Recombinant Human His6-UBE2G2 Protein, CF Summary
Contains an N-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP|
|Shipping||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Ubiquitin-conjugating Enzyme E2G 2 (UBE2G2), also known as Ubiquitin-conjugating Enzyme 7 (Ubc7), is a ubiquitously expressed member of the Ubiquitin-conjugating (E2) enzyme family that localizes to both the nucleus and cytoplasm (1,2). UBE2G2 has a predicted molecular weight of 18 kDa. The human protein shares 100% amino acid sequence identity with the mouse and rat orthologs. UBE2G2 interacts with the HRD1 and gp78 Ubiquitin ligases (E3s) to mediate efficient endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins in human cells (3,4). Mechanistically, it has been shown that UBE2G2 forms Lys48-linked Ubiquitin chains on itself prior to gp78-dependent transfer of the Ubiquitin chains to target proteins (5,6). UBE2G2 also interacts with the CRL4(Cdt2) E3 complex to promote the ubiquitination and degradation of Cdt1 following UV irradiation, suggesting that UBE2G2 may function in the response to DNA damage (7). This protein has an N-terminal His6-tag. (Accession # NP_003334)
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- Napolitano, L.M. et al. (2011) Biochem. J. 434:309.
- Kikkert, M. et al. (2004) J. Biol. Chem. 279:3525.
- Chen, B. et al. (2006) Proc. Natl. Acad. Sci. USA 103:341.
- Li, W. et al. (2007) Nature 446:333.
- Li, W. et al. (2009) Proc. Natl. Acad. Sci. USA 106:3722.
- Shibata, E. et al. (2011) Mol. Cell. Biol. 31:3136.
Citation for Recombinant Human His6-UBE2G2 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Phosphorylation of Kif26b Promotes Its Polyubiquitination and Subsequent Proteasomal Degradation during Kidney Development.
Authors: Terabayashi T, Sakaguchi M, Shinmyozu K
PLoS ONE, 2012;7(6):e39714.
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