Recombinant Human Hip Protein, CF Summary
ED50 = 0.1-0.6 µg/mL.
Accession # Q96QV1
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Recombinant Human Sonic Hedgehog/Shh (Catalog # 8908-SH) induces alkaline phosphatase production in C3H10T1/2 cells (green line). Recombinant Human Hip (Catalog # 9280-HP) inhibits a constant dose of 20 ng/mL of Recombinant Human Sonic Hedgehog/Shh Protein, High Activity (Catalog # 8908-SH) in C3H10T1/2 mouse embryonic fibroblasts with an ED50 = 0.1-0.6 µg/mL (orange line).
Hedgehog signaling proteins act as mitogens, morphogens, or inducing factors in many different cell types during embryonic development. They aid in growth, patterning, and morphogenesis in both vertebrates and insects (1). Hip (Hedgehog-interacting protein) is a type I transmembrane protein identified for its ability to bind biologically active Sonic Hedgehog. It is comprised of 700 amino acids (aa), and includes a hydrophobic signal sequence, two EGF-like domains near the C-terminus, and a 22 aa membrane-attachment helix at the C-terminal end (2, 3). Mouse and human Hip share 94% aa sequence identity through the entire protein sequence (4). Alternative splicing generates a soluble isoform that lacks the C-terminal half of the protein (5). Hip has only been identified in vertebrates and binds all three mammalian Hedgehogs: sonic (Shh), desert (Dhh), and Indian (Ihh). Like the Hedgehog receptor Patched, Hip is a transcriptional target of Hedgehog signaling (2). Unlike Patched, Hip's ability to bind hedgehogs is not involved in transducing a signal intracellularly, rather it regulates the availability of Hedgehog ligand extracellularly (6). Transgenic mice overexpressing Hip in proliferating chondrocytes display skeletal defects similar to those observed in Ihh mutant mice. These results indicate that Hip is involved in attenuating Hedgehog signaling (2). The expression pattern of Hip correlates with its ability to interact with all three mammalian Hedgehogs. It is expressed in a variety of organs, adjacent with sites of hedgehog expression. For instance, Shh is expressed in the epithelium of the lung, and Hip is found in the underlying lung mesenchyme (2). In fact, Hip knock-out mice exhibit neonatal lethality with respiratory failure due to defective branching morphogenesis. This phenotype correlates with altered expression of Shh markers suggesting an increase in Shh signaling (6). Interestingly, other developmental mechanisms that rely on normal Shh signaling, such as dorsal-ventral patterning of the neural tube, development of the somites, and organ laterality appeared histologically normal in Hip-/- mice (6). Hip is also expressed in vascular endothelial cells but is down-regulated during angiogenesis and in various tumors (7, 8).
- Ramsbottom, S.A. and M.E. Pownall (2016) J. Dev. Biol. 4:23.
- Chuang, P-T. and A.P. McMahon (1999) Nature 397:617.
- Bishop, B. et al. (2009) Nat. Struct. Mol. Biol. 16:698.
- Bak, M. et al. (2001) Cytogenet. Cell Genet. 92:300.
- Coulombe, J. et al. (2004) Mol. Cell. Neurosci. 25:323.
- Chuang, P-T. et al. (2003) Genes Dev. 17:342.
- Olsen, C.L. et al. (2004) BMC Cancer 4:43.
- Tada, M. et al. (2008) Clin. Cancer Res. 14:3768.
Citations for Recombinant Human Hip Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
Filter your results:
Human alveolar type 2 epithelium transdifferentiates into metaplastic KRT5+ basal cells
Authors: JJ Kathiriya, C Wang, M Zhou, A Brumwell, M Cassandras, CJ Le Saux, M Cohen, KD Alysandrat, B Wang, P Wolters, M Matthay, DN Kotton, HA Chapman, T Peng
Nature Cell Biology, 2021;0(0):.
Sample Types: Organoid
Hedgehog Interacting Protein Promotes Fibrosis and Apoptosis in Glomerular Endothelial Cells in Murine Diabetes
Authors: XP Zhao, SY Chang, MC Liao, CS Lo, I Chenier, H Luo, JL Chiasson, JR Ingelfinge, JSD Chan, SL Zhang
Sci Rep, 2018;8(1):5958.
Sample Types: Whole Cells
No product specific FAQs exist for this product, however you mayView all Proteins and Enzyme FAQs
Reviews for Recombinant Human Hip Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Human Hip Protein, CF and earn rewards!
Have you used Recombinant Human Hip Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image