Recombinant Human His10-FLAG-BRD4 (49-460) Protein, CF Summary
Glu49 - Glu460
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol, 1 mM DTT
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
1 μg per lane of Recombinant Human His10-FLAG-BRD4 (49-460) (Catalog # SP-600) was resolved with SDS-PAGE under reducing conditions and visualized by colloidal Coomassie Blue staining, showing a band at 57 kDa.
Bromodomain-containing protein 4 (BRD4) is a member of the BET class chromatin reader proteins that bind acetylated histones and play a key role in transcriptional regulation and transmission of epigenetic memory. BRD4 has two N-terminal bromodomains and one NET (N-terminal Extra Terminal) domain. BRD bromodomains serve as recognition motifs for acetylated lysine residues on histones, while the NET domain may function by promoting phosphorylation of the C-terminal domain (CTD) of RNA Polymerase II. BRD4 is a potential therapeutic target in many diseases including breast cancer, AML, multiple myeloma, colon cancer and others. This recombinant protein contains an N-terminal portion of BRD4 including both bromodomains and contains both His10 and FLAG tags at the N-terminus. This protein is useful as a substrate in in vitro assays using recombinant E3 Ubiquitin ligases and small molecule degraders.
- Devaiah B.N. et al. (2012) Proc. Natl. Acad. Sci. 109: 6927
- Gadd M.S. et al. (2017) Nat. Chem. Biol. 13: 514
- Wang R. et al. (2012) J Biol. Chem. 287: 10738
- Zhang W. et al. (1994) J Biol. Chem. 287: 43137
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