine Collagen I is immobilized at 10 µg/mL (100 μL/well), Recombinant Human GPVI Fc Chimera (Catalog # 10452-GP) binds with an ED50 of 0.04-0.36 μg/mL.

Reviewed Applications

Read 1 review rated 4 using 10452-GP in the following applications:

Scientific Data Images for Recombinant Human GPVI Fc Chimera Protein, CF

Recombinant Human GPVI Fc Chimera Protein Binding Activity

Recombinant Human GPVI Fc Chimera Protein Binding Activity

When Bovine Collagen I is immobilized at 10 μg/mL (100 μL/well), Recombinant Human GPVI Fc Chimera (10452-GP) binds with an ED50 of 0.04-0.36 μg/mL.
Recombinant Human GPVI Fc Chimera Protein SDS-PAGE

Recombinant Human GPVI Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human GPVI Fc Chimera Protein (Catalog # 10452-GP) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 68-80 kDa and 136-160 kDa, respectively.

Formulation, Preparation, and Storage

10452-GP
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GPVI

Glycoprotein VI (GPVI) is a 63 kDa platelet/megakaryocyte-specific type I transmembrane glycoprotein of the immunoglobulin superfamily that is an important collagen receptor and initiator of platelet activation, aggregation and thrombin generation (1, 2). GPVI is also a secondary receptor required for platelet spreading on laminin (3). Human GPVI contains a 20 amino acid (aa) signal sequence, a 247 aa extracellular domain (ECD) that has two C-type Ig-like domains followed by a mucin-like, presumably O-glycosylated Ser-Thr-rich region, a 21 aa transmembrane (TM) domain and a 51 aa cytoplasmic tail that contains calmodulin-binding and SH3 domains. Human GPVI ECD shows 69%, 65% and 70% aa identity with mouse, bovine and canine GPVI ECD, respectively. Two splice variants exist; one is 17 aa shorter in the ECD, while the other diverges at aa 260, creating an inactive monomeric and presumably secreted 681 aa protein (3).GPVI associates with the FcR gamma via charged amino acid in the TM domains of GPVI (arginine) and the FcR gamma (aspartic acid) (2). Collagen binding by the GPVI Ig-like domains initiates signaling through the FcR gamma ITAM sequence (2). Dimerization of GPVI (2:2 with FcR gamma ) and N-glycosylation greatly enhances collagen binding (5, 6). Type I and III collagens are strong thrombus-forming components in the vascular subendothelium and atherosclerotic plaques (7). GPVI initiates binding to fibrillar collagens under flow conditions, then activates integrin alpha 2 beta 1 which binds collagen more tightly (8). GPVI deficiencies cause only a mild bleeding tendency, probably because integrin alpha 2 beta 1 is able to minimally initiate collagen binding (8). Normal human GPVI concentration can vary widely and affect maximum thrombin generation (9). Engagement of GPVI by collagens or other agonists, including autoantibodies, causes calmodulin-regulated metalloproteinase cleavage of the 57 kDa ECD and depletes surface GPVI (10).

References

  1. Jandrot-Perrus, M. et al. (2000) Blood 96:1798.
  2. Moroi, M. and S. M. Jung (2004) Thromb. Res. 114:221.
  3. Inoue, O. et al. (2006) Blood 107:1405.
  4. Ezumi, Y. et al. (2000) Biochem. Biophys. Res. Comm. 277:27.
  5. Horii, K. et al. (2006) Blood 108:936.
  6. Kunicki, T. J. et al. (2005) Blood 106:2744.
  7. Cosemans, J. M. et al. (2005) Atherosclerosis 181:19.
  8. Lecut, C. et al. (2005) Thromb. Haemost. 94:107.
  9. Furihata, K. et al. (2001) Arterioscler. Thromb. Vasc. Biol. 21:1857.
  10. Stephens, G. et al. (2005) Blood 105:186.

Long Name

Glycoprotein VI [Platelet]

Alternate Names

GP6

Entrez Gene IDs

51206 (Human); 243816 (Mouse)

Gene Symbol

GP6

UniProt

Q9HCN6.4

Additional GPVI Products

Product Documents for Recombinant Human GPVI Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human GPVI Fc Chimera Protein, CF

For research use only

Related Research Areas

Coagulation

Citations for Recombinant Human GPVI Fc Chimera Protein, CF

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    Recombinant Human GPVI Fc Chimera Protein, CF 10452-GP

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Associated Pathways

Blood Coagulation Signaling Pathways Blood Coagulation Signaling Pathway Thumbnail