Recombinant Human GST-UbcH5c/UBE2D3 Protein, CF

Catalog # Availability Size / Price Qty
E2-625-100
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (2)
FAQs
Reviews (2)

Recombinant Human GST-UbcH5c/UBE2D3 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
Recombinant Human GST-UbcH5c/UBE2D3 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human GST-UbcH5c/UBE2D3 concentration of 0.1-1 μM.
Source
E. coli-derived human UbcH5c/UBE2D3 protein
Contains an N-terminal GST (glutathione S-transferase) tag
Accession #
Predicted Molecular Mass
44 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E2-625

Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.0, 200 mM NaCl, 10% Glycerol, 1 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

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Background: UbcH5c/UBE2D3

Ubiquitin-conjugating Enzyme H5c (UbcH5c), also known as Ubiquitin-conjugating Enzyme E2D 3 (UBE2D3), is a member of the yeast Ubc4/5 family of Ubiquitin-conjugating (E2) enzymes. Human UbcH5c/UBE2D3 has a predicted molecular weight of 17 kDa and shares 88% and 89% amino acid sequence identity with the related family members, UbcH5a and UbcH5b, respectively. In combination with Ubiquitin ligases (E3s) such as CHIP, UbcH5c/UBE2D3 mediates the ubiquitination and subsequent degradation of several regulatory proteins (1). For instance, UbcH5c/UBE2D3 is involved in the poly-ubiquitination and proteasome-mediated degradation of the Nuclear Factor kappaB (NF-kappaB) inhibitor, IkappaB-alpha, and is implicated in NF-kappaB-dependent inflammation (2-4). UbcH5c/UBE2D3 also mediates the ubiquitination of Histone H2A and PCNA, suggesting that it functions during transcriptional regulation, DNA replication, and DNA damage responses (5-7).

References
  1. Page, R.C. et al. (2012) Biochemistry 51:4175.
  2. Gonen, H. et al. (1999) J. Biol. Chem. 274:14823.
  3. Shembade, N. et al. (2010) Science 327:1135.
  4. Xia, Z.P. et al. (2009) Nature 461:114.
  5. Zhang, S. et al. (2008) Cell Cycle 7:3399.
  6. Bentley, M.L. et al. (2011) EMBO J. 30:3285.
  7. Polanowska, J. et al. (2006) EMBO J. 25:2178. 
Entrez Gene IDs
7323 (Human); 66105 (Mouse); 81920 (Rat)
Alternate Names
E2(17)KB 3; E2(17)KB3; EC 6.3.2.19; MGC43926; MGC5416; UBC4/5; UBC5C; UbcH5c; UBE2D3; Ubiquitin carrier protein D3; ubiquitin-conjugating enzyme E2 D3; Ubiquitin-conjugating enzyme E2(17)KB 3; Ubiquitin-conjugating enzyme E2-17 kDa 3; ubiquitin-conjugating enzyme E2D 3 (homologous to yeast UBC4/5); ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog, yeast); Ubiquitin-protein ligase D3

Citations for Recombinant Human GST-UbcH5c/UBE2D3 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Sec16A, a key protein in COPII vesicle formation, regulates the stability and localization of the novel ubiquitin ligase RNF183
    Authors: Y Wu, XP Guo, S Kanemoto, Y Maeoka, A Saito, R Asada, K Matsuhisa, Y Ohtake, K Imaizumi, M Kaneko
    PLoS ONE, 2018;13(1):e0190407.
    Applications: Bioassay
  2. Genome-wide identification and gene expression profiling of ubiquitin ligases for endoplasmic reticulum protein degradation
    Sci Rep, 2016;6(0):30955.
    Species: Human
    Sample Types: Protein
    Applications: Enzyme Assay

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Reviews for Recombinant Human GST-UbcH5c/UBE2D3 Protein, CF

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Recombinant Human GST-UbcH5c/UBE2D3 Protein, CF
By Anonymous on 09/10/2018
Application: Binding assay/Protein-protein interaction

Recombinant Human GST-UbcH5c/UBE2D3 Protein, CF
By Anonymous on 09/10/2018
Application: Enzymatic activity in vitro