Recombinant Human GST-KEAP1 Protein, CF

R&D Systems | Catalog # E3-310

Will be discontinued when existing inventory is gone.
R&D Systems

Key Product Details

Source

Sf 21 (baculovirus)

Accession Number

Q14145.2

Applications

Bioactivity
View all Keap1 Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Keap1 protein
Met1 - Cys624 with a N-terminal GST (glutathione S-transferase) tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

96 kDa

Activity

Typical protein concentration for use in vitro will depend on experimental conditions.

Formulation, Preparation, and Storage

E3-310
Formulation Supplied as a solution in HEPES, NaCl, Glycerol and DTT.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Keap1

Kelch-like ECH-associated protein (KEAP1) is the substrate recognition subunit of a BCR (BTB-CUL3-RBX) E3 ubiquitin ligase complex.  With a predicted molecular weight of 70 kDa, human KEAP1 IS 74% identical to its mouse and rat orthologues.  KEAP1 contains an N-terminal BB Domain that mediates interaction with CUL3, a central BACK Domain that plays a role in protein-protein interactions, and six C-terminal Kelch Domains that function in substrate recognition.  KEAP1 is an important sensor of oxidative and electrophilic stress.  Normally the KEAP1/CUL3/RBX1 complex ubiquitinates and causes degradation of NRF2, a transcription factor regulating expression of many cytoprotective genes.  Oxidative stress results in the inability of KEAP1 to ubiquitinate NRF2, leading to the latter proteins accumulation and subsequent upregulation of genes involved in cellular oxidative stress responses.  

References

  1. Eggler, A.L. et al. (2009) Biochem. J. 422:171.
  2. Eggler, A.L. et al. (2005) Proc. Natl. Acad. Sci. 102:10070.
  3. Mills, E.L. et al. (2018) Nature  556:113. 
  4. Wei, J. et al. (2021) J. Am. Chem. Soc. 143:15073.   

Long Name

Kelch-like ECH-associated Protein 1

Alternate Names

INrf2

Entrez Gene IDs

9817 (Human)

Gene Symbol

KEAP1

UniProt

Q14145.2

Additional Keap1 Products

Product Documents for Recombinant Human GST-KEAP1 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human GST-KEAP1 Protein, CF

For research use only

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