Recombinant Human His6-Otubain-1 Protein, CF

Catalog # Availability Size / Price Qty
E-522B-050
R&D Systems Recombinant Proteins and Enzymes
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Product Details
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Recombinant Human His6-Otubain-1 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity

Recombinant Human His6-Otubain-1 is a Ubiquitin-specific deconjugating enzyme.  Reaction conditions will need to be optimized for each specific application. We recommend an initial His6-Otubain-1 concentration of 1-5 μM.

Source
E. coli-derived human Otubain-1 protein
Contains an N-terminal 6-His tag
Accession #
Predicted Molecular Mass
32 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-522B

Formulation

X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 1 mM TCEP

Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Otubain-1

Otubain-1, also known as OTUB1 or Ubiquitin Thioesterase, is a member of the conserved ovarian tumor (OTU) protein family.  It is a 271 amino acid (aa) polypeptide with a predicted molecular weight of 32 kDa. Otubain-1 is 99% identical at the aa level to its mouse and rat orthologs. It is a deubiquitinating enzyme (DUB) that appears to exhibit specificity for K48 Ubiquitin linkages (1). However, Otubain-1 may also affect K63 linkages independent of DUB activity.  For instance, Otubain-1 can directly bind to and suppress UBC13 (UBE2N), a Ubiquitin Ligase (E2) that catalyzes the formation K63-linked Ubiquitin chains in response to DNA damage (2-4).

References
  1. Wang, T. et al. (2009) J. Mol. Biol. 386:1011.
  2. Nakada, S. et al. (2010) Nature 466:941
  3. Wiener, R. et al. (2012) Nature 483:618.
  4. Sato, Y. et al. (2012) J. Biol. Chem. 287:25860.
Long Name
OTU Domain, Ubiquitin Aldehyde Binding 1
Entrez Gene IDs
55611 (Human); 107260 (Mouse); 293705 (Rat)
Alternate Names
Deubiquitinating enzyme OTUB1; EC 3.1.2; EC 3.4.19.12; FLJ20113; FLJ40710; hOTU1; MGC111158; OTB1; OTB1MGC4584; OTU domain, ubiquitin aldehyde binding 1; OTU domain-containing ubiquitin aldehyde-binding protein 1; OTU1; OTU1OTU-domain Ubal-binding 1; OTUB1; Otubain1; Otubain-1; ubiquitin thioesterase OTUB1; ubiquitin-specific protease otubain 1; Ubiquitin-specific-processing protease OTUB1

FAQs

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Reviews for Recombinant Human His6-Otubain-1 Protein, CF

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Recombinant Human His6-Otubain-1 Protein, CF
By Anonymous on 01/21/2021
Application: Enzymatic activity in vitro

Recombinant Human His6-Otubain-1 Protein, CF
By Anonymous on 03/20/2019
Application: Enzymatic activity in vitro