Recombinant Human His6-UbcH6/UBE2E1 Protein, CF Summary
Contains an N-terminal Gly-Ser-Ser and 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP.
|Shipping||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Ubiquitin-conjugating Enzyme H6 (UbcH6), also known as Ubiquitin-conjugating Enzyme E2E 1 (UBE2E1), is a ubiquitously expressed protein with a predicted molecular weight of 21 kDa. Human UbcH6/UBE2E1 shares 99% and 100% amino acid sequence identity with mouse and rat UBE2E1, respectively. UbcH6/UBE2E1 mediates the selective degradation of short-lived and abnormal proteins (1). UbcH6/UBE2E1 catalytic activity is modulated following the addition of the Ubiquitin-like protein ISG15 to Lys136 (2). In the nucleus, the N-terminal fragment of UbcH6/UBE2E1 interacts with the Ubiquitin ligase (E3) TRIM21 to mediate substrate ubiquitination (3,4). UbcH6/UBE2E1 also interacts with the viral E3, ICP0, during HSV-1 infection (5). In addition, UbcH6/UBE2E1-dependent protein ubiquitination is implicated in polyglutamine tract disorders such as ataxia (6,7).
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- Vanni, E. et al. (2012) J. Virol. 86:6323.
- Hong, S. et al. (2008) Biochem. Biophys. Res. Commun. 372:256.
- Lee, S. et al. (2008) Biochem. Biophys. Res. Commun. 372:735.
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- Nuber U., et al. (1996) J. Biol. Chem. 271: 2795-800
- Plafker S.M., et al. (2004) J. Cell Biol. 167: 649-659
- Rajendra R., et al. (2004) J. Biol. Chem. 279: 36440-36444
- Vichi A., et al. (2005) Proc. Natl. Acad. Sci. 102: 1945-1950
- Wang Z., et al. (2005) J. Virol. 79: 8764-8772
Citations for Recombinant Human His6-UbcH6/UBE2E1 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Extracellular matrix stiffness determines DNA repair efficiency and cellular sensitivity to genotoxic agents
Authors: M Deng, J Lin, S Nowsheen, T Liu, Y Zhao, PW Villalta, D Sicard, DJ Tschumperl, S Lee, J Kim, Z Lou
Science Advances, 2020;6(37):.
Sample Types: Whole Cells
CHIP ubiquitylates NOXA and induces its lysosomal degradation in response to DNA damage
Authors: MC Albert, K Brinkmann, W Pokrzywa, SD Günther, M Krönke, T Hoppe, H Kashkar
Cell Death & Disease, 2020;11(9):740.
Sample Types: Whole Cells
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