Recombinant Human His6-HR6A/UBE2A Protein, CF

Name: Recombinant Human His6-HR6A/UBE2A Protein, CF
Brand: R&D Systems
SKU: E2-612
Price: 272 USD

Catalog #: E2-612
1 Citation Datasheet / COA / SDS

Discontinued Product

E2-612 has been discontinued.

View all HR6A/UBE2A products.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human His6-HR6A/UBE2A Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Activity

Recombinant Human His6-HR6A/UBE2A is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-HR6A/UBE2A concentration of 0.1-1 μM.

Source

E. coli-derived human HR6A/UBE2A protein
Contains an N-terminal 6-His tag

Accession #

NP_003327

Predicted Molecular Mass

19 kDa

Product Datasheets

E2-612

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E2-612

Formulation	X mg/ml (XμM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Reconstitution Calculator

Background: HR6A/UBE2A

Homolog of Rad6 A (HR6A), also known as Ubiquitin-conjugating Enzyme E2A (UBE2A), is a ubiquitously expressed member of the Ubiquitin-conjugating (E2) enzyme family that is highly expressed in the brain and heart (1). HR6A/UBE2A has a predicted molecular weight of 17 kDa. The human protein shares 100% amino acid sequence identity with the mouse and rat orthologs. Like other E2 family members, HR6A/UBE2A has an E2 catalytic core domain that contains an active site cysteine residue required for the formation of a thioester bond with Ubiquitin (2). HR6A/UBE2A is involved in many cellular processes including protein degradation, transcriptional regulation, and the DNA damage response. HR6A/UBE2A functions with the UBR2 Ubiquitin ligase (E3) to mediate ubiquitination of target substrates degraded through the N-end rule proteolytic pathway (3). HR6A/UBE2A also interacts with the human BRE1 E3 to ubiquitinate Histone H2B on Lys120, which promotes transcription via methylation of Histone H3 on Lys4 (4). HR6A/UBE2A is activated via phosphorylation on Ser120 by CDK9 following exposure to UVC irradiation and HR6A/UBE2A-RAD18-dependent ubiquitination of PCNA is required for DNA damage tolerance (5-7). HR6A/UBE2A also regulates the mRNA and protein levels of p53 (8). Physiologically, this enzyme is required in female mice for the production of viable offspring (9). Mutations in human HR6A/UBE2A cause UBE2A deficiency syndrome in males, which is characterized by intellectual disability, seizures, absent speech, and urogenital and skin anomalies (10-12). This protein has an N-terminal His₆-tag. (Accession NP_003327)

References

Koken, M.H. et al. (1996) Dev. Biol. 173:119.
Hershko, A. et al. (1983) J. Biol. Chem. 258:8206.
Lee, P.C. et al. (2011) Mol. Cell 43:392.
Kim, J. et al. (2009) Cell 137:459.
Unk, I. et al. (2006) Proc. Natl. Acad. Sci. USA 103:18107.
Yoon, J.H. et al. (2012) Proc. Natl. Acad. Sci. USA 109:7799.
Shchebet, A. et al. (2012) Cell Cycle 11:2122.
Chen, S. et al. (2012) Mol. Cell. Biol. 32:576.
Roest, H.P. et al. (2004) Mol. Cell. Biol. 24:5485.
Nascimento, R.M. et al. (2006) Am. J. Hum. Genet. 79:549.
Honda, S. et al. (2010) J. Hum. Genet. 55:244.
de Leeuw, N. et al. (2010) Am. J. Med. Genet. 152A:3084.

Long Name

Ubiquitin-conjugating Enzyme E2A

Entrez Gene IDs

7319 (Human); 22209 (Mouse); 298317 (Rat)

Alternate Names

EC 6.3.2.19; HHR6A; HR6A; RAD6A; RAD6ARAD6 homolog A; UBC2; UBE2A; Ubiquitin carrier protein A; ubiquitin-conjugating enzyme E2 A; ubiquitin-conjugating enzyme E2A (RAD6 homolog); Ubiquitin-protein ligase A

Related Research Areas

Citation for Recombinant Human His6-HR6A/UBE2A Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1