Recombinant Human His6-UbcH6/UBE2E1 Protein, CF

Catalog # Availability Size / Price Qty
E2-630-100
R&D Systems Recombinant Proteins and Enzymes
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Citations (2)
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Recombinant Human His6-UbcH6/UBE2E1 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
Recombinant Human His6-UbcH6/UBE2E1 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UbcH6/UBE2E1 concentration of 0.1-1 μM.
Source
E. coli-derived human UbcH6/UBE2E1 protein
Contains an N-terminal Gly-Ser-Ser and 6-His tag
Accession #
Predicted Molecular Mass
23 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E2-630

Formulation

X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP.

Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

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Background: UbcH6/UBE2E1

Ubiquitin-conjugating Enzyme H6 (UbcH6), also known as Ubiquitin-conjugating Enzyme E2E 1 (UBE2E1), is a ubiquitously expressed protein with a predicted molecular weight of 21 kDa. Human UbcH6/UBE2E1 shares 99% and 100% amino acid sequence identity with mouse and rat UBE2E1, respectively. UbcH6/UBE2E1 mediates the selective degradation of short-lived and abnormal proteins (1). UbcH6/UBE2E1 catalytic activity is modulated following the addition of the Ubiquitin-like protein ISG15 to Lys136 (2). In the nucleus, the N-terminal fragment of UbcH6/UBE2E1 interacts with the Ubiquitin ligase (E3) TRIM21 to mediate substrate ubiquitination (3,4). UbcH6/UBE2E1 also interacts with the viral E3, ICP0, during HSV-1 infection (5). In addition, UbcH6/UBE2E1-dependent protein ubiquitination is implicated in polyglutamine tract disorders such as ataxia (6,7).

References
  1. Nuber, U. et al. (1996) J. Biol. Chem. 271: 2795.
  2. Takeuchi, T. et al. (2005) J. Biochem. 138:711.
  3. Espinosa, A. et al. (2008) Exp. Cell Res. 314:3605.
  4. Espinosa, A. et al. (2011) J. Biol. Chem. 286:36478.
  5. Vanni, E. et al. (2012) J. Virol. 86:6323.
  6. Hong, S. et al. (2008) Biochem. Biophys. Res. Commun. 372:256.
  7. Lee, S. et al. (2008) Biochem. Biophys. Res. Commun. 372:735.
  Additional Sources
  1. Ito K., et al. (2001) Eur J. Biochem. 268: 2725-2732
  2. Nuber U., et al. (1996) J. Biol. Chem. 271: 2795-800
  3. Plafker S.M., et al. (2004) J. Cell Biol. 167: 649-659
  4. Rajendra R., et al. (2004) J. Biol. Chem. 279: 36440-36444
  5. Vichi A., et al. (2005) Proc. Natl. Acad. Sci. 102: 1945-1950
  6. Wang Z., et al. (2005) J. Virol. 79: 8764-8772
 
Entrez Gene IDs
7324 (Human); 22194 (Mouse)
Alternate Names
EC 6.3.2.19; UbcH6; UBE2E1; Ubiquitin carrier protein E1; ubiquitin-conjugating enzyme E2 E1; ubiquitin-conjugating enzyme E2E 1 (homologous to yeast UBC4/5); ubiquitin-conjugating enzyme E2E 1 (UBC4/5 homolog, yeast); Ubiquitin-protein ligase E1

Citations for Recombinant Human His6-UbcH6/UBE2E1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Extracellular matrix stiffness determines DNA repair efficiency and cellular sensitivity to genotoxic agents
    Authors: M Deng, J Lin, S Nowsheen, T Liu, Y Zhao, PW Villalta, D Sicard, DJ Tschumperl, S Lee, J Kim, Z Lou
    Science Advances, 2020;6(37):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. CHIP ubiquitylates NOXA and induces its lysosomal degradation in response to DNA damage
    Authors: MC Albert, K Brinkmann, W Pokrzywa, SD Günther, M Krönke, T Hoppe, H Kashkar
    Cell Death & Disease, 2020;11(9):740.
    Species: Human
    Sample Types: Whole Cells
    Applications: Ubiquitylation

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