Recombinant Human His6-ATG7 Isoform 1 Protein, CF

R&D Systems | Catalog # E-318

 
R&D Systems
Discontinued Product
E-318 has been discontinued. View all ATG7 products.

Key Product Details

Source

Sf 21 (baculovirus)

Accession Number

O95352

Applications

Bioactivity
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Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human ATG7 protein
Contains an N-terminal 6-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

80 kDa

Activity

Recombinant Human His6-ATG7 (Apg7) is a member of the Apg-activating (E1) enzyme family that is required for the first step of the enzymatic cascade that subsequently utilizes a Apg-conjugating (E2) enzyme and a Apg ligase (E3) to conjugate Apg to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-ATG7 (Apg7) concentration of 50-200 nM.

Reviewed Applications

Read 1 review rated 5 using E-318 in the following applications:

Formulation, Preparation, and Storage

E-318
Formulation

X mg/ml (X µM) in 50 mM HEPES pH 7.0, 150 mM NaCl, 2 mM TCEP, 10% glycerol
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: ATG7

ATG7, also known as APG7, is a 78 kDa cytosolic ubiquitin­E1-­like enzyme that plays a key role in the autophagy pathway of intracellular bulk degradation.  It is required for the conjugation of ATG5 to ATG12, the lipidation of ATG8 proteins including GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A, and the subsequent formation of autophagosomes.  ATG7 is required for mitochondrial removal during erythropoiesis (“mitophagy”) and for the maintenance of axonal homeostasis.  Alternate splicing of human ATG7 generates an isoform that lacks 31 aa at the C-­terminus, a region that is required for ATG8 lipidation. Human ATG7 shares 93% aa sequence identitiy with mouse and rat ATG7.  This recombinant proteins has an N-terminal 6-His tag.

References

  1. Ichimura Y., et al. (2000) Nature 408: 488-492.
  2. Komatsue M., et al. (2001) J. Biol. Chem. 276: 9846-9854.
  3. Mizushima N., et al. (1998) J. Biol. Chem. 273: 33889-33892.
  4. Ohsumi Y., et al. (2001) Nat. Rev.  2: 211-216.
  5. Reggiori F. and Klionsky D.J. (2002)  Euk. Cell.  1:11-21.
  6. Tanida I., et al. (2001) J. Biol. Chem. 276: 1701-1706
  7. Tanida I., et al. (2002) J. Biol. Chem. 277: 13739-13744.
  8. Yu L., et al. (2004) Science 302: 1500-1502.
  9. Yuan W., et al. (1999) Mol. Cell. Biol. 10: 1353-1366

Long Name

ATG7 Autophagy Related 7 Homolog

Alternate Names

APG7, APG7L, GSA7

Entrez Gene IDs

10533 (Human); 74244 (Mouse); 312647 (Rat); 856576 (Yeast)

Gene Symbol

ATG7

UniProt

O95352

Additional ATG7 Products

Product Documents for Recombinant Human His6-ATG7 Isoform 1 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human His6-ATG7 Isoform 1 Protein, CF

For research use only

Citations for Recombinant Human His6-ATG7 Isoform 1 Protein, CF

Customer Reviews for Recombinant Human His6-ATG7 Isoform 1 Protein, CF (1)

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  • Recombinant Human His6-ATG7 Isoform 1 Protein, CF
    Name: Anonymous
    Application: In vitro redox reaction.
    Verified Customer | Posted 12/20/2022
    In vitro redox reaction with recombinant Atg7. Atg7 was reduced by dithiothreitol in vitro. Reduced thiols were labeled with biotin labeled iodoacetamide and proved by HRP-streptavidin. Total Atg7 was detected by anti-Atg7 antibody.
    The recombinant Atg7 was reduced by dithiothreitol in vitro. Reduced thiols were labeled with biotin labeled iodoacetamide and proved by HRP-streptavidin.
    Recombinant Human His6-ATG7 Isoform 1 Protein, CF E-318

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