Recombinant Human His6-UAF1 Protein, CF Summary
Recombinant Human UAF1 is an activator of Ubiquitin-specific deconjugating enzymes USP1, USP12, and USP46. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human UAF1 concentration equal to the concentration of deconjugating enzyme present (1:1 stoichiometry) in in vitro deconjugation reactions utilizing Ubiquitin-AMC.
Contains an C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
X mg/ml (X μM) in 50 mM HEPES, pH 7.5, 100 mM NaCl, 10% glycerol, 1 mM TCEP
|Shipping||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
UAF1 (USP1-Associated Factor 1, also known as WD Repeat-containing protein 48 or WDR48) is a positive regulator of at least three deubiquitinating enzymes of the C19 peptidase family including USP1, USP12, and USP46. In the absence of UAF1, all three of these deubiquitinases are almost completely inactive, and available biochemical evidence suggests UAF1 activation occurs by increasing catalytic turnover as opposed to altered substrate affinity (at least in the case of UAF1/USP1) of the enzymes. Most notably, UAF1 strongly activates USP1 deubiquitination of monoubiquitinated FANCD2 and PCNA, thereby playing important roles in DNA damage response and translesion synthesis. The UAF1/USP12 complex has recently been described as a negative regulator of Notch signaling. Disruption of UAF1-dependent USP12 activity interrupts Notch trafficking to the lysosomes, leading to an increased amount of receptor at the cell surface and to higher Notch activity. At the biochemical level, UAF1/USP12 deubiquitinates the non-activated form of Notch in cell culture and in vitro. This recombinant protein contains a C-terminal 6-His tag.
- Cohn M.A., et al. (2009) J Biol. Chem. 284: 5343-5351
- Huang T.T., et al. (2006) Nat. Cell Biol. 8: 339-347
- Joo H-Y., et al. (2011) Biol. Chem. 286: 7190-7201
- Moretti J., et al. (2012) J Biol. Chem. 287: 29429-29441
- Williams S.A, et al. (2011) Cell 146: 918–930
Citation for Recombinant Human His6-UAF1 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Site-specific ubiquitylation and SUMOylation using genetic-code expansion and sortase
Authors: M Fottner, AD Brunner, V Bittl, D Horn-Ghetk, A Jussupow, VRI Kaila, A Bremm, K Lang
Nat. Chem. Biol., 2019;15(3):276-284.
Sample Types: Recombinant Protein
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