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Recombinant Human His6-ATG4B Protein, CF

Catalog #: E-400
1 Citation Datasheet / COA / SDS
 

Discontinued Product

E-400 has been discontinued.
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Recombinant Human His6-ATG4B Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
Recombinant Human His6-Apg4b/ATG4B is a Apg8-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-Apg4b/ATG4B concentration of 10-100 nM. A 15 minute pre-incubation with 10 mM DTT is recommended to achieve maximum activity.
Source
E. coli-derived human ATG4B protein
Contains an N-terminal 6-His tag
Accession #
Q9Y4P1
Predicted Molecular Mass
46 kDa

Product Datasheets

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E-400

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-400

Formulation

X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 1 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ATG4B

Autophagy related protein 4 homolog B (ATG4B), also known as Autophagy-related Protein 4b (Apg4b) is a cysteine protease and a member of the ATG4 protein family (ATG4A- ATG4D). It is a 393 amino acid (aa) protein with a predicted molecular weight of 44 kDa. Human ATG4B/Apg4b shares 95% aa sequence identity with its mouse ortholog. Members of the ATG4 family cleave the C-terminal sequences of the precursor forms of the Autophagy-related Protein 8 (Atg8) family of proteins, including GABARAP, GATE-16, and MAP-LC3 (1). This cleavage exposes the conserved C-terminal glycine that is required for conjugation to phosphatidylethanolamine (PE) in the phagophore membrane and is necessary for autophagosome maturation (2). Although some ATG4 family members exhibit specificity for certain Atg8 proteins in vitro, ATG4B/Apg4b appears to be relatively non-selective (3).

References
  1. Till, A. & S. Subramani (2010) J. Clin. Invest. 120:2273
  2. Shpilka, T. et al. (2011) Genome Biol. 12:226.
  3. Li, M. et al. (2011) J. Biol. Chem. 286:7327.
Long Name
ATG4 Autophagy Related 4 Homolog B
Entrez Gene IDs
23192 (Human); 66615 (Mouse); 316640 (Rat)
Alternate Names
APG4 autophagy 4 homolog B (S. cerevisiae); APG4 autophagy 4 homolog B; Apg4B; ATG4 autophagy related 4 homolog B (S. cerevisiae); ATG4B; AUTL1; AUTL1MGC1353; AUT-like 1 cysteine endopeptidase; autophagin-1; Autophagy-related cysteine endopeptidase 1; Autophagy-related protein 4 homolog B; cysteine protease ATG4B; DKFZp586D1822; EC 3.4.22; EC 3.4.22.-; hAPG4B; KIAA0943

Citation for Recombinant Human His6-ATG4B Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. The carboxyl-terminal amino acids render pro-human LC3B migration similar to lipidated LC3B in SDS-PAGE.
    Authors: Wang, Wei, Chen, Zhixia, Billiar, Timothy, Stang, Michael, Gao, Wentao
    PLoS ONE, 2013-09-10;8(9):e74222.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay

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