Recombinant Human His6-GABARAP Protein, CF

R&D Systems | Catalog # UL-410

R&D Systems
Discontinued Product
UL-410 has been discontinued. View all GABARAP products.

Key Product Details

Source

E. coli

Accession Number

NP_009209

Applications

Enzyme Activity
View all GABARAP Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human GABARAP protein
Contains a 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

19 kDa

Activity

Recombinant Human His6-GABARAP can be conjugated to substrate proteins via the subsequent actions of an ATG-activating (E1) enzyme, an ATG-conjugating (E2) enzyme, and an ATG ligase (E3).  Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-GABARAP concentration of 10-50 μM.

Formulation, Preparation, and Storage

UL-410
Formulation

X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 10% (v/v) Glycerol
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: GABARAP

gamma -Aminobutyric Acid Receptor-associated Protein (GABARAP) is an 117 amino acid (aa) protein with a predicted molecular weight of 14 kDa. It is a member of the GABARAP subfamily of the Autophagy-related 8 (ATG8) family of proteins (1). The GABARAP subfamily also includes GABARAPL1. GABARAP has 100% aa sequence identity with its mouse and rat orthologs and is orthologous to yeast ATG8. ATG8 family members show structural similarity with Ubiquitin, but lack aa sequence similarity. GABARAP was first described for its putative involvement GABA A Receptor trafficking (2,3). However, it is best known for its role in autophagy (1,4). GABARAP covalently attaches to phosphatidylethanolamine (PE) in the phagophore (autophagosome precursor) membrane using a Ubiquitin-like conjugation system that includes Ubiquitin-activating (E1)-, Ubiquitin-conjugating (E2)-, and Ubiquitin Ligase (E3)-like enzymes.  Here it is involved in the later stages of autophagosome maturation (4,5). It may also be involved in cargo recruitment to autophagosomes (1).

References

  1. Shpilka, T. et al. (2011) Genome Biol. 12:226.
  2. Wang, H. et al. (1999) Nature 397:69.
  3. Leil, T.A. et al. (2004) J. Neurosci. 24:11429.
  4. Weidberg, H. et al. (2010) EMBO J. 29:1792.
  5. Weidberg, H. et al. (2011) Ann. Rev. Biochem. 80:125.

Long Name

gamma-Aminobutyric Acid Type A Receptor Associated Protein

Alternate Names

Apg8p1, ATG8A, FLC3B, MM46

Entrez Gene IDs

11337 (Human); 56486 (Mouse); 58974 (Rat)

Gene Symbol

GABARAP

UniProt

NP_009209 (Human)

Additional GABARAP Products

Product Documents for Recombinant Human His6-GABARAP Protein, CF

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Product Specific Notices for Recombinant Human His6-GABARAP Protein, CF

For research use only

Citations for Recombinant Human His6-GABARAP Protein, CF

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