Recombinant Human His6-GABARAP Protein, CF

Catalog # Availability Size / Price Qty
UL-410-500
Product Details
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Recombinant Human His6-GABARAP Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Recombinant Human His6-GABARAP can be conjugated to substrate proteins via the subsequent actions of an ATG-activating (E1) enzyme, an ATG-conjugating (E2) enzyme, and an ATG ligase (E3).  Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-GABARAP concentration of 10-50 μM.
Source
E. coli-derived human GABARAP protein
Contains a 6-His tag
Accession #
Predicted Molecular Mass
19 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

UL-410

Formulation

X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 10% (v/v) Glycerol

Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GABARAP

gamma -Aminobutyric Acid Receptor-associated Protein (GABARAP) is an 117 amino acid (aa) protein with a predicted molecular weight of 14 kDa. It is a member of the GABARAP subfamily of the Autophagy-related 8 (ATG8) family of proteins (1). The GABARAP subfamily also includes GABARAPL1. GABARAP has 100% aa sequence identity with its mouse and rat orthologs and is orthologous to yeast ATG8. ATG8 family members show structural similarity with Ubiquitin, but lack aa sequence similarity. GABARAP was first described for its putative involvement GABA A Receptor trafficking (2,3). However, it is best known for its role in autophagy (1,4). GABARAP covalently attaches to phosphatidylethanolamine (PE) in the phagophore (autophagosome precursor) membrane using a Ubiquitin-like conjugation system that includes Ubiquitin-activating (E1)-, Ubiquitin-conjugating (E2)-, and Ubiquitin Ligase (E3)-like enzymes.  Here it is involved in the later stages of autophagosome maturation (4,5). It may also be involved in cargo recruitment to autophagosomes (1).

References
  1. Shpilka, T. et al. (2011) Genome Biol. 12:226.
  2. Wang, H. et al. (1999) Nature 397:69.
  3. Leil, T.A. et al. (2004) J. Neurosci. 24:11429.
  4. Weidberg, H. et al. (2010) EMBO J. 29:1792.
  5. Weidberg, H. et al. (2011) Ann. Rev. Biochem. 80:125.
Long Name
gamma-Aminobutyric Acid Type A Receptor Associated Protein
Entrez Gene IDs
11337 (Human); 56486 (Mouse); 58974 (Rat)
Alternate Names
Apg8p1; ATG8A; FLC3B; FLJ25768; GABA(A) receptor-associated proteinMGC120154; GABARAP; gamma-aminobutyric acid receptor-associated protein; MM46; MM46MGC120155

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