Home / UBE2G1 / Recombinant Human His6-UBE2G1 Protein, CF

Recombinant Human His6-UBE2G1 Protein, CF

Catalog #: E2-700
5 Citations Datasheet / COA / SDS

Discontinued Product

E2-700 has been discontinued.
View all UBE2G1 products.
R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
Citations (5)
FAQs
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human His6-UBE2G1 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Recombinant Human His6-UBE2G1 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UBE2G1 concentration of 0.1-1 μM.
Source
E. coli-derived human UBE2G1 protein
Contains an N-terminal 6-His tag
Accession #
P62253
Predicted Molecular Mass
20 kDa

Product Datasheets

You must select a language.

x

E2-700

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E2-700

Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: UBE2G1

Ubiquitin-conjugating Enzyme E2G 1 (UBE2G1) is a 170 amino acid (aa) member of the Ubiquitin-conjugating (E2) enzyme family with a predicted molecular weight of 19.5 kDa. The human protein shares 100% aa sequence identity with the mouse and rat orthologs.  UBE2G1 has an E2 catalytic core domain with an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin (1). The expression level of UBE2G1 is downregulated when miR-199a is overexpressed, suggesting that UBE2G1 mRNA is targeted by miR-199a (2). UBE2G1 has been reported to function with the CRL4(Cdt2) Ubiquitin ligase (E3) to promote the poly-ubiquitination and degradation of Cdt1 (3). Reduced expression of UBE2G1 in medulloblastoma tumors has also been reported, suggesting that it may act as a tumor suppressor (4). This protein contains an N-terminal 6-His tag.

References
  1. Hershko, A. et al. (1983) J. Biol. Chem. 258:8206.
  2. Haghikia, A. et al. (2011) Eur. Heart J. 32:1287.
  3. Shibata, E. et al. (2011) Mol. Cell. Biol. 31:3136.
  4. Cvekl, A. Jr. et al. (2004) Eur. J. Cancer 40:2525.
Long Name
Ubiquitin-conjugating Enzyme E2G 1
Entrez Gene IDs
7326 (Human); 67128 (Mouse); 64631 (Rat)
Alternate Names
E217K; EC 6.3.2.19; UBC7; UBE2G1; UBE2GE217K; Ubiquitin carrier protein G1; ubiquitin-conjugating enzyme E2 G1; ubiquitin-conjugating enzyme E2G 1 (homologous to C. elegans UBC7); ubiquitin-conjugating enzyme E2G 1 (UBC7 homolog, C. elegans); ubiquitin-conjugating enzyme E2G 1 (UBC7 homolog, yeast); Ubiquitin-protein ligase G1

Citations for Recombinant Human His6-UBE2G1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
Filter your results:

Filter by:

  1. Targeting DCAF5 suppresses SMARCB1-mutant cancer by stabilizing SWI/SNF
    Authors: Radko-Juettner, S;Yue, H;Myers, JA;Carter, RD;Robertson, AN;Mittal, P;Zhu, Z;Hansen, BS;Donovan, KA;Hunkeler, M;Rosikiewicz, W;Wu, Z;McReynolds, MG;Roy Burman, SS;Schmoker, AM;Mageed, N;Brown, SA;Mobley, RJ;Partridge, JF;Stewart, EA;Pruett-Miller, SM;Nabet, B;Peng, J;Gray, NS;Fischer, ES;Roberts, CWM;
    Nature
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  2. Mono-ubiquitination empowers ubiquitin chain elongation
    Authors: Wu, K;DeVita, RJ;Pan, ZQ;
    The Journal of biological chemistry
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  3. The E3 ubiquitin ligase component, Cereblon, is an evolutionarily conserved regulator of Wnt signaling
    Authors: C Shen, A Nayak, LR Neitzel, AA Adams, M Silver-Ise, LM Sawyer, H Benchabane, H Wang, N Bunnag, B Li, DT Wynn, F Yang, M Garcia-Con, CH Williams, S Dakshanamu, CC Hong, NG Ayad, AJ Capobianco, Y Ahmed, E Lee, DJ Robbins
    Nature Communications, 2021-09-06;12(1):5263.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  4. Parkin is an E3 ligase for the ubiquitin-like modifier FAT10, which inhibits Parkin activation and mitophagy
    Authors: ND Roverato, C Sailer, N Catone, A Aichem, F Stengel, M Groettrup
    Cell Reports, 2021-03-16;34(11):108857.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Thalidomide promotes degradation of SALL4, a transcription factor implicated in Duane Radial Ray syndrome
    Authors: KA Donovan, J An, RP Nowak, JC Yuan, EC Fink, BC Berry, BL Ebert, ES Fischer
    Elife, 2018-08-01;7(0):.
    Species: Human
    Sample Types: Protein
    Applications: Ubiquitination

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human His6-UBE2G1 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human His6-UBE2G1 Protein, CF and earn rewards!

Have you used Recombinant Human His6-UBE2G1 Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review