Recombinant Human His6-Otubain-1 Protein, CF Summary
Recombinant Human His6-Otubain-1 is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial His6-Otubain-1 concentration of 1-5 μM.
Contains an N-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 1 mM TCEP
|Shipping||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Otubain-1, also known as OTUB1 or Ubiquitin Thioesterase, is a member of the conserved ovarian tumor (OTU) protein family. It is a 271 amino acid (aa) polypeptide with a predicted molecular weight of 32 kDa. Otubain-1 is 99% identical at the aa level to its mouse and rat orthologs. It is a deubiquitinating enzyme (DUB) that appears to exhibit specificity for K48 Ubiquitin linkages (1). However, Otubain-1 may also affect K63 linkages independent of DUB activity. For instance, Otubain-1 can directly bind to and suppress UBC13 (UBE2N), a Ubiquitin Ligase (E2) that catalyzes the formation K63-linked Ubiquitin chains in response to DNA damage (2-4).
- Wang, T. et al. (2009) J. Mol. Biol. 386:1011.
- Nakada, S. et al. (2010) Nature 466:941
- Wiener, R. et al. (2012) Nature 483:618.
- Sato, Y. et al. (2012) J. Biol. Chem. 287:25860.
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