Recombinant Human His10-FLAG-BRD4 (49-460) Protein, CF

Catalog # Availability Size / Price Qty
SP-600-100
Recombinant Human His10-FLAG-BRD4 (49-460) Protein, CF SDS-PAGE
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Recombinant Human His10-FLAG-BRD4 (49-460) Protein, CF Summary

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Source
E. coli-derived human BRD4 protein
Glu49 - Glu460

Accession #
Predicted Molecular Mass
52 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

SP-600

Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol, 1 mM DTT
Shipping
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 60 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Data Image

SDS-PAGE SDS-PAGE View Larger

1  μg per lane of Recombinant Human His10-FLAG-BRD4 (49-460) (Catalog # SP-600) was resolved with SDS-PAGE under reducing conditions and visualized by colloidal Coomassie Blue staining, showing a band at 57 kDa. 

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Background: BRD4

Bromodomain-containing protein 4 (BRD4) is a member of the BET class chromatin reader proteins that bind acetylated histones and play a key role in transcriptional regulation and transmission of epigenetic memory.  BRD4 has two N-terminal bromodomains and one NET (N-terminal Extra Terminal) domain.  BRD bromodomains serve as recognition motifs for acetylated lysine residues on histones, while the NET domain may function by promoting phosphorylation of the C-terminal domain (CTD) of RNA Polymerase II.  BRD4 is a potential therapeutic target in many diseases including breast cancer, AML, multiple myeloma, colon cancer and others.  This recombinant protein contains an N-terminal portion of BRD4 including both bromodomains and contains both His10 and FLAG tags at the N-terminus.  This protein is useful as a substrate in in vitro assays using recombinant E3 Ubiquitin ligases and small molecule degraders.

References
  1. Devaiah B.N. et al. (2012) Proc. Natl. Acad. Sci.  109: 6927
  2. Gadd M.S. et al. (2017) Nat. Chem. Biol.  13: 514
  3. Wang R. et al. (2012) J Biol. Chem.  287: 10738
  4. Zhang W. et al. (1994) J Biol. Chem.  287: 43137
Long Name
Bromodomain Containing 4
Entrez Gene IDs
23476 (Human); 57261 (Mouse)
Alternate Names
BRD4; bromodomain containing 4; bromodomain-containing 4; CAPchromosome-associated protein; HUNK1; HUNK1bromodomain-containing protein 4; HUNKI; MCAP; Protein HUNK1

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