Recombinant Human Hip Protein, CF

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Recombinant Human Hip Protein Bioactivity
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Product Details
Citations (2)
Supplemental Products

Recombinant Human Hip Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its ability to inhibit Sonic Hedgehog (Shh) induction of alkaline phosphatase production in C3H10T1/2 mouse embryonic fibroblast cells. Recombinant Human Hip (Catalog # 9280-HP) inhibits a constant dose of 20 ng/mL of Recombinant Human Sonic Hedgehog/Shh Protein, High Activity (Catalog # 8908-SH) in a C3H10T1/2 mouse embryonic fibroblast cell alkaline phosphatase induction assay with an
ED50 = 0.1-0.6 µg/mL.
Chinese Hamster Ovary cell line, CHO-derived human Hip protein
Human Hip
Accession # Q96QV1
Accession #
N-terminal Sequence
Predicted Molecular Mass
75 kDa
77-89 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Human Hip Protein Bioactivity View Larger

Recombinant Human Sonic Hedgehog/Shh (Catalog # 8908-SH) induces alkaline phosphatase production in C3H10T1/2 cells (green line). Recombinant Human Hip (Catalog # 9280-HP) inhibits a constant dose of 20 ng/mL of Recombinant Human Sonic Hedgehog/Shh Protein, High Activity (Catalog # 8908-SH) in C3H10T1/2 mouse embryonic fibroblasts with an ED50 = 0.1-0.6 µg/mL (orange line).

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Background: Hip

Hedgehog signaling proteins act as mitogens, morphogens, or inducing factors in many different cell types during embryonic development. They aid in growth, patterning, and morphogenesis in both vertebrates and insects (1). Hip (Hedgehog-interacting protein) is a type I transmembrane protein identified for its ability to bind biologically active Sonic Hedgehog. It is comprised of 700 amino acids (aa), and includes a hydrophobic signal sequence, two EGF-like domains near the C-terminus, and a 22 aa membrane-attachment helix at the C-terminal end (2, 3). Mouse and human Hip share 94% aa sequence identity through the entire protein sequence (4). Alternative splicing generates a soluble isoform that lacks the C-terminal half of the protein (5). Hip has only been identified in vertebrates and binds all three mammalian Hedgehogs: sonic (Shh), desert (Dhh), and Indian (Ihh). Like the Hedgehog receptor Patched, Hip is a transcriptional target of Hedgehog signaling (2). Unlike Patched, Hip's ability to bind hedgehogs is not involved in transducing a signal intracellularly, rather it regulates the availability of Hedgehog ligand extracellularly (6). Transgenic mice overexpressing Hip in proliferating chondrocytes display skeletal defects similar to those observed in Ihh mutant mice. These results indicate that Hip is involved in attenuating Hedgehog signaling (2). The expression pattern of Hip correlates with its ability to interact with all three mammalian Hedgehogs. It is expressed in a variety of organs, adjacent with sites of hedgehog expression. For instance, Shh is expressed in the epithelium of the lung, and Hip is found in the underlying lung mesenchyme (2). In fact, Hip knock-out mice exhibit neonatal lethality with respiratory failure due to defective branching morphogenesis. This phenotype correlates with altered expression of Shh markers suggesting an increase in Shh signaling (6). Interestingly, other developmental mechanisms that rely on normal Shh signaling, such as dorsal-ventral patterning of the neural tube, development of the somites, and organ laterality appeared histologically normal in Hip-/- mice (6). Hip is also expressed in vascular endothelial cells but is down-regulated during angiogenesis and in various tumors (7, 8).

  1. Ramsbottom, S.A. and M.E. Pownall (2016) J. Dev. Biol. 4:23.
  2. Chuang, P-T. and A.P. McMahon (1999) Nature 397:617.
  3. Bishop, B. et al. (2009) Nat. Struct. Mol. Biol. 16:698.
  4. Bak, M. et al. (2001) Cytogenet. Cell Genet. 92:300.
  5. Coulombe, J. et al. (2004) Mol. Cell. Neurosci. 25:323.
  6. Chuang, P-T. et al. (2003) Genes  Dev. 17:342.
  7. Olsen, C.L. et al. (2004) BMC Cancer 4:43.
  8. Tada, M. et al. (2008) Clin. Cancer Res. 14:3768.
Long Name
Hedgehog-interacting Protein
Entrez Gene IDs
64399 (Human); 15245 (Mouse)
Alternate Names
FLJ90230; hedgehog interacting protein; hedgehog-interacting protein; HHIP; Hip; HIPFLJ20992

Citations for Recombinant Human Hip Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Human alveolar type 2 epithelium transdifferentiates into metaplastic KRT5+ basal cells
    Authors: JJ Kathiriya, C Wang, M Zhou, A Brumwell, M Cassandras, CJ Le Saux, M Cohen, KD Alysandrat, B Wang, P Wolters, M Matthay, DN Kotton, HA Chapman, T Peng
    Nature Cell Biology, 2021;0(0):.
    Species: Human
    Sample Types: Organoid
    Applications: Bioassay
  2. Hedgehog Interacting Protein Promotes Fibrosis and Apoptosis in Glomerular Endothelial Cells in Murine Diabetes
    Authors: XP Zhao, SY Chang, MC Liao, CS Lo, I Chenier, H Luo, JL Chiasson, JR Ingelfinge, JSD Chan, SL Zhang
    Sci Rep, 2018;8(1):5958.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay


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