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Recombinant Human His6-HR6B/UBE2B Protein, CF

Catalog #: E2-613
1 Citation Datasheet / COA / SDS

Discontinued Product

E2-613 has been discontinued.
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Recombinant Human His6-HR6B/UBE2B Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
Recombinant Human His6-HR6B/UBE2B is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-HR6B/UBE2B concentration of 0.1-1 μM.
Source
E. coli-derived human HR6B/UBE2B protein
Contains an N-terminal 6-His tag
Accession #
NP_003328
Predicted Molecular Mass
19 kDa

Product Datasheets

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E2-613

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E2-613

Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HR6B/UBE2B

Homolog of Rad6 B (HR6B), also known as Ubiquitin-conjugating Enzyme E2B (UBE2B), is a ubiquitously expressed member of the Ubiquitin-conjugating (E2) enzyme family. It has a predicted molecular weight of 17 kDa. The human enzyme shares 100% amino acid sequence identity with the mouse and rat orthologs. HR6B/UBE2B localizes to the nucleus and cytoplasm and is highly expressed in the brain, heart, and testis (1,2). HR6B/UBE2B has an E2 catalytic core domain with an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin (3). PCNA is mono-ubiquitinated by HR6B/UBE2B via the RAD18 Ubiquitin ligase (E3) (4). HR6B/UBE2B also functions with the UBR2 E3 to ubiquitinate Histone H2A (5). HR6B/UBE2B plays important roles during spermatogenesis and is required for fertility in male mice (6-10). Pathologically, HR6B/UBE2B is overexpressed in mouse and human breast cancer cell lines and tumors and has been reported to provide resistance to chemotherapy drugs (2,11). This protein has an N-terminal His6-tag. (Accession NP_003328)

References
  1. Koken, M.H. et al. (1996) Dev. Biol. 173:119.
  2. Shekhar, M.P. et al. (2002) Cancer Res. 62:2115.
  3. Hershko, A. et al. (1983) J. Biol. Chem. 258:8206.
  4. Hibbert, R.G. et al. (2011) Proc. Natl. Acad. Sci. USA 108:5590.
  5. An, J.Y. et al. (2010) Proc. Natl. Acad. Sci. USA 107:1912.
  6. Roest, H.P. et al. (1996) Cell 86:799.
  7. Baarends, W.M. et al. (2003) Mol. Cell. Biol. 23:1151.
  8. Mulugeta Achame, E.M. et al. (2010) BMC Genomics 10:367.
  9. Inagaki, A. et al. (2011) J. Cell Sci. 124:2837.
  10. An, J.Y. et al. (2012) PLoS One 7:e37414.
  11. Lyakhovich, A. et al. (2004) Oncogene 23:3097.
Long Name
Ubiquitin-conjugating Enzyme E2B
Entrez Gene IDs
7320 (Human)
Alternate Names
E2 protein; E2-17k; E2-17kDa; HHR6B; HR6B; RAD6 homolog B; RAD6BEC 6.3.2.19; UBC2; UBE2B; Ubiquitin carrier protein B; ubiquitin-conjugating enzyme E2 B; Ubiquitin-conjugating enzyme E2-17 kDa; ubiquitin-conjugating enzyme E2B (RAD6 homolog); Ubiquitin-protein ligase B

Citation for Recombinant Human His6-HR6B/UBE2B Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. ASH2L regulates ubiquitylation signaling to MLL: trans-regulation of H3 K4 methylation in higher eukaryotes.
    Authors: Wu L, Lee S, Zhou B, Nguyen U, Muir T, Tan S, Dou Y
    Mol Cell, 2013-02-28;49(6):1108-20.
    Applications: Bioassay

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