Recombinant Human GST-UbcH5c/UBE2D3 Protein, CF Summary
Contains an N-terminal GST (glutathione S-transferase) tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||X mg/ml (X μM) in 50 mM HEPES pH 7.0, 200 mM NaCl, 10% Glycerol, 1 mM TCEP|
|Shipping||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Ubiquitin-conjugating Enzyme H5c (UbcH5c), also known as Ubiquitin-conjugating Enzyme E2D 3 (UBE2D3), is a member of the yeast Ubc4/5 family of Ubiquitin-conjugating (E2) enzymes. Human UbcH5c/UBE2D3 has a predicted molecular weight of 17 kDa and shares 88% and 89% amino acid sequence identity with the related family members, UbcH5a and UbcH5b, respectively. In combination with Ubiquitin ligases (E3s) such as CHIP, UbcH5c/UBE2D3 mediates the ubiquitination and subsequent degradation of several regulatory proteins (1). For instance, UbcH5c/UBE2D3 is involved in the poly-ubiquitination and proteasome-mediated degradation of the Nuclear Factor kappaB (NF-kappaB) inhibitor, IkappaB-alpha, and is implicated in NF-kappaB-dependent inflammation (2-4). UbcH5c/UBE2D3 also mediates the ubiquitination of Histone H2A and PCNA, suggesting that it functions during transcriptional regulation, DNA replication, and DNA damage responses (5-7).
- Page, R.C. et al. (2012) Biochemistry 51:4175.
- Gonen, H. et al. (1999) J. Biol. Chem. 274:14823.
- Shembade, N. et al. (2010) Science 327:1135.
- Xia, Z.P. et al. (2009) Nature 461:114.
- Zhang, S. et al. (2008) Cell Cycle 7:3399.
- Bentley, M.L. et al. (2011) EMBO J. 30:3285.
- Polanowska, J. et al. (2006) EMBO J. 25:2178.
Citations for Recombinant Human GST-UbcH5c/UBE2D3 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Sec16A, a key protein in COPII vesicle formation, regulates the stability and localization of the novel ubiquitin ligase RNF183
Authors: Y Wu, XP Guo, S Kanemoto, Y Maeoka, A Saito, R Asada, K Matsuhisa, Y Ohtake, K Imaizumi, M Kaneko
PLoS ONE, 2018;13(1):e0190407.
Genome-wide identification and gene expression profiling of ubiquitin ligases for endoplasmic reticulum protein degradation
Sci Rep, 2016;6(0):30955.
Sample Types: Protein
Applications: Enzyme Assay
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