Otubain-1, also known as OTUB1 or Ubiquitin Thioesterase, is a member of the conserved ovarian tumor (OTU) protein family. It is a 271 amino acid (aa) polypeptide with a predicted molecular weight of 32 kDa. Otubain-1 is 99% identical at the aa level to its mouse and rat orthologs. It is a deubiquitinating enzyme (DUB) that appears to exhibit specificity for K48 Ubiquitin linkages (1). However, Otubain-1 may also affect K63 linkages independent of DUB activity. For instance, Otubain-1 can directly bind to and suppress UBC13 (UBE2N), a Ubiquitin Ligase (E2) that catalyzes the formation K63-linked Ubiquitin chains in response to DNA damage (2-4).
Recombinant Human His6-Otubain-1 Protein, CF
R&D Systems | Catalog # E-522B
Discontinued Product
E-522B has been discontinued.
View all Otubain-1 products.
Key Product Details
Source
E. coli
Accession Number
Applications
Bioactivity
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Product Specifications
Source
E. coli-derived human Otubain-1 protein
Contains an N-terminal 6-His tag
Contains an N-terminal 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Predicted Molecular Mass
32 kDa
Activity
Recombinant Human His6-Otubain-1 is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial His6-Otubain-1 concentration of 1-5 μM.
Reviewed Applications
Read 2 reviews rated 5 using E-522B in the following applications:
Formulation, Preparation, and Storage
E-522B
| Formulation | X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 1 mM TCEP |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Otubain-1
References
- Wang, T. et al. (2009) J. Mol. Biol. 386:1011.
- Nakada, S. et al. (2010) Nature 466:941
- Wiener, R. et al. (2012) Nature 483:618.
- Sato, Y. et al. (2012) J. Biol. Chem. 287:25860.
Long Name
OTU Domain, Ubiquitin Aldehyde Binding 1
Alternate Names
OTB1, OTU1, OTUB1, Otubain1
Gene Symbol
OTUB1
UniProt
Additional Otubain-1 Products
Product Documents for Recombinant Human His6-Otubain-1 Protein, CF
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human His6-Otubain-1 Protein, CF
For research use only
Related Research Areas
Citations for Recombinant Human His6-Otubain-1 Protein, CF
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Customer Reviews for Recombinant Human His6-Otubain-1 Protein, CF (2)
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2 Customer Ratings
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Associated Pathways
