Recombinant Human His6-SENP1 Catalytic Domain Protein, CF Summary
Asp415 - Leu644 with a N-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT and Glycerol.|
|Shipping||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
SUMO/Sentrin Specific Peptidase 1 (SENP1) is a member of the SENP family of proteases (1). SENPs are a group of cysteine-type peptidases that catalyze two essential functions in the SUMO pathways: processing of full-length small Ubiquitin-related modifiers (SUMOs) to their mature forms and deconjugation of SUMOs from SUMOylated proteins. SENP1 is 644 amino acids (aa) in length with a predicted molecular weight of 73.5 kDa. Human SENP1 shares 89% aa sequence identity with the mouse and rat orthologs (1). Mammalian SENPs share a conserved C-terminal catalytic domain while the N-terminal domains have no significant similarity (2). SENP1 has broad specificity for the three mammalian SUMOs (3). It is highly expressed in testis, with lower levels being seen in thymus, pancreas, spleen, liver, ovary, and small intestine (3). SENP1 shuttles between the nucleus and cytoplasm, and it is thought that its localization is vital for the regulation for SUMOylation status of target proteins (2,4). SENP1 has been shown to enhance Androgen Receptor (AR)-dependent transcription via deSUMOylation of HDAC1, a negative regulator of AR (5,6). Consequently, SENP1 is thought to play a role in the development, progression, and metastasis of prostate cancer (6-8). SENP1 has also been shown to regulate Erythropoietin production during hypoxia by maintaining the stability of HIF1 alpha (6).
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- Bailey, D. & P. O'Hare (2004) J. Biol. Chem. 279:692.
- Xu, Z. & S.W. Au (2005) Biochem. J. 386:325.
- Kim, Y.H. et al. (2005) FEBS Lett. 579:6272.
- Cheng, J. et al. (2004) Mol. Cell. Biol. 24:6021.
- Zuo, Y. & J.K. Cheng (2009) Asian J. Androl. 11:36.
- Cheng, J. et al. (2006) Neoplasia 8:667.
- Wang, Q. et al. (2013) Oncogene 32:2493.
Citations for Recombinant Human His6-SENP1 Catalytic Domain Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Discovery of a Dual SENP1 and SENP2 Inhibitor
Authors: M Brand, EB Bommeli, M Rütimann, U Lindenmann, R Riedl
International Journal of Molecular Sciences, 2022;23(20):.
A selective USP1-UAF1 inhibitor links deubiquitination to DNA damage responses.
Authors: Liang, Qin, Dexheimer, Thomas S, Zhang, Ping, Rosenthal, Andrew S, Villamil, Mark A, You, Changjun, Zhang, Qiuting, Chen, Junjun, Ott, Christin, Sun, Hongmao, Luci, Diane K, Yuan, Bifeng, Simeonov, Anton, Jadhav, Ajit, Xiao, Hui, Wang, Yinsheng, Maloney, David J, Zhuang, Zhihao
Nat Chem Biol, 2014;10(4):298-304.
Sample Types: Protein
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