Recombinant Human GST-KEAP1 Protein, CF

Catalog # Availability Size / Price Qty
E3-310-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human GST-KEAP1 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Typical protein concentration for use in vitro will depend on experimental conditions.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Keap1 protein
Contains an N-terminal GST (glutathione S-transferase) tag
Accession #
Predicted Molecular Mass
96 kDa

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E3-310

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E3-310

Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol, 5 mM DTT.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 48 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
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Background: Keap1

Kelch-like ECH-associated protein (KEAP1) is the substrate recognition subunit of a BCR (BTB-CUL3-RBX) E3 ubiquitin ligase complex.  With a predicted molecular weight of 70 kDa, human KEAP1 IS 74% identical to its mouse and rat orthologues.  KEAP1 contains an N-terminal BB Domain that mediates interaction with CUL3, a central BACK Domain that plays a role in protein-protein interactions, and six C-terminal Kelch Domains that function in substrate recognition.  KEAP1 is an important sensor of oxidative and electrophilic stress.  Normally the KEAP1/CUL3/RBX1 complex ubiquitinates and causes degradation of NRF2, a transcription factor regulating expression of many cytoprotective genes.  Oxidative stress results in the inability of KEAP1 to ubiquitinate NRF2, leading to the latter proteins accumulation and subsequent upregulation of genes involved in cellular oxidative stress responses.  This recombinant protein (UniProt Q14145-1) contains an N-terminal GST tag.

References
  1. Eggler, A.L. et al. (2009) Biochem. J.  doi:10.1042/BJ20090471
  2. Eggler, A.L. et al. (2005) Proc. Natl. Acad. Sci.  doi: 10.1073/pnas.0502402102
  3. Mills, E.L. et al. (2018) Nature   doi: 10.1038/nature25986
  4. Wei, J. et al. (2021) JACS   doi: 10.1021/jacs.1c04841
Long Name
Kelch-like ECH-associated Protein 1
Entrez Gene IDs
9817 (Human)
Alternate Names
INrf2; INrf2MGC9454; Keap1; kelch-like ECH-associated protein 1; Kelch-like protein 19; KIAA0132MGC4407; KLHL19Cytosolic inhibitor of Nrf2; MGC10630; MGC1114; MGC20887

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