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Recombinant Human His6-ATG7 Isoform 1 Protein, CF

Catalog #: E-318
1 Citation Datasheet / COA / SDS
 

Discontinued Product

E-318 has been discontinued.
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Recombinant Human His6-ATG7 Isoform 1 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
Recombinant Human His6-ATG7 (Apg7) is a member of the Apg-activating (E1) enzyme family that is required for the first step of the enzymatic cascade that subsequently utilizes a Apg-conjugating (E2) enzyme and a Apg ligase (E3) to conjugate Apg to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-ATG7 (Apg7) concentration of 50-200 nM.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human ATG7 protein
Contains an N-terminal 6-His tag
Accession #
O95352
Predicted Molecular Mass
80 kDa

Product Datasheets

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E-318

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-318

Formulation

X mg/ml (X µM) in 50 mM HEPES pH 7.0, 150 mM NaCl, 2 mM TCEP, 10% glycerol
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ATG7

ATG7, also known as APG7, is a 78 kDa cytosolic ubiquitin­E1-­like enzyme that plays a key role in the autophagy pathway of intracellular bulk degradation.  It is required for the conjugation of ATG5 to ATG12, the lipidation of ATG8 proteins including GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A, and the subsequent formation of autophagosomes.  ATG7 is required for mitochondrial removal during erythropoiesis (“mitophagy”) and for the maintenance of axonal homeostasis.  Alternate splicing of human ATG7 generates an isoform that lacks 31 aa at the C-­terminus, a region that is required for ATG8 lipidation. Human ATG7 shares 93% aa sequence identitiy with mouse and rat ATG7.  This recombinant proteins has an N-terminal 6-His tag.

References
  1. Ichimura Y., et al. (2000) Nature 408: 488-492.
  2. Komatsue M., et al. (2001) J. Biol. Chem. 276: 9846-9854.
  3. Mizushima N., et al. (1998) J. Biol. Chem. 273: 33889-33892.
  4. Ohsumi Y., et al. (2001) Nat. Rev.  2: 211-216.
  5. Reggiori F. and Klionsky D.J. (2002)  Euk. Cell.  1:11-21.
  6. Tanida I., et al. (2001) J. Biol. Chem. 276: 1701-1706
  7. Tanida I., et al. (2002) J. Biol. Chem. 277: 13739-13744.
  8. Yu L., et al. (2004) Science 302: 1500-1502.
  9. Yuan W., et al. (1999) Mol. Cell. Biol. 10: 1353-1366
Long Name
ATG7 Autophagy Related 7 Homolog
Entrez Gene IDs
10533 (Human); 74244 (Mouse); 312647 (Rat); 856576 (Yeast)
Alternate Names
APG7 autophagy 7-like (S. cerevisiae); APG7; APG7L; APG7-LIKE; ATG12-activating enzyme E1 ATG7; ATG7 autophagy related 7 homolog (S. cerevisiae); ATG7; Autophagy-related protein 7; DKFZp434N0735; GSA7; hAGP7; ubiquitin activating enzyme E1-like protein; Ubiquitin-activating enzyme E1-like protein; ubiquitin-like modifier-activating enzyme ATG7

Citation for Recombinant Human His6-ATG7 Isoform 1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Oxidation of Atg3 and Atg7 mediates inhibition of autophagy
    Authors: K Frudd, T Burgoyne, JR Burgoyne
    Nat Commun, 2018-01-08;9(1):95.
    Applications: Bioassay

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