Recombinant Human His6-HR6B/UBE2B Protein, CF

R&D Systems | Catalog # E2-613

R&D Systems
Discontinued Product
E2-613 has been discontinued. View all HR6B/UBE2B products.

Key Product Details

Source

E. coli

Accession Number

NP_003328

Applications

Enzyme Activity
View all HR6B/UBE2B Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human HR6B/UBE2B protein
Contains an N-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

19 kDa

Activity

Recombinant Human His6-HR6B/UBE2B is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-HR6B/UBE2B concentration of 0.1-1 μM.

Formulation, Preparation, and Storage

E2-613
Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: HR6B/UBE2B

Homolog of Rad6 B (HR6B), also known as Ubiquitin-conjugating Enzyme E2B (UBE2B), is a ubiquitously expressed member of the Ubiquitin-conjugating (E2) enzyme family. It has a predicted molecular weight of 17 kDa. The human enzyme shares 100% amino acid sequence identity with the mouse and rat orthologs. HR6B/UBE2B localizes to the nucleus and cytoplasm and is highly expressed in the brain, heart, and testis (1,2). HR6B/UBE2B has an E2 catalytic core domain with an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin (3). PCNA is mono-ubiquitinated by HR6B/UBE2B via the RAD18 Ubiquitin ligase (E3) (4). HR6B/UBE2B also functions with the UBR2 E3 to ubiquitinate Histone H2A (5). HR6B/UBE2B plays important roles during spermatogenesis and is required for fertility in male mice (6-10). Pathologically, HR6B/UBE2B is overexpressed in mouse and human breast cancer cell lines and tumors and has been reported to provide resistance to chemotherapy drugs (2,11). This protein has an N-terminal His6-tag. (Accession NP_003328)

References

  1. Koken, M.H. et al. (1996) Dev. Biol. 173:119.
  2. Shekhar, M.P. et al. (2002) Cancer Res. 62:2115.
  3. Hershko, A. et al. (1983) J. Biol. Chem. 258:8206.
  4. Hibbert, R.G. et al. (2011) Proc. Natl. Acad. Sci. USA 108:5590.
  5. An, J.Y. et al. (2010) Proc. Natl. Acad. Sci. USA 107:1912.
  6. Roest, H.P. et al. (1996) Cell 86:799.
  7. Baarends, W.M. et al. (2003) Mol. Cell. Biol. 23:1151.
  8. Mulugeta Achame, E.M. et al. (2010) BMC Genomics 10:367.
  9. Inagaki, A. et al. (2011) J. Cell Sci. 124:2837.
  10. An, J.Y. et al. (2012) PLoS One 7:e37414.
  11. Lyakhovich, A. et al. (2004) Oncogene 23:3097.

Long Name

Ubiquitin-conjugating Enzyme E2B

Alternate Names

E2-17k, UBE2B

Entrez Gene IDs

7320 (Human)

Gene Symbol

UBE2B

UniProt

NP_003328

Additional HR6B/UBE2B Products

Product Documents for Recombinant Human His6-HR6B/UBE2B Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human His6-HR6B/UBE2B Protein, CF

For research use only

Related Research Areas

Epigenetics
p53 Pathway

Citations for Recombinant Human His6-HR6B/UBE2B Protein, CF

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Associated Pathways

Ubiquitination Cascade Pathway Ubiquitination Cascade Pathway Thumbnail