Recombinant Human His6-UBE2G1 Protein, CF

R&D Systems | Catalog # E2-700

R&D Systems
Discontinued Product
E2-700 has been discontinued. View all UBE2G1 products.

Key Product Details

Source

E. coli

Accession Number

P62253

Applications

Enzyme Activity
View all UBE2G1 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human UBE2G1 protein
Contains an N-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

20 kDa

Activity

Recombinant Human His6-UBE2G1 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UBE2G1 concentration of 0.1-1 μM.

Formulation, Preparation, and Storage

E2-700
Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: UBE2G1

Ubiquitin-conjugating Enzyme E2G 1 (UBE2G1) is a 170 amino acid (aa) member of the Ubiquitin-conjugating (E2) enzyme family with a predicted molecular weight of 19.5 kDa. The human protein shares 100% aa sequence identity with the mouse and rat orthologs.  UBE2G1 has an E2 catalytic core domain with an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin (1). The expression level of UBE2G1 is downregulated when miR-199a is overexpressed, suggesting that UBE2G1 mRNA is targeted by miR-199a (2). UBE2G1 has been reported to function with the CRL4(Cdt2) Ubiquitin ligase (E3) to promote the poly-ubiquitination and degradation of Cdt1 (3). Reduced expression of UBE2G1 in medulloblastoma tumors has also been reported, suggesting that it may act as a tumor suppressor (4). This protein contains an N-terminal 6-His tag.

References

  1. Hershko, A. et al. (1983) J. Biol. Chem. 258:8206.
  2. Haghikia, A. et al. (2011) Eur. Heart J. 32:1287.
  3. Shibata, E. et al. (2011) Mol. Cell. Biol. 31:3136.
  4. Cvekl, A. Jr. et al. (2004) Eur. J. Cancer 40:2525.

Long Name

Ubiquitin-conjugating Enzyme E2G 1

Alternate Names

E217K

Entrez Gene IDs

7326 (Human); 67128 (Mouse); 64631 (Rat)

Gene Symbol

UBE2G1

UniProt

P62253 (Human)

Additional UBE2G1 Products

Product Documents for Recombinant Human His6-UBE2G1 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human His6-UBE2G1 Protein, CF

For research use only

Citations for Recombinant Human His6-UBE2G1 Protein, CF

Customer Reviews for Recombinant Human His6-UBE2G1 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human His6-UBE2G1 Protein, CF and earn rewards!

Have you used Recombinant Human His6-UBE2G1 Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes

Associated Pathways

Ubiquitination Cascade Pathway Ubiquitination Cascade Pathway Thumbnail