Ubiquitin-conjugating Enzyme E2G 2 (UBE2G2), also known as Ubiquitin-conjugating Enzyme 7 (Ubc7), is a ubiquitously expressed member of the Ubiquitin-conjugating (E2) enzyme family that localizes to both the nucleus and cytoplasm (1,2). UBE2G2 has a predicted molecular weight of 18 kDa. The human protein shares 100% amino acid sequence identity with the mouse and rat orthologs. UBE2G2 interacts with the HRD1 and gp78 Ubiquitin ligases (E3s) to mediate efficient endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins in human cells (3,4). Mechanistically, it has been shown that UBE2G2 forms Lys48-linked Ubiquitin chains on itself prior to gp78-dependent transfer of the Ubiquitin chains to target proteins (5,6). UBE2G2 also interacts with the CRL4(Cdt2) E3 complex to promote the ubiquitination and degradation of Cdt1 following UV irradiation, suggesting that UBE2G2 may function in the response to DNA damage (7). This protein has an N-terminal His6-tag. (Accession # NP_003334)
Recombinant Human His6-UBE2G2 Protein, CF
R&D Systems | Catalog # E2-680
Discontinued Product
E2-680 has been discontinued.
View all UBE2G2 products.
Key Product Details
Source
E. coli
Accession Number
Applications
Enzyme Activity
Loading...
Product Specifications
Source
E. coli-derived human UBE2G2 protein
Contains an N-terminal 6-His tag
Contains an N-terminal 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Predicted Molecular Mass
20 kDa
Activity
Recombinant Human His6-UBE2G2 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UBE2G2 concentration of 0.1-1 μM.
Formulation, Preparation, and Storage
E2-680
| Formulation | X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: UBE2G2
References
- Katsanis, N. & E.M. Fisher (1998) Genomics 51:128.
- Napolitano, L.M. et al. (2011) Biochem. J. 434:309.
- Kikkert, M. et al. (2004) J. Biol. Chem. 279:3525.
- Chen, B. et al. (2006) Proc. Natl. Acad. Sci. USA 103:341.
- Li, W. et al. (2007) Nature 446:333.
- Li, W. et al. (2009) Proc. Natl. Acad. Sci. USA 106:3722.
- Shibata, E. et al. (2011) Mol. Cell. Biol. 31:3136.
Long Name
Ubiquitin-conjugating Enzyme E2G 2
Alternate Names
UBC7
Gene Symbol
UBE2G2
UniProt
Additional UBE2G2 Products
Product Documents for Recombinant Human His6-UBE2G2 Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human His6-UBE2G2 Protein, CF
For research use only
Citations for Recombinant Human His6-UBE2G2 Protein, CF
Powered by Bioz
Customer Reviews for Recombinant Human His6-UBE2G2 Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Human His6-UBE2G2 Protein, CF and earn rewards!
Have you used Recombinant Human His6-UBE2G2 Protein, CF?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Loading...
Associated Pathways
