Home / ATG3 / Recombinant Human His6-ATG3 Protein, CF

Recombinant Human His6-ATG3 Protein, CF

Catalog #: E2-670 Datasheet / COA / SDS

Discontinued Product

E2-670 has been discontinued.
View all ATG3 products.
R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
FAQs
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human His6-ATG3 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
Recombinant Human His6-Apg3p/ATG3/Apg3L is a member of the Apg-conjugating (E2) enzyme family that receives Apg from a Apg-activating (E1) enzyme and subsequently interacts with a Apg ligase (E3) to conjugate Apg to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-Apg3p/ATG3/Apg3L concentration of 0.1-1 μM.
Source
E. coli-derived human ATG3 protein
Contains a C-terminal 6-His tag
Accession #
NP_071933
Predicted Molecular Mass
37 kDa

Product Datasheets

You must select a language.

x

E2-670

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E2-670

Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP 
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: ATG3

Autophagy-related 3 (ATG3), also known as Apg3L and Apg3p, functions as a regulatory component of autophagosome biogenesis necessary for autophagy. It is a 314 amino acid (aa) protein with a predicted molecular weight of 36 kDa. ATG3 exhibits 98% aa sequence identity with both its mouse and rat orthologs. It is a Ubiquitin-conjugating (E2)-like enzyme that works in concert with Atg12-Atg5, a Ubiquitin Ligase (E3)-like enzyme conjugate, to catalyze the transfer of Atg8 (LC3, GATE-16, and GABARAP in mammals) to phosphatidylethanolamine (PE) in the developing autophagosome membrane (1-4). This lipidation of Atg8 proteins is necessary for autophagosome maturation (5).

References
  1. Ichimura, Y. et al. (2000) Nature 408:488.
  2. Ichimura, T. et al. (2004) J. Biol. Chem. 279:40584.
  3. Yamaguchi, M. et al. (2010) J. Biol. Chem. 285:29599.
  4. Otomo, C. et al. (2013) Nat. Struct. Mol. Biol. 20:59.
  5. Shpilka, T. et al. (2012) Genome Biol. 12:226. 
Long Name
Autophagy Related 3 Homolog
Entrez Gene IDs
64422 (Human); 67841 (Mouse); 171415 (Rat)
Alternate Names
APG3 autophagy 3-like (S. cerevisiae); APG3; APG3L; APG3-LIKE; Apg3p; ATG3 autophagy related 3 homolog (S. cerevisiae); ATG3; Autophagy-related protein 3; DKFZp564M1178; EC 6.3.2.-; FLJ22125,2610016C12Rik; hApg3; MGC15201; PC3-96; Protein PC3-96; ubiquitin-like-conjugating enzyme ATG3

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human His6-ATG3 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human His6-ATG3 Protein, CF and earn rewards!

Have you used Recombinant Human His6-ATG3 Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review