Ubiquitin-conjugating Enzyme H5c (UbcH5c), also known as Ubiquitin-conjugating Enzyme E2D 3 (UBE2D3), is a member of the yeast Ubc4/5 family of Ubiquitin-conjugating (E2) enzymes. Human UbcH5c/UBE2D3 has a predicted molecular weight of 17 kDa and shares 88% and 89% amino acid sequence identity with the related family members, UbcH5a and UbcH5b, respectively. In combination with Ubiquitin ligases (E3s) such as CHIP, UbcH5c/UBE2D3 mediates the ubiquitination and subsequent degradation of several regulatory proteins (1). For instance, UbcH5c/UBE2D3 is involved in the poly-ubiquitination and proteasome-mediated degradation of the Nuclear Factor kappaB (NF-kappaB) inhibitor, IkappaB-alpha, and is implicated in NF-kappaB-dependent inflammation (2-4). UbcH5c/UBE2D3 also mediates the ubiquitination of Histone H2A and PCNA, suggesting that it functions during transcriptional regulation, DNA replication, and DNA damage responses (5-7).
Recombinant Human GST-UbcH5c/UBE2D3 Protein, CF
R&D Systems | Catalog # E2-625
Discontinued Product
E2-625 has been discontinued.
View all UbcH5c/UBE2D3 products.
Key Product Details
Source
E. coli
Accession Number
Applications
Enzyme Activity
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Product Specifications
Source
E. coli-derived human UbcH5c/UBE2D3 protein
Contains an N-terminal GST (glutathione S-transferase) tag
Contains an N-terminal GST (glutathione S-transferase) tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Predicted Molecular Mass
44 kDa
Activity
Recombinant Human GST-UbcH5c/UBE2D3 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human GST-UbcH5c/UBE2D3 concentration of 0.1-1 μM.
Reviewed Applications
Read 2 reviews rated 4 using E2-625 in the following applications:
Formulation, Preparation, and Storage
E2-625
| Formulation | X mg/ml (X μM) in 50 mM HEPES pH 7.0, 200 mM NaCl, 10% Glycerol, 1 mM TCEP |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: UbcH5c/UBE2D3
References
- Page, R.C. et al. (2012) Biochemistry 51:4175.
- Gonen, H. et al. (1999) J. Biol. Chem. 274:14823.
- Shembade, N. et al. (2010) Science 327:1135.
- Xia, Z.P. et al. (2009) Nature 461:114.
- Zhang, S. et al. (2008) Cell Cycle 7:3399.
- Bentley, M.L. et al. (2011) EMBO J. 30:3285.
- Polanowska, J. et al. (2006) EMBO J. 25:2178.
Alternate Names
UBC5C, UBE2D3
Gene Symbol
UBE2D3
UniProt
Additional UbcH5c/UBE2D3 Products
Product Documents for Recombinant Human GST-UbcH5c/UBE2D3 Protein, CF
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human GST-UbcH5c/UBE2D3 Protein, CF
For research use only
Citations for Recombinant Human GST-UbcH5c/UBE2D3 Protein, CF
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Customer Reviews for Recombinant Human GST-UbcH5c/UBE2D3 Protein, CF (2)
4 out of 5
2 Customer Ratings
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Application: Binding assay/Protein-protein interactionVerified Customer | Posted 09/10/2018
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