Recombinant Human His6-UBE2G1 Protein, CF
Recombinant Human His6-UBE2G1 Protein, CF Summary
Contains an N-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP|
|Shipping||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Ubiquitin-conjugating Enzyme E2G 1 (UBE2G1) is a 170 amino acid (aa) member of the Ubiquitin-conjugating (E2) enzyme family with a predicted molecular weight of 19.5 kDa. The human protein shares 100% aa sequence identity with the mouse and rat orthologs. UBE2G1 has an E2 catalytic core domain with an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin (1). The expression level of UBE2G1 is downregulated when miR-199a is overexpressed, suggesting that UBE2G1 mRNA is targeted by miR-199a (2). UBE2G1 has been reported to function with the CRL4(Cdt2) Ubiquitin ligase (E3) to promote the poly-ubiquitination and degradation of Cdt1 (3). Reduced expression of UBE2G1 in medulloblastoma tumors has also been reported, suggesting that it may act as a tumor suppressor (4). This protein contains an N-terminal 6-His tag.
- Hershko, A. et al. (1983) J. Biol. Chem. 258:8206.
- Haghikia, A. et al. (2011) Eur. Heart J. 32:1287.
- Shibata, E. et al. (2011) Mol. Cell. Biol. 31:3136.
- Cvekl, A. Jr. et al. (2004) Eur. J. Cancer 40:2525.
Citation for Recombinant Human His6-UBE2G1 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Thalidomide promotes degradation of SALL4, a transcription factor implicated in Duane Radial Ray syndrome
Authors: KA Donovan, J An, RP Nowak, JC Yuan, EC Fink, BC Berry, BL Ebert, ES Fischer
Sample Types: Protein
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